GPSN2 (TECR) (NM_138501) Human Tagged ORF Clone

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SKU
RC202503
TECR (Myc-DDK-tagged)-Human trans-2,3-enoyl-CoA reductase (TECR), transcript variant 1
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GPSN2
Synonyms GPSN2; MRT14; SC2; TER
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202503 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCATTACGAGGTGGAGATTCTGGACGCAAAGACAAGGGAGAAGCTGTGTTTCTTGGACAAGGTGG
AGCCCCACGCCACCATTGCGGAGATCAAGAACCTCTTCACTAAGACCCATCCGCAGTGGTACCCCGCCCG
CCAGTCCCTCCGCCTGGACCCCAAGGGCAAGTCCCTGAAGGATGAGGATGTTCTGCAGAAGCTGCCCGTG
GGCACCACGGCCACACTGTACTTCCGGGACCTGGGGGCCCAGATCAGCTGGGTGACGGTCTTCCTAACAG
AGTACGCGGGGCCCCTTTTCATCTACCTGCTCTTCTACTTCCGAGTGCCCTTCATCTATGGCCACAAATA
TGACTTTACGTCCAGTCGGCATACAGTGGTGCACCTCGCCTGCATCTGTCACTCATTCCACTACATCAAG
CGCCTGCTGGAGACGCTCTTCGTGCACCGCTTCTCCCATGGCACTATGCCTTTGCGCAACATCTTCAAGA
ACTGCACCTACTACTGGGGCTTCGCCGCGTGGATGGCCTATTACATCAATCACCCTCTCTACACTCCCCC
TACCTACGGAGCTCAGCAGGTGAAACTGGCGCTCGCCATCTTTGTGATCTGCCAGCTCGGCAACTTCTCC
ATCCACATGGCCCTGCGGGACCTGCGGCCCGCTGGGTCCAAGACGCGGAAGATCCCATACCCCACCAAGA
ACCCCTTCACGTGGCTCTTCCTGCTGGTGTCCTGCCCCAACTACACCTACGAGGTGGGGTCCTGGATCGG
TTTCGCCATCATGACGCAGTGTCTCCCAGTGGCCCTGTTCTCCCTGGTGGGCTTCACCCAGATGACCATC
TGGGCCAAGGGCAAGCACCGCAGCTACCTGAAGGAGTTCCGGGACTACCCGCCCCTGCGCATGCCCATCA
TCCCCTTCCTGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202503 protein sequence
Red=Cloning site Green=Tags(s)

MKHYEVEILDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV
GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGHKYDFTSSRHTVVHLACICHSFHYIK
RLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGAQQVKLALAIFVICQLGNFS
IHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTI
WAKGKHRSYLKEFRDYPPLRMPIIPFLL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138501
ORF Size 924 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138501.6
RefSeq Size 1180 bp
RefSeq ORF 927 bp
Locus ID 9524
UniProt ID Q9NZ01
Cytogenetics 19p13.12
Domains Steroid_dh
Protein Families Transmembrane
Protein Pathways Biosynthesis of unsaturated fatty acids
MW 36 kDa
Summary This gene encodes a multi-pass membrane protein that resides in the endoplasmic reticulum, and belongs to the steroid 5-alpha reductase family. The elongation of microsomal long and very long chain fatty acid consists of 4 sequential reactions. This protein catalyzes the final step, reducing trans-2,3-enoyl-CoA to saturated acyl-CoA. Alternatively spliced transcript variants have been found for this gene.provided by RefSeq, Apr 2011
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Documents
Resources
"NM_138501" in other vectors (4)
SKU Description Size Price
RC202503L3 Lenti ORF clone of Human trans-2,3-enoyl-CoA reductase (TECR), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC202503L4 Lenti ORF clone of Human trans-2,3-enoyl-CoA reductase (TECR), transcript variant 1, mGFP tagged 10 ug
$600.00
RG202503 TECR (tGFP-tagged) - Human trans-2,3-enoyl-CoA reductase (TECR), transcript variant 1 10 ug
$489.00 $500.00
SC319230 TECR (untagged)-Human trans-2,3-enoyl-CoA reductase (TECR), transcript variant 1 10 ug
$300.00

Plasmid Map for RC202503

Download sequence for

Cloning scheme for RC202503

Circular map for RC202503

file-alt Citations

Tegavivint triggers TECR-dependent nonapoptotic cancer cell death
Leak, Wang, Joseph et al
Nat Chem Biol (2025)
Cited by 7
AI Summary: OriGene's Myc-DDK-tagged TECR (RC202503) plasmid was used to express TECR in experimental systems, enabling the study of TECR's role in tegavivint-induced nonapoptotic cancer cell death.
Snippet: Myc-DDK-tagged TECR (transcript 1) plasmid was purchased from OriGene Technologies (RC202503). Plasmid DNA was reconstituted in 100 µl of sterile nuclease-free water for a final concentration of 100 ng µl–1, and 1 µl of this solution was used to transform 50 µl of subcloning efficiency DH5α bacteria (18265017, Thermo Fisher Scientific). …
Showing 1-1 of 1 papers.
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