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CCNDBP1 (NM_012142) Human Tagged ORF Clone

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SKU
RC202006
CCNDBP1 (Myc-DDK-tagged)-Human cyclin D-type binding-protein 1 (CCNDBP1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "CCNDBP1 or NM_012142"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCNDBP1
Synonyms DIP1; GCIP; HHM
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202006 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAGCGCAACTGCACCTGCAGCCGCAGTCCCCACCCTGGCTTCGCCTTTGGAGCAGCTCCGGCACT
TGGCGGAGGAGCTGCGGTTGCTCCTGCCTCGAGTGCGGGTCGGCGAAGCCCAGGAGACCACCGAGGAGTT
TAATCGAGAGATGTTCTGGAGAAGACTCAATGAGGCAGCTGTGACTGTGTCAAGGGAAGCCACGACTCTG
ACCATAGTCTTCTCTCAGCTTCCACTGCCGTCTCCACAGGAAACCCAGAAGTTCTGTGAACAAGTCCATG
CTGCCATCAAGGCATTTATTGCAGTGTACTATTTGCTTCCAAAGGATCAGGGGATCACCCTGAGAAAGCT
GGTACGGGGCGCCACCCTGGACATCGTGGATGGCATGGCTCAGCTCATGGAAGTACTTTCCGTCACTCCA
ACTCAGAGCCCTGAGAACAATGACCTTATTTCCTACAACAGTGTCTGGGTTGCGTGCCAGCAGATGCCTC
AGATACCAAGAGATAACAAAGCTGCAGCTCTTTTGATGCTGACCAAGAATGTGGATTTTGTGAAGGATGC
ACATGAAGAAATGGAGCAGGCTGTGGAAGAATGTGACCCTTACTCTGGCCTCTTGAATGATACTGAGGAG
AACAACTCTGACAACCACAATCATGAGGATGATGTGTTGGGGTTTCCCAGCAATCAGGACTTGTATTGGT
CAGAGGACGATCAAGAGCTCATAATCCCATGCCTTGCGCTGGTGAGAGCATCCAAAGCCTGCCTGAAGAA
AATTCGGATGTTAGTGGCAGAGAATGGGAAGAAGGATCAGGTGGCACAGCTGGATGACATTGTGGATATT
TCTGATGAAATCAGCCCTAGTGTGGATGATTTGGCTCTGAGCATATATCCACCTATGTGTCACCTGACCG
TGCGAATCAATTCTGCGAAACTTGTATCTGTTTTAAAGAAGGCACTTGAAATTACAAAAGCAAGTCATGT
GACCCCTCAGCCAGAAGATAGTTGGATCCCTTTACTTATTAATGCCATTGATCATTGCATGAATAGAATC
AAGGAGCTCACTCAGAGTGAACTTGAATTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202006 protein sequence
Red=Cloning site Green=Tags(s)

MASATAPAAAVPTLASPLEQLRHLAEELRLLLPRVRVGEAQETTEEFNREMFWRRLNEAAVTVSREATTL
TIVFSQLPLPSPQETQKFCEQVHAAIKAFIAVYYLLPKDQGITLRKLVRGATLDIVDGMAQLMEVLSVTP
TQSPENNDLISYNSVWVACQQMPQIPRDNKAAALLMLTKNVDFVKDAHEEMEQAVEECDPYSGLLNDTEE
NNSDNHNHEDDVLGFPSNQDLYWSEDDQELIIPCLALVRASKACLKKIRMLVAENGKKDQVAQLDDIVDI
SDEISPSVDDLALSIYPPMCHLTVRINSAKLVSVLKKALEITKASHVTPQPEDSWIPLLINAIDHCMNRI
KELTQSELEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012142
ORF Size 1080 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012142.5
RefSeq Size 3669 bp
RefSeq ORF 1083 bp
Locus ID 23582
UniProt ID O95273
Cytogenetics 15q15.2
MW 40.3 kDa
Summary This gene was identified by the interaction of its gene product with Grap2, a leukocyte-specific adaptor protein important for immune cell signaling. The protein encoded by this gene was shown to interact with cyclin D. Transfection of this gene in cells was reported to reduce the phosphorylation of Rb gene product by cyclin D-dependent protein kinase, and inhibit E2F1-mediated transcription activity. This protein was also found to interact with helix-loop-helix protein E12 and is thought to be a negative regulator of liver-specific gene expression. Several alternatively spliced variants have been found for this gene. provided by RefSeq, Apr 2009
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"NM_012142" in other vectors (5)
SKU Description Size Price
RC202006L3 Lenti ORF clone of Human cyclin D-type binding-protein 1 (CCNDBP1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC202006L4 Lenti ORF clone of Human cyclin D-type binding-protein 1 (CCNDBP1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG202006 CCNDBP1 (tGFP-tagged) - Human cyclin D-type binding-protein 1 (CCNDBP1), transcript variant 1 10 ug
$489.00 $657.00
SC110316 CCNDBP1 (untagged)-Human cyclin D-type binding-protein 1 (CCNDBP1), transcript variant 1 10 ug
$457.00
SC322349 CCNDBP1 (untagged)-Human cyclin D-type binding-protein 1 (CCNDBP1), transcript variant 1 10 ug
$457.00

Plasmid Map for RC202006

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202006
Circular map for RC202006

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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