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MLC1SA (MYL6B) (NM_002475) Human Tagged ORF Clone

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SKU
RC202076
MYL6B (Myc-DDK-tagged)-Human myosin, light chain 6B, alkali, smooth muscle and non-muscle (MYL6B), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MLC1SA
Synonyms MLC1SA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202076 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCCCAAGAAGGATGTTCCCGTGAAGAAACCAGCAGGGCCCTCCATCTCCAAACCTGCTGCTAAGC
CAGCAGCAGCAGGGGCTCCTCCAGCCAAGACCAAAGCTGAGCCAGCTGTCCCCCAGGCCCCTCAGAAAAC
CCAGGAGCCTCCAGTCGATCTCTCCAAAGTGGTGATCGAGTTTAACAAGGACCAGCTGGAGGAGTTCAAG
GAGGCCTTCGAGCTGTTTGACCGAGTGGGGGATGGCAAGATCCTGTACAGCCAGTGTGGGGACGTGATGA
GGGCCCTGGGCCAGAACCCCACCAACGCCGAGGTGCTCAAGGTCCTGGGGAACCCCAAGAGTGATGAGCT
GAAGTCGCGGCGTGTGGACTTTGAGACTTTCCTGCCCATGCTCCAGGCAGTGGCCAAGAACCGAGGCCAA
GGCACATATGAGGACTACTTGGAGGGGTTTCGTGTGTTTGACAAGGAGGGGAACGGCAAAGTCATGGGAG
CAGAGCTCAGACATGTTCTCACCACCCTTGGAGAGAAGATGACTGAGGAGGAGGTGGAGACCGTTCTGGC
AGGACACGAGGACAGCAACGGCTGCATCAACTACGAGGCCTTCTTGAAACACATCCTAAGCGTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202076 protein sequence
Red=Cloning site Green=Tags(s)

MPPKKDVPVKKPAGPSISKPAAKPAAAGAPPAKTKAEPAVPQAPQKTQEPPVDLSKVVIEFNKDQLEEFK
EAFELFDRVGDGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDELKSRRVDFETFLPMLQAVAKNRGQ
GTYEDYLEGFRVFDKEGNGKVMGAELRHVLTTLGEKMTEEEVETVLAGHEDSNGCINYEAFLKHILSV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002475
ORF Size 624 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002475.5
RefSeq Size 1008 bp
RefSeq ORF 627 bp
Locus ID 140465
UniProt ID P14649, A0A024RB31
Cytogenetics 12q13.2
Domains EFh
Protein Pathways Vascular smooth muscle contraction
MW 22.8 kDa
Summary Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in both slow-twitch skeletal muscle and in nonmuscle tissue. Alternative splicing results in multiple transcript variants. provided by RefSeq, Dec 2010
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"NM_002475" in other vectors (4)
SKU Description Size Price
RC202076L3 Lenti ORF clone of Human myosin, light chain 6B, alkali, smooth muscle and non-muscle (MYL6B), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC202076L4 Lenti ORF clone of Human myosin, light chain 6B, alkali, smooth muscle and non-muscle (MYL6B), transcript variant 2, mGFP tagged 10 ug
$600.00
RG202076 MYL6B (tGFP-tagged) - Human myosin, light chain 6B, alkali, smooth muscle and non-muscle (MYL6B), transcript variant 2 10 ug
$500.00
SC118607 MYL6B (untagged)-Human myosin, light chain 6B, alkali, smooth muscle and non-muscle (MYL6B), transcript variant 2 10 ug
$300.00

Plasmid Map for RC202076

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202076
Circular map for RC202076

file-alt Citations

MYL6B, a myosin light chain, promotes MDM2-mediated p53 degradation and drives HCC development
Xie, Wang, Liao et al
J. Exp. Clin. Cancer Res. (2018) 37 (1), 28 DOI: 10.1186/s13046-018-0693-7
AI Summary: OriGene’s Myc-DDK-tagged Human MYL6B ORF (cloned into pCMV6) was used in this study to investigate the role of MYL6B in promoting MDM2-mediated p53 degradation and hepatocellular carcinoma development.
Snippet: LentiCRISPRv2 following provider's guide. Myc-DDK-tagged Human MYL6B ORF sequence was cloned into pCMV6 (OriGene). 3FLAG-tagged MYL6B ORF sequence was cloned into pcDNA3. Rabbit polyclone antibodies Cached
FigureFigureFigure
Showing 1-1 of 1 papers.
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