Phospholipase D2 (PLD2) (NM_002663) Human Tagged ORF Clone
$1,281.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Phospholipase D2 |
| Synonyms | PLD1C |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002663 |
| ORF Size | 2799 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002663.2, NP_002654.2 |
| RefSeq Size | 3475 bp |
| RefSeq ORF | 2802 bp |
| Locus ID | 5338 |
| UniProt ID | O14939 |
| Cytogenetics | 17p13.2 |
| Domains | PH, PLDc, PX |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis, Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Metabolic pathways |
| MW | 105.8 kDa |
| Summary | The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.provided by RefSeq, Jul 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002663" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202042L1 | Lenti-ORF clone of PLD2 (Myc-DDK-tagged)-Human phospholipase D2 (PLD2) | 10 ug |
$1,581.00
|
|
| RC202042L2 | Lenti-ORF clone of PLD2 (mGFP-tagged)-Human phospholipase D2 (PLD2) | 10 ug |
$1,581.00
|
|
| RC202042L3 | Lenti-ORF clone of PLD2 (Myc-DDK-tagged)-Human phospholipase D2 (PLD2) | 10 ug |
$1,581.00
|
|
| RC202042L4 | Lenti-ORF clone of PLD2 (mGFP-tagged)-Human phospholipase D2 (PLD2) | 10 ug |
$1,581.00
|
|
| RG202042 | PLD2 (tGFP-tagged) - Human phospholipase D2 (PLD2) | 10 ug |
$1,481.00
|
|
| SC118498 | PLD2 (untagged)-Human phospholipase D2 (PLD2) | 10 ug |
$855.00
|
|
| SC324003 | PLD2 (untagged)-Human phospholipase D2 (PLD2) | 10 ug |
$855.00
|
|
Citations
Upregulated phospholipase D2 expression and activity is related to the metastatic properties of melanoma
Perez-Valle, Ochoa, Shah et al
Oncol Lett (2022) 23 (5), 140 DOI: 10.3892/ol.2022.13260
Perez-Valle, Ochoa, Shah et al
Oncol Lett (2022) 23 (5), 140 DOI: 10.3892/ol.2022.13260
Cited by 4
AI Summary: In this study, OriGene’s PLD2 overexpression plasmid (cat. no. RC202042) was used to investigate the role of PLD2 in melanoma cells, demonstrating that increased PLD2 expression enhances the metastatic properties of melanoma.
Snippet: anoma cells were seeded in 6-well plates and grown until 60–70% confluence. They were then transfected by incubation for 48 h with a transfection mixture of 1 µg of plasmid DNA (cat. no. RC202042;Origene Technologies, Inc.), TransIT −2020 transfection reagent (Mirus Bio, LLC) and Opti-MEM (Thermo Fisher Scientific, Inc.). Control cells were transfected with empty plasmid (pc-DNA3.1-myc
Showing 1-1 of 1 papers.
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