USP30 (NM_032663) Human Tagged ORF Clone
$722.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | USP30 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | |
| ACCN | NM_032663 |
| ORF Size | 1551 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_032663.5 |
| RefSeq Size | 3766 bp |
| RefSeq ORF | 1554 bp |
| Locus ID | 84749 |
| UniProt ID | Q70CQ3, A0A024RBM3 |
| Cytogenetics | 12q24.11 |
| Domains | UCH |
| Protein Families | Protease, Transmembrane |
| MW | 58.3 kDa |
| Summary | USP30, a member of the ubiquitin-specific protease family (see USP1, MIM 603478), is a novel mitochondrial deubiquitinating (DUB) enzyme (Nakamura and Hirose, 2008 PubMed 18287522).supplied by OMIM, Dec 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_032663" in other vectors (9)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202538L1 | Lenti ORF clone of Human ubiquitin specific peptidase 30 (USP30), Myc-DDK-tagged | 10 ug |
$1,022.00
|
|
| RC202538L2 | Lenti ORF clone of Human ubiquitin specific peptidase 30 (USP30), mGFP tagged | 10 ug |
$1,022.00
|
|
| RC202538L3 | Lenti ORF clone of Human ubiquitin specific peptidase 30 (USP30), Myc-DDK-tagged | 10 ug |
$1,022.00
|
|
| RC202538L4 | Lenti ORF clone of Human ubiquitin specific peptidase 30 (USP30), mGFP tagged | 10 ug |
$1,022.00
|
|
| RG202538 | USP30 (tGFP-tagged) - Human ubiquitin specific peptidase 30 (USP30) | 10 ug |
$922.00
|
|
| SC125494 | USP30 (untagged)-Human ubiquitin specific peptidase 30 (USP30) | 10 ug |
$711.00
|
|
| SC317867 | USP30 (untagged)-Human ubiquitin specific peptidase 30 (USP30) | 10 ug |
$771.00
|
|
| SC317868 | USP30 (untagged)-Human ubiquitin specific peptidase 30 (USP30) | 10 ug |
$771.00
|
|
| SC320718 | USP30 (untagged)-Human ubiquitin specific peptidase 30 (USP30) | 10 ug |
$711.00
|
|
Citations
USP30 and parkin homeostatically regulate atypical ubiquitin chains on mitochondria
Cunningham, Baughman, Phu et al
Nat. Cell Biol. (2015) 17 (2), 160-9 DOI: 10.1038/ncb3097
Cunningham, Baughman, Phu et al
Nat. Cell Biol. (2015) 17 (2), 160-9 DOI: 10.1038/ncb3097
Cited by 334
AI Summary: OriGene’s USP30 cDNA was used to clone and express recombinant USP30 protein, enabling the study of its role in regulating mitochondrial ubiquitin chains in this research.
Snippet: Cloning, expression and purification of USP30. Complementary DNA of USP30 was obtained from OriGene. A construct expressing residues 65–517 was cloned into a pET-derived vector with an N-terminal tobacco etch virus (TEV)-cleavable 6× His tag. USP30 was expressed in BL21(DE3) Escherichia coli cells and induced with 1 mM isopropyl-β-D-thiogalactoside overnight at 16 °C. …
The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy
Bingol, Tea, Phu et al
Nature (2014) 509 (7505), 370-5 DOI: 10.1038/nature13418
Bingol, Tea, Phu et al
Nature (2014) 509 (7505), 370-5 DOI: 10.1038/nature13418
Cited by 867
AI Summary: OriGene's human USP30 and parkin cDNA products were used to generate constructs for overexpression studies, enabling the investigation of their roles in regulating mitophagy in this paper.
Showing 1-2 of 2 papers.
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