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ARFGAP3 (NM_014570) Human Tagged ORF Clone

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SKU
RC202392
ARFGAP3 (Myc-DDK-tagged)-Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $480.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ARFGAP3
Synonyms ARFGAP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202392 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGACCCCAGCAAGCAGGACATCTTGACCATCTTCAAGCGCCTCCGCTCGGTGCCCACTAACAAGG
TGTGTTTTGATTGTGGTGCCAAAAATCCCAGCTGGGCAAGCATAACCTATGGAGTGTTCCTTTGCATTGA
TTGCTCAGGGTCCCACCGGTCACTTGGTGTTCACTTGAGTTTTATTCGATCTACAGAGTTGGATTCCAAC
TGGTCATGGTTTCAGTTGCGATGCATGCAAGTCGGAGGAAACGCTAGTGCATCTTCCTTTTTTCATCAAC
ATGGGTGTTCCACCAATGACACCAATGCCAAGTACAACAGTCGTGCTGCTCAGCTCTATAGGGAGAAAAT
CAAATCGCTCGCCTCTCAAGCAACACGGAAGCATGGCACTGATCTGTGGCTTGATAGTTGTGTGGTTCCA
CCTTTGTCCCCTCCACCAAAGGAGGAAGATTTTTTTGCCTCTCACGTTTCTCCTGAGGTGAGTGACACAG
CGTGGGCATCAGCAATAGCAGAACCATCTTCTTTAACATCAAGGCCTGTGGAAACCACTTTGGAAAATAA
TGAAGGTGGACAAGAGCAAGGACCAAGTGTGGAAGGTCTTAATGTACCAACAAAGGCTACTTTAGAGGTA
TCCTCTATCATAAAAAAGAAACCAAATCAAGCTAAAAAAGGCCTTGGGGCCAAAAAAGGAAGTTTGGGAG
CTCAGAAACTGGCAAACACATGCTTTAATGAAATTGAAAAACAAGCTCAAGCTGCGGATAAAATGAAGGA
GCAGGAAGACCTGGCCAAGGTGGTATCTAAAGAAGAATCAATTGTTTCATCATTACGATTAGCCTATAAG
GATCTTGAAATTCAAATGAAGAAAGACGAAAAGATGAACATTAGTGGCAAAAAAAATGTTGACTCAGACA
GACTCGGCATGGGATTTGGAAATTGCAGAAGTGTTATTTCACATTCAGTGACTTCAGATATGCAGACCAT
AGAGCAGGAATCACCCATTATGGCAAAACCAAGAAAAAAGTATAATGATGACAGTGACGATTCATATTTT
ACTTCCAGCTCAAGGTACTTTGACGAGCCAGTGGAGTTAAGGAGCAGTTCTTTCTCTAGCTGGGATGACA
GTTCAGATTCCTATTGGAAAAAAGAGACCAGCAAAGATACTGAAACAGTTCTGAAAACCACAGGCTATTC
AGACAGACCTACTGCTCGCCGCAAGCCAGATTATGAGCCAGTTGAAAATACAGATGAGGCCCAGAAGAAG
TTTGGCAATGTCAAGGCCATTTCATCAGATATGTATTTTGGAAGACAATCCCAGGCTGATTATGAGACCA
GGGCCCGCCTAGAGAGGCTGTCAGCAAGTTCCTCCATAAGCTCGGCTGATCTGTTCGAGGAGCCGAGGAA
GCAGCCAGCAGGGAACTACAGCCTGTCCAGCGTGCTGCCCAACGCCCCCGACATGGCGCAGTTCAAGCAG
GGAGTGAGATCGGTTGCTGGAAAACTCTCCGTCTTTGCTAATGGAGTCGTGACTTCAATTCAGGATCGCT
ACGGTTCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202392 protein sequence
Red=Cloning site Green=Tags(s)

MGDPSKQDILTIFKRLRSVPTNKVCFDCGAKNPSWASITYGVFLCIDCSGSHRSLGVHLSFIRSTELDSN
WSWFQLRCMQVGGNASASSFFHQHGCSTNDTNAKYNSRAAQLYREKIKSLASQATRKHGTDLWLDSCVVP
PLSPPPKEEDFFASHVSPEVSDTAWASAIAEPSSLTSRPVETTLENNEGGQEQGPSVEGLNVPTKATLEV
SSIIKKKPNQAKKGLGAKKGSLGAQKLANTCFNEIEKQAQAADKMKEQEDLAKVVSKEESIVSSLRLAYK
DLEIQMKKDEKMNISGKKNVDSDRLGMGFGNCRSVISHSVTSDMQTIEQESPIMAKPRKKYNDDSDDSYF
TSSSRYFDEPVELRSSSFSSWDDSSDSYWKKETSKDTETVLKTTGYSDRPTARRKPDYEPVENTDEAQKK
FGNVKAISSDMYFGRQSQADYETRARLERLSASSSISSADLFEEPRKQPAGNYSLSSVLPNAPDMAQFKQ
GVRSVAGKLSVFANGVVTSIQDRYGS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014570
ORF Size 1548 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014570.2
RefSeq Size 2851 bp
RefSeq ORF 1551 bp
Locus ID 26286
UniProt ID Q9NP61, A0A024R4U0
Cytogenetics 22q13.2
Domains ArfGap
Protein Pathways Endocytosis
MW 57 kDa
Summary The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. provided by RefSeq, Nov 2008
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Documents
Resources
"NM_014570" in other vectors (5)
SKU Description Size Price
RC202392L3 Lenti ORF clone of Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1, Myc-DDK-tagged 10 ug
$780.00
RC202392L4 Lenti ORF clone of Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1, mGFP tagged 10 ug
$780.00
RG202392 ARFGAP3 (tGFP-tagged) - Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1 10 ug
$489.00 $680.00
SC114939 ARFGAP3 (untagged)-Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1 10 ug
$481.00
SC324381 ARFGAP3 (untagged)-Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1 10 ug
$481.00

Plasmid Map for RC202392

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202392
Circular map for RC202392

file-alt Citations

ArfGAP1 acts as a GTPase-activating protein for human ADP-ribosylation factor-like 1 protein
Feng, Cheng, Hsiao et al
FASEB J (2021) 35 (4), e21337 DOI: 10.1096/fj.202000818RR
AI Summary: OriGene products ArfGAP2 (RC204908) and ArfGAP3 (RC202392) were used as controls to compare the specific GAP activity of ArfGAP1 in regulating Arl1 function in this study.
Snippet: ArfGAP2 and ArfGAP3 were purchased from OriGene Technologies (RC204908 and RC202392, respectively)
Showing 1-1 of 1 papers.
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