GNG3 (NM_012202) Human Tagged ORF Clone

  • Product Brand Image
SKU
RC202857
GNG3 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), gamma 3 (GNG3)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GNG3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202857 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGGTGAGACCCCGGTGAACAGCACTATGAGTATTGGGCAAGCACGCAAGATGGTGGAACAGCTTA
AGATTGAAGCCAGCTTGTGTCGGATAAAGGTGTCCAAGGCAGCAGCAGACCTGATGACTTACTGTGATGC
CCACGCCTGTGAGGATCCCCTCATCACCCCTGTGCCCACTTCGGAGAACCCCTTCCGGGAGAAGAAGTTC
TTCTGTGCTCTCCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202857 protein sequence
Red=Cloning site Green=Tags(s)

MKGETPVNSTMSIGQARKMVEQLKIEASLCRIKVSKAAADLMTYCDAHACEDPLITPVPTSENPFREKKF
FCALL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012202
ORF Size 225 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012202.5
RefSeq Size 962 bp
RefSeq ORF 228 bp
Locus ID 2785
UniProt ID P63215
Cytogenetics 11q12.3
Domains G-gamma
Protein Families Druggable Genome
Protein Pathways Chemokine signaling pathway, Taste transduction
MW 8.3 kDa
Summary Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits. The gamma subunit determines the specificity of which signaling pathways will be affected by this particular complex. The protein encoded by this gene represents the gamma subunit of both inhibitory and stimulatory complexes. provided by RefSeq, Jan 2012
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_012202" in other vectors (4)
SKU Description Size Price
RC202857L3 Lenti ORF clone of Human guanine nucleotide binding protein (G protein), gamma 3 (GNG3), Myc-DDK-tagged 10 ug
$450.00
RC202857L4 Lenti ORF clone of Human guanine nucleotide binding protein (G protein), gamma 3 (GNG3), mGFP tagged 10 ug
$450.00
RG202857 GNG3 (tGFP-tagged) - Human guanine nucleotide binding protein (G protein), gamma 3 (GNG3) 10 ug
$489.00
SC115485 GNG3 (untagged)-Human guanine nucleotide binding protein (G protein), gamma 3 (GNG3) 10 ug
$150.00

Plasmid Map for RC202857

Download sequence for

Cloning scheme for RC202857

Circular map for RC202857

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products