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Gemin 6 (GEMIN6) (NM_024775) Human Tagged ORF Clone

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SKU
RC202296
GEMIN6 (Myc-DDK-tagged)-Human gem (nuclear organelle) associated protein 6 (GEMIN6)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Gemin 6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202296 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGAATGGATGAAGAAAGGCCCCTTAGAATGGCAAGATTACATTTACAAAGAGGTCCGAGTGACAG
CCAGTGAGAAGAATGAGTATAAAGGATGGGTTTTAACTACAGACCCAGTCTCTGCCAATATTGTCCTTGT
GAACTTCCTTGAAGATGGCAGCATGTCTGTGACCGGAATTATGGGACATGCTGTGCAGACTGTTGAAACT
ATGAATGAAGGGGACCATAGAGTGAGGGAGAAGCTGATGCATTTGTTCACGTCTGGAGACTGCAAAGCAT
ACAGCCCAGAGGATCTGGAAGAGAGAAAGAACAGCCTAAAGAAATGGCTTGAGAAGAACCACATCCCCAT
CACTGAACAGGGAGACGCTCCAAGGACTCTCTGTGTGGCTGGGGTCCTGACTATAGACCCACCATATGAT
CCAGAAAATTGCAGCAGCTCTAATGAGATTATTCTGTCGCGTGTTCAGGATCTTATTGAAGGACATCTTA
CAGCTTCCCAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202296 protein sequence
Red=Cloning site Green=Tags(s)

MSEWMKKGPLEWQDYIYKEVRVTASEKNEYKGWVLTTDPVSANIVLVNFLEDGSMSVTGIMGHAVQTVET
MNEGDHRVREKLMHLFTSGDCKAYSPEDLEERKNSLKKWLEKNHIPITEQGDAPRTLCVAGVLTIDPPYD
PENCSSSNEIILSRVQDLIEGHLTASQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024775
ORF Size 501 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024775.8
RefSeq Size 705 bp
RefSeq ORF 504 bp
Locus ID 79833
UniProt ID Q8WXD5
Cytogenetics 2p22.1
Protein Families Druggable Genome, Stem cell - Pluripotency
MW 18.9 kDa
Summary GEMIN6 is part of a large macromolecular complex, localized to both the cytoplasm and the nucleus, that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN (MIM 600354), GEMIN2 (SIP1; MIM 602595), GEMIN3 (DDX20; MIM 606168), GEMIN4 (MIM 606969), and GEMIN5 (MIM 607005).supplied by OMIM, Jul 2002
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"NM_024775" in other vectors (6)
SKU Description Size Price
RC202296L1 Lenti ORF clone of Human gem (nuclear organelle) associated protein 6 (GEMIN6), Myc-DDK-tagged 10 ug
$600.00
RC202296L2 Lenti ORF clone of Human gem (nuclear organelle) associated protein 6 (GEMIN6), mGFP tagged 10 ug
$600.00
RC202296L3 Lenti ORF clone of Human gem (nuclear organelle) associated protein 6 (GEMIN6), Myc-DDK-tagged 10 ug
$600.00
RC202296L4 Lenti ORF clone of Human gem (nuclear organelle) associated protein 6 (GEMIN6), mGFP tagged 10 ug
$600.00
RG202296 GEMIN6 (tGFP-tagged) - Human gem (nuclear organelle) associated protein 6 (GEMIN6) 10 ug
$500.00
SC122968 GEMIN6 (untagged)-Human gem (nuclear organelle) associated protein 6 (GEMIN6) 10 ug
$300.00

Plasmid Map for RC202296

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202296
Circular map for RC202296

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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