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NDUFV3 (NM_021075) Human Tagged ORF Clone

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SKU
RC202915
NDUFV3 (Myc-DDK-tagged)-Human NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa (NDUFV3), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "NDUFV3 or NM_021075"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NDUFV3
Synonyms CI-9KD; CI-10k
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202915 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCCCGTGTTTGCTGCGGCAAGGACGAGCCGGGGCGCTGAAGACTATGCTCCAGGAAGCCCAGG
TGTTTCGAGGACTTGCTTCTACGGTTTCTTTGTCTGCGGAATCAGGGAAGAGTGAAAAGGGTCAGCCACA
GAATTCCAAGAAGCAAAGTCCACCAAAAAATGTAGTGGAACCAAAGGAGAGGGGCAAGCTCCTAGCCACC
CAGACAGCAGCTGAATTGTCTAAAAACTTATCTTCACCCAGTTCTTACCCGCCAGCTGTGAATAAGGGCA
GGAAGGTAGCTAGTCCCAGTCCCAGTGGCAGCGTGCTATTCACAGATGAAGGGGTTCCGAAATTTTTGTC
AAGAAAGACTTTGGTAGAGTTTCCACAGAAAGTTCTGTCTCCATTCAGAAAACAGGGCTCTGATTCAGAA
GCTCGTCAGGTGGGTCGGAAAGTGACGTCGCCTTCGTCTTCATCCTCGTCCAGCTCCTCTGATTCTGAAT
CTGATGATGAGGCTGACGTTTCAGAGGTCACTCCTCGAGTGGTGAGCAAAGGCAGAGGGGGGCTTCGAAA
ACCAGAGGCCTCTCATTCCTTTGAAAACAGAGCCCCCCGAGTTACAGTATCAGCAAAAGAGAAAACCTTG
CTGCAGAAGCCGCATGTGGACATTACTGATCCAGAGAAGCCCCACCAGCCAAAGAAGAAAGGGTCCCCTG
CTAAGCCATCAGAAGGCAGGGAAAATGCGAGACCAAAAACCACAATGCCCAGATCTCAAGTAGATGAAGA
GTTTTTGAAGCAAAGTTTAAAGGAAAAACAATTGCAGAAAACATTTAGATTAAATGAAATAGATAAAGAA
AGCCAAAAGCCATTTGAAGTTAAAGGACCCTTACCTGTCCACACAAAATCAGGGTTGTCTGCGCCACCGA
AGGGCAGCCCAGCGCCTGCTGTGTTGGCAGAAGAGGCCAGAGCAGAGGGGCAGCTGCAAGCCAGTCCTCC
TGGGGCGGCAGAGGGGCATCTGGAAAAACCCGTGCCAGAGCCCCAGCGCAAGGCGGCCCCTCCCCTGCCC
AGAAAGGAAACCTCAGGGACGCAGGGAATAGAAGGCCACCTGAAGGGTGGACAGGCAATCGTGGAAGATC
AGATACCACCAAGCAATTTGGAGACAGTTCCTGTTGAGAATAACCACGGTTTCCATGAAAAGACAGCAGC
GCTGAAGCTTGAGGCCGAGGGCGAGGCCATGGAAGATGCAGCCGCGCCAGGGAACGACCGAGGCGGCACA
CAGGAGCCAGCCCCAGTGCCTGCTGAGCCGTTTGACAACACTACCTACAAGAACCTGCAGCATCATGACT
ACAGCACGTACACCTTCTTAGACCTCAACCTCGAACTCTCAAAATTCAGGATGCCTCAGCCCTCCTCAGG
CCGGGAGTCACCTCGACAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202915 protein sequence
Red=Cloning site Green=Tags(s)

MAAPCLLRQGRAGALKTMLQEAQVFRGLASTVSLSAESGKSEKGQPQNSKKQSPPKNVVEPKERGKLLAT
QTAAELSKNLSSPSSYPPAVNKGRKVASPSPSGSVLFTDEGVPKFLSRKTLVEFPQKVLSPFRKQGSDSE
ARQVGRKVTSPSSSSSSSSSDSESDDEADVSEVTPRVVSKGRGGLRKPEASHSFENRAPRVTVSAKEKTL
LQKPHVDITDPEKPHQPKKKGSPAKPSEGRENARPKTTMPRSQVDEEFLKQSLKEKQLQKTFRLNEIDKE
SQKPFEVKGPLPVHTKSGLSAPPKGSPAPAVLAEEARAEGQLQASPPGAAEGHLEKPVPEPQRKAAPPLP
RKETSGTQGIEGHLKGGQAIVEDQIPPSNLETVPVENNHGFHEKTAALKLEAEGEAMEDAAAPGNDRGGT
QEPAPVPAEPFDNTTYKNLQHHDYSTYTFLDLNLELSKFRMPQPSSGRESPRH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021075
ORF Size 1419 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021075.3, NP_066553.3
RefSeq Size 2151 bp
RefSeq ORF 1422 bp
Locus ID 4731
UniProt ID P56181
Cytogenetics 21q22.3
Protein Pathways Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
MW 51 kDa
Summary The protein encoded by this gene is one of at least forty-one subunits that make up the NADH-ubiquinone oxidoreductase complex. This complex is part of the mitochondrial respiratory chain and serves to catalyze the rotenone-sensitive oxidation of NADH and the reduction of ubiquinone. The encoded protein is one of three proteins found in the flavoprotein fraction of the complex. The specific function of the encoded protein is unknown. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
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Documents
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"NM_021075" in other vectors (6)
SKU Description Size Price
RC202915L3 Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa (NDUFV3), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC202915L4 Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa (NDUFV3), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$757.00
RG202915 NDUFV3 (tGFP-tagged) - Human NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa (NDUFV3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$489.00 $657.00
SC113024 NDUFV3 (untagged)-Human NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa (NDUFV3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$657.00
SC317780 NDUFV3 (untagged)-Human NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa (NDUFV3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$503.00
SC320325 NDUFV3 (untagged)-Human NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa (NDUFV3), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$457.00

Plasmid Map for RC202915

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202915
Circular map for RC202915

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