HSD17B14 (NM_016246) Human Tagged ORF Clone

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SKU
RC202531
HSD17B14 (Myc-DDK-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 14 (HSD17B14)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HSD17B14
Synonyms DHRS10; retSDR3; SDR47C1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202531 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTACGGGAACGCGCTATGCCGGGAAGGTGGTGGTCGTGACCGGGGGCGGGCGCGGCATCGGAGCTG
GGATCGTGCGCGCCTTCGTGAACAGCGGGGCCCGAGTGGTTATCTGCGACAAGGATGAGTCTGGGGGCCG
GGCCCTGGAGCAGGAGCTCCCTGGAGCTGTCTTTATCCTCTGTGATGTGACTCAGGAAGATGATGTGAAG
ACCCTGGTTTCTGAGACCATCCGCCGATTTGGCCGCCTGGATTGTGTTGTCAACAACGCTGGCCACCACC
CACCCCCACAGAGGCCTGAGGAGACCTCTGCCCAGGGATTCCGCCAGCTGCTGGAGCTGAACCTACTGGG
GACGTACACCTTGACCAAGCTCGCCCTCCCCTACCTGCGGAAGAGTCAAGGGAATGTCATCAACATCTCC
AGCCTGGTGGGGGCAATCGGCCAGGCCCAGGCAGTTCCCTATGTGGCCACCAAGGGGGCAGTAACAGCCA
TGACCAAAGCTTTGGCCCTGGATGAAAGTCCATATGGTGTCCGAGTCAACTGTATCTCCCCAGGAAACAT
CTGGACCCCGCTGTGGGAGGAGCTGGCAGCCTTAATGCCAGACCCTAGGGCCACAATCCGAGAGGGCATG
CTGGCCCAGCCACTGGGCCGCATGGGCCAGCCCGCTGAGGTCGGGGCTGCGGCAGTGTTCCTGGCCTCCG
AAGCCAACTTCTGCACGGGCATTGAACTGCTCGTGACGGGGGGTGCAGAGCTGGGGTACGGGTGCAAGGC
CAGTCGGAGCACCCCCGTGGACGCCCCCGATATCCCTTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202531 protein sequence
Red=Cloning site Green=Tags(s)

MATGTRYAGKVVVVTGGGRGIGAGIVRAFVNSGARVVICDKDESGGRALEQELPGAVFILCDVTQEDDVK
TLVSETIRRFGRLDCVVNNAGHHPPPQRPEETSAQGFRQLLELNLLGTYTLTKLALPYLRKSQGNVINIS
SLVGAIGQAQAVPYVATKGAVTAMTKALALDESPYGVRVNCISPGNIWTPLWEELAALMPDPRATIREGM
LAQPLGRMGQPAEVGAAAVFLASEANFCTGIELLVTGGAELGYGCKASRSTPVDAPDIPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016246
ORF Size 810 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016246.3
RefSeq Size 1277 bp
RefSeq ORF 813 bp
Locus ID 51171
UniProt ID Q9BPX1, A0A140VJH8
Cytogenetics 19q13.33
Domains adh_short
Protein Families Druggable Genome
MW 28.3 kDa
Summary 17-beta-hydroxysteroid dehydrogenases, such as HSD17B14, are primarily involved in metabolism of steroids at the C17 position and also of other substrates, such as fatty acids, prostaglandins, and xenobiotics (Lukacik et al., 2007 PubMed 17067289).supplied by OMIM, Jun 2009
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Documents
Resources
"NM_016246" in other vectors (4)
SKU Description Size Price
RC202531L3 Lenti ORF clone of Human hydroxysteroid (17-beta) dehydrogenase 14 (HSD17B14), Myc-DDK-tagged 10 ug
$600.00
RC202531L4 Lenti ORF clone of Human hydroxysteroid (17-beta) dehydrogenase 14 (HSD17B14), mGFP tagged 10 ug
$600.00
RG202531 HSD17B14 (tGFP-tagged) - Human hydroxysteroid (17-beta) dehydrogenase 14 (HSD17B14) 10 ug
$489.00 $500.00
SC319513 HSD17B14 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 14 (HSD17B14) 10 ug
$300.00

Plasmid Map for RC202531

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Cloning scheme for RC202531

Circular map for RC202531

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