TRIM21 (NM_003141) Human Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TRIM21 |
| Synonyms | RNF81; Ro/SSA; RO52; SSA; SSA1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003141 |
| ORF Size | 1425 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003141.4 |
| RefSeq Size | 1946 bp |
| RefSeq ORF | 1428 bp |
| Locus ID | 6737 |
| UniProt ID | P19474 |
| Cytogenetics | 11p15.4 |
| Domains | PRY, RING, SPRY, zf-B_box |
| Protein Families | Druggable Genome |
| Protein Pathways | Systemic lupus erythematosus |
| MW | 54.2 kDa |
| Summary | This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003141" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202088L1 | Lenti ORF clone of Human tripartite motif containing 21 (TRIM21), Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC202088L2 | Lenti ORF clone of Human tripartite motif containing 21 (TRIM21), mGFP tagged | 10 ug |
$986.00
|
|
| RC202088L3 | Lenti ORF clone of Human tripartite motif containing 21 (TRIM21), Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC202088L4 | Lenti ORF clone of Human tripartite motif containing 21 (TRIM21), mGFP tagged | 10 ug |
$986.00
|
|
| RG202088 | TRIM21 (tGFP-tagged) - Human tripartite motif containing 21 (TRIM21) | 10 ug |
$886.00
|
|
| SC118185 | TRIM21 (untagged)-Human tripartite motif containing 21 (TRIM21) | 10 ug |
$686.00
|
|
Citations
FAT10 inhibits TRIM21 to down-regulate antiviral type-I interferon secretion
Saxena, Inholz, Basler et al
Life Sci Alliance (2024) 7 (9) DOI: 10.26508/lsa.202402786
Saxena, Inholz, Basler et al
Life Sci Alliance (2024) 7 (9) DOI: 10.26508/lsa.202402786
Cited by 5
AI Summary: OriGene’s pCMV6-Myc-DDK-TRIM21 plasmid (RC202088) was used in this study to express TRIM21 in HEK293 cells, enabling the investigation of FAT10-mediated regulation of TRIM21 and its impact on antiviral type-I interferon secretion.
Snippet: residium Freiburg of the State of Baden-Württemberg, Germany (G-24-008 and G-18/72). DNA constructs Plasmids used for the transient transfection of HEK293 cells were pCMV6-Myc-DDK-TRIM21 (RC202088;Origene), pcDNA3.1-HA-FAT10 (Hipp et al, 2004), pcDNA3.1-HA-FAT10AV (Aichem et al, 2010), pcDNA3.1-HA-UBA6 (Aichem et al, 2010), pcDNA-3.1-His/-A-USE1, and its active site cysteine mutant pcDNA-3.1-Hi
Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators
Nikolaou, Godbersen, Manoharan et al
JCI Insight (2023) 8 (21) DOI: 10.1172/jci.insight.164694
Nikolaou, Godbersen, Manoharan et al
JCI Insight (2023) 8 (21) DOI: 10.1172/jci.insight.164694
Cited by 22
TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation
Li, Wang, Yang et al
Nat Commun (2020) 11 (1), 6316 DOI: 10.1038/s41467-020-20107-1
Li, Wang, Yang et al
Nat Commun (2020) 11 (1), 6316 DOI: 10.1038/s41467-020-20107-1
Cited by 62
AI Summary: OriGene products, specifically the TRIB3 shRNA (OriGene, TL313888) and TRIB3 expression plasmid (OriGene, RC210307), were used in this study to manipulate TRIB3 levels in lymphoma cells, enabling the authors to investigate the role of TRIB3 in regulating MYC stability and lymphoma progression.
Snippet: its interactions with intracellular signaling and functional proteins16–19. TRIB3 is emerging as a potential therapeutic target for cancer because abrogating its expression dramatically reduces tumorigenesis and cancer progression17–22. Interestingly, the expression of TRIB2, another member of the pseudokinase family, is elevated in T-cell acute lymphoblastic leukemia (T-ALL)23, and TRIB2 has
SFTS virus NSs interacts with TRIM21 to activate the p62-Keap1-Nrf2 pathway
Choi, Jiang, Shin et al
J. Virol. (2019)
Choi, Jiang, Shin et al
J. Virol. (2019)
AI Summary: OriGene products, specifically the cDNAs for human TRIM21, p62, Keap1, ABIN2, and TBK1, were used in this study to generate expression constructs that enabled the investigation of how SFTSV NSs protein interacts with TRIM21 to activate the p62-Keap1-Nrf2 antioxidant pathway.
Snippet: … cDNAs for human TRIM21, p62, Keap1, ABIN2, and TBK1 were obtained from Addgene 348 and Origene. … All NSs-expression plasmids contain a C-terminal 3xFlag, V5, or 3xFlag/GFP 351 tags and N-terminal GST tag, TRIM21-expression plasmid has a C-terminal V5 tag, p62- 352 expression plasmids have C-terminal Myc or HA tags, Keap1-expression plasmid contains a C353 terminal Flag tag, ABIN2-expression plasmid contains a C-terminal V5 tag, and TBK1- 354 expression plasmid contains a C-terminal Flag tag. …
Showing 1-4 of 4 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
