ZG16B (NM_145252) Human Tagged ORF Clone

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SKU
RC202244
ZG16B (Myc-DDK-tagged)-Human zymogen granule protein 16 homolog B (rat) (ZG16B)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZG16B
Synonyms EECP; HRPE773; JCLN2; PAUF; PRO1567
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202244 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCCCAGGGGGCCCAGGAGAGTATAAAGGCGATGTGGAGGGTGCCCGGCACAACCAGACGCCCAG
TCACAGGCGAGAGCCCTGGGATGCACCGGCCAGAGGCCATGCTGCTGCTGCTCACGCTTGCCCTCCTGGG
GGGCCCCACCTGGGCAGGGAAGATGTATGGCCCTGGAGGAGGCAAGTATTTCAGCACCACTGAAGACTAC
GACCATGAAATCACAGGGCTGCGGGTGTCTGTAGGTCTTCTCCTGGTGAAAAGTGTCCAGGTGAAACTTG
GAGACTCCTGGGACGTGAAACTGGGAGCCTTAGGTGGGAATACCCAGGAAGTCACCCTGCAGCCAGGCGA
ATACATCACAAAAGTCTTTGTCGCCTTCCAAGCTTTCCTCCGGGGTATGGTCATGTACACCAGCAAGGAC
CGCTATTTCTATTTTGGGAAGCTTGATGGCCAGATCTCCTCTGCCTACCCCAGCCAAGAGGGGCAGGTGC
TGGTGGGCATCTATGGCCAGTATCAACTCCTTGGCATCAAGAGCATTGGCTTTGAATGGAATTATCCACT
AGAGGAGCCGACCACTGAGCCACCAGTTAATCTCACATACTCAGCAAACTCACCCGTGGGTCGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202244 protein sequence
Red=Cloning site Green=Tags(s)

MGAQGAQESIKAMWRVPGTTRRPVTGESPGMHRPEAMLLLLTLALLGGPTWAGKMYGPGGGKYFSTTEDY
DHEITGLRVSVGLLLVKSVQVKLGDSWDVKLGALGGNTQEVTLQPGEYITKVFVAFQAFLRGMVMYTSKD
RYFYFGKLDGQISSAYPSQEGQVLVGIYGQYQLLGIKSIGFEWNYPLEEPTTEPPVNLTYSANSPVGR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145252
ORF Size 624 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145252.2, NP_660295.2
RefSeq Size 846 bp
RefSeq ORF 519 bp
Locus ID 124220
UniProt ID Q96DA0
Cytogenetics 16p13.3
MW 22.7 kDa
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Documents
Resources
"NM_145252" in other vectors (7)
SKU Description Size Price
RC202244L1 Lenti ORF clone of Human zymogen granule protein 16 homolog B (rat) (ZG16B), Myc-DDK-tagged 10 ug
$600.00
RC202244L2 Lenti ORF clone of Human zymogen granule protein 16 homolog B (rat) (ZG16B), mGFP tagged 10 ug
$600.00
RC202244L3 Lenti ORF clone of Human zymogen granule protein 16 homolog B (rat) (ZG16B), Myc-DDK-tagged 10 ug
$600.00
RC202244L4 Lenti ORF clone of Human zymogen granule protein 16 homolog B (rat) (ZG16B), mGFP tagged 10 ug
$600.00
RG202244 ZG16B (tGFP-tagged) - Human zymogen granule protein 16 homolog B (rat) (ZG16B) 10 ug
$489.00 $500.00
SC100127 ZG16B (untagged)-Human zymogen granule protein 16 homolog B (rat) (ZG16B) 10 ug
$300.00
SC317308 ZG16B (untagged)-Human zymogen granule protein 16 homolog B (rat) (ZG16B) 10 ug
$330.00

Plasmid Map for RC202244

Download sequence for

Cloning scheme for RC202244

Circular map for RC202244

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