STOM (NM_004099) Human Tagged ORF Clone

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SKU
RC202060
STOM (Myc-DDK-tagged)-Human stomatin (STOM), transcript variant 1
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol STOM
Synonyms BND7; EPB7; EPB72
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202060 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGAAGCGGCACACACGGGACTCCGAAGCCCAGCGGCTCCCCGACTCCTTCAAGGACAGCCCCA
GTAAGGGCCTTGGACCTTGCGGATGGATTTTGGTGGCGTTCTCATTCTTATTCACCGTTATAACTTTCCC
AATCTCAATATGGATGTGCATAAAGATTATAAAAGAGTATGAAAGAGCCATCATCTTTAGATTGGGTCGC
ATTTTACAAGGAGGAGCCAAAGGACCTGGTTTGTTTTTTATTCTGCCATGCACTGACAGCTTCATCAAAG
TGGACATGAGAACTATTTCATTTGATATTCCTCCTCAGGAGATCCTCACAAAGGATTCAGTGACAATTAG
CGTGGATGGTGTGGTCTATTACCGCGTTCAGAATGCAACCCTGGCTGTGGCAAATATCACCAACGCTGAC
TCAGCAACCCGTCTTTTGGCACAAACTACTCTGAGGAATGTTCTGGGCACCAAGAATCTTTCTCAGATCC
TCTCTGACAGAGAAGAAATTGCACACAACATGCAGTCTACTCTGGATGATGCCACTGATGCCTGGGGAAT
AAAGGTGGAGCGTGTGGAAATTAAGGATGTGAAACTACCTGTGCAGCTCCAGAGAGCTATGGCTGCAGAA
GCAGAAGCGTCCCGCGAGGCCCGCGCCAAGGTTATTGCAGCCGAAGGAGAAATGAATGCATCCAGGGCTC
TGAAAGAAGCCTCCATGGTCATCACTGAATATCCTGCAGCCCTTCAGCTCCGATACCTGCAGACACTGAC
CACCATTGCTGCTGAGAAAAACTCAACAATTGTCTTCCCTCTGCCCATAGATATGCTGCAAGGAATCATA
GGGGCAAAACACAGCCATCTAGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202060 protein sequence
Red=Cloning site Green=Tags(s)

MAEKRHTRDSEAQRLPDSFKDSPSKGLGPCGWILVAFSFLFTVITFPISIWMCIKIIKEYERAIIFRLGR
ILQGGAKGPGLFFILPCTDSFIKVDMRTISFDIPPQEILTKDSVTISVDGVVYYRVQNATLAVANITNAD
SATRLLAQTTLRNVLGTKNLSQILSDREEIAHNMQSTLDDATDAWGIKVERVEIKDVKLPVQLQRAMAAE
AEASREARAKVIAAEGEMNASRALKEASMVITEYPAALQLRYLQTLTTIAAEKNSTIVFPLPIDMLQGII
GAKHSHLG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004099
ORF Size 864 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004099.2
RefSeq Size 3198 bp
RefSeq ORF 867 bp
Locus ID 2040
UniProt ID P27105, A0A024R882
Cytogenetics 9q33.2
Domains Band_7
Protein Families Transmembrane
MW 31.8 kDa
Summary This gene encodes a member of a highly conserved family of integral membrane proteins. The encoded protein localizes to the cell membrane of red blood cells and other cell types, where it may regulate ion channels and transporters. Loss of localization of the encoded protein is associated with hereditary stomatocytosis, a form of hemolytic anemia. There is a pseudogene for this gene on chromosome 6. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2012
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Documents
Resources
"NM_004099" in other vectors (4)
SKU Description Size Price
RC202060L3 Lenti ORF clone of Human stomatin (STOM), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC202060L4 Lenti ORF clone of Human stomatin (STOM), transcript variant 1, mGFP tagged 10 ug
$600.00
RG202060 STOM (tGFP-tagged) - Human stomatin (STOM), transcript variant 1 10 ug
$500.00
SC117590 STOM (untagged)-Human stomatin (STOM), transcript variant 1 10 ug
$300.00

Plasmid Map for RC202060

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Cloning scheme for RC202060

Circular map for RC202060

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