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NDFIP1 (NM_030571) Human Tagged ORF Clone

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SKU
RC202574
NDFIP1 (Myc-DDK-tagged)-Human Nedd4 family interacting protein 1 (NDFIP1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "NDFIP1 or NM_030571"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NDFIP1
Synonyms N4WBP5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202574 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTTGGCGTTGGCGGCGCTGGCGGCGGTCGAGCCGGCCTGCGGCAGCCGGTACCAGCAGTTGCAGA
ATGAAGAAGAGTCTGGAGAACCTGAACAGGCTGCAGGTGATGCTCCTCCACCTTACAGCAGCATTTCTGC
AGAGAGCGCAGCATATTTTGACTACAAGGATGAGTCTGGGTTTCCAAAGCCCCCATCTTACAATGTAGCT
ACAACACTGCCCAGTTATGATGAAGCGGAGAGGACCAAGGCTGAAGCTACTATCCCTTTGGTTCCTGGGA
GAGATGAGGATTTTGTGGGTCGGGATGATTTTGATGATGCTGACCAGCTGAGGATAGGAAATGATGGGAT
TTTCATGTTAACTTTTTTCATGGCATTCCTCTTTAACTGGATTGGGTTTTTCCTGTCTTTTTGCCTGACC
ACTTCAGCTGCAGGAAGGTATGGGGCCATTTCAGGATTTGGTCTCTCTCTAATTAAATGGATCCTGATTG
TCAGGTTTTCCACCTATTTCCCTGGATATTTTGATGGTCAGTACTGGCTCTGGTGGGTGTTCCTTGTTTT
AGGCTTTCTCCTGTTTCTCAGAGGATTTATCAATTATGCAAAAGTTCGGAAGATGCCAGAAACTTTCTCA
AATCTCCCCAGGACCAGAGTTCTCTTTATTTAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202574 protein sequence
Red=Cloning site Green=Tags(s)

MALALAALAAVEPACGSRYQQLQNEEESGEPEQAAGDAPPPYSSISAESAAYFDYKDESGFPKPPSYNVA
TTLPSYDEAERTKAEATIPLVPGRDEDFVGRDDFDDADQLRIGNDGIFMLTFFMAFLFNWIGFFLSFCLT
TSAAGRYGAISGFGLSLIKWILIVRFSTYFPGYFDGQYWLWWVFLVLGFLLFLRGFINYAKVRKMPETFS
NLPRTRVLFIY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030571
ORF Size 663 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030571.4
RefSeq Size 3599 bp
RefSeq ORF 666 bp
Locus ID 80762
UniProt ID Q9BT67
Cytogenetics 5q31.3
Protein Families Transmembrane
MW 24.9 kDa
Summary The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins. provided by RefSeq, Jul 2008
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"NM_030571" in other vectors (6)
SKU Description Size Price
RC202574L1 Lenti ORF clone of Human Nedd4 family interacting protein 1 (NDFIP1), Myc-DDK-tagged 10 ug
$600.00
RC202574L2 Lenti ORF clone of Human Nedd4 family interacting protein 1 (NDFIP1), mGFP tagged 10 ug
$600.00
RC202574L3 Lenti ORF clone of Human Nedd4 family interacting protein 1 (NDFIP1), Myc-DDK-tagged 10 ug
$600.00
RC202574L4 Lenti ORF clone of Human Nedd4 family interacting protein 1 (NDFIP1), mGFP tagged 10 ug
$600.00
RG202574 NDFIP1 (tGFP-tagged) - Human Nedd4 family interacting protein 1 (NDFIP1) 10 ug
$489.00 $500.00
SC109879 NDFIP1 (untagged)-Human Nedd4 family interacting protein 1 (NDFIP1) 10 ug
$300.00

Plasmid Map for RC202574

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202574
Circular map for RC202574

file-alt Citations

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