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LEO1 (NM_138792) Human Tagged ORF Clone

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SKU
RC202274
LEO1 (Myc-DDK-tagged)-Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $620.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "LEO1 or NM_138792"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LEO1
Synonyms RDL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202274 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGATATGGAGGATCTCTTCGGGAGCGACGCCGACAGCGAAGCTGAGCGTAAAGATTCTGATTCTG
GATCTGACTCAGATTCTGATCAAGAGAATGCTGCCTCTGGCAGTAATGCCTCTGGAAGTGAAAGTGATCA
GGATGAAAGAGGTGATTCAGGACAACCAAGTAATAAGGAACTGTTTGGAGATGACAGTGAGGACGAGGGA
GCTTCACATCATAGTGGTAGTGATAATCACTCTGAAAGATCAGACAATAGATCAGAAGCTTCTGAGCGTT
CTGACCATGAGGACAATGACCCCTCAGATGTAGATCAGCACAGTGGATCAGAAGCCCCTAATGATGATGA
AGACGAAGGTCATAGATCGGATGGAGGGAGCCATCATTCAGAAGCAGAAGGTTCTGAAAAAGCACATTCA
GATGATGAAAAATGGGGCAGAGAAGATAAAAGTGACCAGTCAGATGATGAAAAGATACAAAATTCTGATG
ATGAGGAGAGGGCACAAGGATCTGATGAAGATAAGCTGCAGAATTCTGACGATGATGAGAAAATGCAGAA
CACAGATGATGAGGAGAGGCCTCAGCTTTCCGATGATGAGAGACAACAGCTATCTGAGGAGGAAAAGGCT
AATTCTGATGATGAACGGCCGGTAGCTTCTGATAATGATGATGAGAAACAGAATTCTGATGATGAAGAAC
AACCACAGCTGTCTGATGAAGAGAAAATGCAAAATTCTGATGATGAAAGGCCACAGGCCTCAGATGAAGA
ACACAGGCATTCAGATGATGAAGAGGAACAGGATCATAAATCAGAATCTGCAAGAGGCAGTGATAGTGAA
GATGAAGTTTTACGAATGAAACGCAAGAATGCGATTGCATCTGATTCAGAAGCGGATAGTGACACTGAGG
TGCCAAAAGATAATAGTGGAACCATGGATTTATTTGGAGGTGCAGATGATATCTCTTCAGGGAGTGATGG
AGAAGACAAACCACCTACTCCAGGACAGCCTGTTGATGAAAATGGATTGCCTCAGGATCAACAGGAAGAG
GAGCCAATTCCTGAGACCAGAATAGAAGTAGAAATACCCAAAGTAAACACTGATTTAGGAAACGACTTAT
ATTTTGTTAAACTGCCCAACTTTCTCAGTGTAGAGCCCAGACCTTTTGATCCTCAGTATTATGAAGATGA
ATTTGAAGATGAAGAAATGCTGGATGAAGAAGGTAGAACCAGGTTAAAATTAAAGGTAGAAAATACTATA
AGATGGAGGATACGCCGAGATGAAGAAGGAAATGAAATTAAAGAAAGCAATGCTCGGATAGTCAAGTGGT
CAGATGGAAGCATGTCCCTGCATTTAGGCAATGAAGTGTTTGATGTGTACAAAGCCCCACTGCAGGGCGA
CCACAATCATCTTTTTATAAGACAAGGTACTGGTCTACAGGGACAAGCAGTCTTTAAAACGAAACTCACC
TTCAGACCTCACTCTACGGACAGTGCCACACATAGAAAGATGACTCTGTCACTTGCAGATAGGTGTTCAA
AGACACAGAAGATTAGAATCTTGCCAATGGCTGGTCGTGATCCTGAATGCCAACGCACAGAAATGATTAA
GAAAGAAGAAGAACGTTTGAGGGCTTCCATACGTAGGGAATCTCAGCAGCGCCGAATGAGAGAGAAACAG
CACCAGCGGGGGCTGAGCGCCAGTTACCTGGAACCTGATCGATACGATGAGGAGGAGGAAGGCGAGGAGT
CCATCAGCTTGGCTGCCATTAAAAACCGATATAAAGGGGGCATTCGAGAGGAACGAGCCAGAATCTATTC
ATCAGACAGTGATGAGGGATCAGAAGAAGATAAAGCTCAAAGATTACTCAAAGCAAAGAAACTTACCAGT
GATGAGGAAGGTGAACCTTCCGGAAAGAGAAAAGCAGAAGATGATGATAAAGCAAATAAAAAGCATAAGA
AGTATGTGATCAGCGATGAAGAGGAAGAAGATGATGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202274 protein sequence
Red=Cloning site Green=Tags(s)

MADMEDLFGSDADSEAERKDSDSGSDSDSDQENAASGSNASGSESDQDERGDSGQPSNKELFGDDSEDEG
ASHHSGSDNHSERSDNRSEASERSDHEDNDPSDVDQHSGSEAPNDDEDEGHRSDGGSHHSEAEGSEKAHS
DDEKWGREDKSDQSDDEKIQNSDDEERAQGSDEDKLQNSDDDEKMQNTDDEERPQLSDDERQQLSEEEKA
NSDDERPVASDNDDEKQNSDDEEQPQLSDEEKMQNSDDERPQASDEEHRHSDDEEEQDHKSESARGSDSE
DEVLRMKRKNAIASDSEADSDTEVPKDNSGTMDLFGGADDISSGSDGEDKPPTPGQPVDENGLPQDQQEE
EPIPETRIEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTI
RWRIRRDEEGNEIKESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLT
FRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQ
HQRGLSASYLEPDRYDEEEEGEESISLAAIKNRYKGGIREERARIYSSDSDEGSEEDKAQRLLKAKKLTS
DEEGEPSGKRKAEDDDKANKKHKKYVISDEEEEDDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138792
ORF Size 1998 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138792.4
RefSeq Size 2202 bp
RefSeq ORF 2001 bp
Locus ID 123169
UniProt ID Q8WVC0
Cytogenetics 15q21.2
Domains Leo1
MW 75.4 kDa
Summary LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 PubMed 15632063).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_138792" in other vectors (6)
SKU Description Size Price
RC202274L1 Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), Myc-DDK-tagged 10 ug
$920.00
RC202274L2 Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), mGFP tagged 10 ug
$920.00
RC202274L3 Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), Myc-DDK-tagged 10 ug
$920.00
RC202274L4 Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), mGFP tagged 10 ug
$920.00
RG202274 LEO1 (tGFP-tagged) - Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1) 10 ug
$489.00 $820.00
SC120657 LEO1 (untagged)-Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1) 10 ug
$611.00

Plasmid Map for RC202274

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202274
Circular map for RC202274

file-alt Citations

PAF1 complex interactions with SETDB1 mediate promoter H3K9 methylation and transcriptional repression of Hoxa9 and Meis1 in acute myeloid leukemia
Ropa, Saha, Chen et al
Oncotarget (2018) 9 (31), 22123-22136 DOI: 10.18632/oncotarget.25204
Snippet: erated using site-directed mutagenesis using the QuikChangeXL kit according to the manufacturer’s protocol (Agilent). CMV-MYC-FLAG-PAF1, CMV-MYC-FLAG-CTR9, and CMV-MYC-FLAG-LEO1 were purchases from Origene. Cell line generation and cell culture conditions Cell lines were generated from either wild type C57Bl/6 (Taconic Farms) mouse bone marrow, Cdc73fl/fl-CreERT2[51] mouse bone marrow, or M1 mu
FigureFigureFigure
Showing 1-1 of 1 papers.
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