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HTR1D (NM_000864) Human Tagged ORF Clone

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SKU
RC202862
HTR1D (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 1D (HTR1D)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HTR1D
Synonyms 5-HT1D; HT1DA; HTR1DA; HTRL; RDC4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202862 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCCACTGAACCAGTCAGCAGAAGGCCTTCCCCAGGAGGCCTCCAACAGATCCCTGAATGCCACAG
AAACCTCAGAGGCTTGGGATCCCAGGACCCTCCAGGCGCTCAAGATCTCCCTTGCCGTGGTCCTTTCCGT
CATCACACTGGCCACAGTCCTCTCCAATGCCTTTGTACTCACCACCATCTTACTCACCAGGAAGCTCCAC
ACCCCTGCCAACTACCTGATTGGCTCCCTGGCCACCACCGACCTCTTGGTTTCCATCTTGGTAATGCCCA
TCAGCATCGCCTATACCATCACCCACACCTGGAACTTTGGCCAAATCTTGTGTGACATCTGGCTGTCCTC
TGACATCACGTGCTGCACAGCCTCCATCCTGCATCTCTGTGTCATTGCTCTGGACAGGTACTGGGCAATC
ACAGATGCCCTGGAATACAGTAAACGCAGGACGGCTGGCCACGCGGCCACCATGATCGCCATTGTCTGGG
CCATCTCCATCTGCATCTCCATCCCCCCGCTCTTCTGGCGGCAGGCCAAGGCCCAGGAGGAGATGTCGGA
CTGTCTGGTGAACACCTCTCAGATTTCCTACACCATCTACTCCACCTGTGGGGCCTTCTACATTCCCTCG
GTGTTGCTCATCATCCTATATGGCCGGATCTACCGGGCTGCCCGGAACCGCATCCTGAATCCACCCTCAC
TCTATGGGAAGCGCTTCACCACGGCCCACCTCATCACAGGCTCTGCCGGGTCCTCGCTCTGCTCGCTCAA
CTCCAGCCTCCATGAGGGGCACTCGCACTCGGCTGGCTCCCCTCTCTTTTTCAACCACGTGAAAATCAAG
CTTGCTGACAGTGCCCTGGAACGCAAGAGGATTTCTGCTGCTCGAGAAAGGAAAGCCACTAAAATCCTGG
GCATCATTCTGGGGGCCTTTATCATCTGCTGGCTGCCCTTCTTCGTGGTGTCTCTGGTCCTCCCCATCTG
CCGGGACTCCTGCTGGATCCACCCGGCGCTCTTTGACTTCTTCACCTGGCTAGGCTATTTAAACTCCCTC
ATCAATCCAATAATCTACACTGTGTTTAATGAAGAGTTTCGGCAAGCTTTTCAGAAAATTGTCCCTTTCC
GGAAGGCCTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202862 protein sequence
Red=Cloning site Green=Tags(s)

MSPLNQSAEGLPQEASNRSLNATETSEAWDPRTLQALKISLAVVLSVITLATVLSNAFVLTTILLTRKLH
TPANYLIGSLATTDLLVSILVMPISIAYTITHTWNFGQILCDIWLSSDITCCTASILHLCVIALDRYWAI
TDALEYSKRRTAGHAATMIAIVWAISICISIPPLFWRQAKAQEEMSDCLVNTSQISYTIYSTCGAFYIPS
VLLIILYGRIYRAARNRILNPPSLYGKRFTTAHLITGSAGSSLCSLNSSLHEGHSHSAGSPLFFNHVKIK
LADSALERKRISAARERKATKILGIILGAFIICWLPFFVVSLVLPICRDSCWIHPALFDFFTWLGYLNSL
INPIIYTVFNEEFRQAFQKIVPFRKAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000864
ORF Size 1131 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000864.3, NP_000855.1
RefSeq Size 2857 bp
RefSeq ORF 1134 bp
Locus ID 3352
UniProt ID P28221
Cytogenetics 1p36.12
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 41.9 kDa
Summary G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Regulates the release of 5-hydroxytryptamine in the brain, and thereby affects neural activity. May also play a role in regulating the release of other neurotransmitters. May play a role in vasoconstriction.UniProtKB/Swiss-Prot Function
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Documents
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"NM_000864" in other vectors (4)
SKU Description Size Price
RC202862L3 Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 1D (HTR1D), Myc-DDK-tagged 10 ug
$757.00
RC202862L4 Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 1D (HTR1D), mGFP tagged 10 ug
$757.00
RG202862 HTR1D (tGFP-tagged) - Human 5-hydroxytryptamine (serotonin) receptor 1D (HTR1D) 10 ug
$489.00 $657.00
SC119613 HTR1D (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 1D (HTR1D) 10 ug
$457.00

Plasmid Map for RC202862

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202862
Circular map for RC202862

file-alt Citations

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