SYPL1 (NM_182715) Human Tagged ORF Clone

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SKU
RC202334
SYPL1 (Myc-DDK-tagged)-Human synaptophysin-like 1 (SYPL1), transcript variant 2
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SYPL1
Synonyms H-SP1; SYPL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202334 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGGCTTCCAGATCAACCTCAACCCGCTCAAGGAGCCACTCGGCTTCATCAAGGTCCTCGAGTGGA
TTGCTTCTATCTTTGCTTTTGCCACCTGTGGAGGTTTTAAGGGCCAAACAGAAATTCAAGTGAATTGTCC
TCCTGCAGTTACTGAGAATAAAACTGTTACAGCTACTTTTGGTTATCCATTCAGGTTGAATGAGGCATCA
TTTCAGCCACCTCCAGGTGTAAACATATGTGATGTAAATTGGAAAGATTACGTCCTCATAGGCGATTACT
CTTCTTCTGCACAATTCTATGTTACCTTTGCAGTCTTTGTGTTCCTGTACTGCATTGCTGCCCTTCTGCT
TTATGTTGGCTACACGAGTCTGTATCTGGATAGTCGTAAACTTCCTATGATAGACTTTGTTGTTACACTT
GTTGCCACTTTTTTGTGGTTGGTGAGCACTTCAGCCTGGGCTAAAGCTCTGACAGATATTAAAATAGCTA
CTGGTCACAATATTATTGATGAACTTCCGCCTTGTAAGAAGAAAGCAGTACTGTGTTACTTTGGCTCTGT
GACCAGTATGGGATCCCTAAATGTATCTGTGATATTTGGCTTTCTAAATATGATACTCTGGGGAGGAAAT
GCTTGGTTTGTGTACAAGGAGACCAGCCTACACAGTCCATCAAATACATCTGCCCCTCATAGCCAAGGAG
GTATTCCACCTCCTACCGGAATA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202334 protein sequence
Red=Cloning site Green=Tags(s)

MSGFQINLNPLKEPLGFIKVLEWIASIFAFATCGGFKGQTEIQVNCPPAVTENKTVTATFGYPFRLNEAS
FQPPPGVNICDVNWKDYVLIGDYSSSAQFYVTFAVFVFLYCIAALLLYVGYTSLYLDSRKLPMIDFVVTL
VATFLWLVSTSAWAKALTDIKIATGHNIIDELPPCKKKAVLCYFGSVTSMGSLNVSVIFGFLNMILWGGN
AWFVYKETSLHSPSNTSAPHSQGGIPPPTGI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182715
ORF Size 723 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182715.4
RefSeq Size 2300 bp
RefSeq ORF 726 bp
Locus ID 6856
UniProt ID Q16563
Cytogenetics 7q22.3
Protein Families Transmembrane
MW 26.4 kDa
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Documents
Resources
"NM_182715" in other vectors (5)
SKU Description Size Price
RC202334L3 Lenti ORF clone of Human synaptophysin-like 1 (SYPL1), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC202334L4 Lenti ORF clone of Human synaptophysin-like 1 (SYPL1), transcript variant 2, mGFP tagged 10 ug
$600.00
RG202334 SYPL1 (tGFP-tagged) - Human synaptophysin-like 1 (SYPL1), transcript variant 2 10 ug
$489.00 $500.00
SC107500 SYPL1 (untagged)-Human synaptophysin-like 1 (SYPL1), transcript variant 2 10 ug
$300.00
SC323768 SYPL1 (untagged)-Human synaptophysin-like 1 (SYPL1), transcript variant 2 10 ug
$300.00

Plasmid Map for RC202334

Download sequence for

Cloning scheme for RC202334

Circular map for RC202334

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