UBLCP1 (NM_145049) Human Tagged ORF Clone

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SKU
RC202342
UBLCP1 (Myc-DDK-tagged)-Human ubiquitin-like domain containing CTD phosphatase 1 (UBLCP1)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol UBLCP1
Synonyms CPUB1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202342 representing NM_145049
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTCCCTATCATTGTAAAATGGGGTGGACAGGAGTATTCAGTGACCACACTTTCAGAAGATGATA
CTGTGCTCGATCTCAAACAGTTTCTCAAGACCCTTACAGGAGTTCTTCCAGAACGCCAAAAGTTACTTGG
ACTCAAAGTTAAAGGCAAACCTGCAGAAAATGATGTTAAGCTTGGAGCTCTCAAACTGAAACCAAATACT
AAAATCATGATGATGGGAACTCGTGAGGAGAGCTTGGAAGATGTCTTAGGTCCACCCCCTGACAATGATG
ATGTTGTTAATGACTTTGATATTGAAGATGAAGTAGTTGAAGTAGAAAATAGGGAAGAAAACCTACTGAA
AATTTCTCGCAGAGTGAAAGAGTACAAAGTGGAAATTTTGAATCCTCCCAGGGAAGGGAAAAAGCTTTTG
GTGCTAGATGTTGATTATACATTATTTGACCACAGGTCTTGTGCAGAGACTGGGGTAGAATTAATGCGGC
CATATCTTCATGAATTTCTAACATCTGCCTATGAAGATTATGACATTGTTATTTGGTCTGCAACAAATAT
GAAGTGGATTGAAGCTAAAATGAAAGAGCTGGGAGTGAGCACAAATGCAAATTATAAGATTACTTTCATG
TTGGATAGTGCTGCTATGATAACAGTACATACTCCAAGGAGAGGATTAATAGACGTAAAGCCTCTTGGTG
TTATATGGGGAAAGTTTTCGGAGTTTTACAGCAAGAAAAACACCATTATGTTTGATGACATAGGGAGAAA
TTTTCTAATGAACCCACAGAATGGACTAAAGATAAGGCCTTTTATGAAAGCGCACCTAAATCGTGATAAA
GACAAAGAACTTTTAAAATTAACTCAGTACCTCAAGGAGATAGCAAAATTAGATGACTTTTTGGATCTAA
ATCACAAATATTGGGAAAGATATCTCTCAAAGAAGCAAGGACAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202342 representing NM_145049
Red=Cloning site Green=Tags(s)

MALPIIVKWGGQEYSVTTLSEDDTVLDLKQFLKTLTGVLPERQKLLGLKVKGKPAENDVKLGALKLKPNT
KIMMMGTREESLEDVLGPPPDNDDVVNDFDIEDEVVEVENREENLLKISRRVKEYKVEILNPPREGKKLL
VLDVDYTLFDHRSCAETGVELMRPYLHEFLTSAYEDYDIVIWSATNMKWIEAKMKELGVSTNANYKITFM
LDSAAMITVHTPRRGLIDVKPLGVIWGKFSEFYSKKNTIMFDDIGRNFLMNPQNGLKIRPFMKAHLNRDK
DKELLKLTQYLKEIAKLDDFLDLNHKYWERYLSKKQGQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145049
ORF Size 954 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145049.5
RefSeq Size 2178 bp
RefSeq ORF 957 bp
Locus ID 134510
UniProt ID Q8WVY7
Cytogenetics 5q33.3
Domains CPDc, UBQ
Protein Families Druggable Genome, Protease
MW 36.6 kDa
Summary Dephosphorylates 26S nuclear proteasomes, thereby decreasing their proteolytic activity. The dephosphorylation may prevent assembly of the core and regulatory particles (CP and RP) into mature 26S proteasome.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145049" in other vectors (5)
SKU Description Size Price
RC202342L3 Lenti ORF clone of Human ubiquitin-like domain containing CTD phosphatase 1 (UBLCP1), Myc-DDK-tagged 10 ug
$600.00
RC202342L4 Lenti ORF clone of Human ubiquitin-like domain containing CTD phosphatase 1 (UBLCP1), mGFP tagged 10 ug
$600.00
RG202342 UBLCP1 (tGFP-tagged) - Human ubiquitin-like domain containing CTD phosphatase 1 (UBLCP1) 10 ug
$489.00 $500.00
SC100058 UBLCP1 (untagged)-Human ubiquitin-like domain containing CTD phosphatase 1 (UBLCP1) 10 ug
$300.00
SC322355 UBLCP1 (untagged)-Human ubiquitin-like domain containing CTD phosphatase 1 (UBLCP1) 10 ug
$300.00

Plasmid Map for RC202342

Download sequence for

Cloning scheme for RC202342

Circular map for RC202342

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