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C6orf47 (NM_021184) Human Tagged ORF Clone

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SKU
RC201942
C6orf47 (Myc-DDK-tagged)-Human chromosome 6 open reading frame 47 (C6orf47)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol C6orf47
Synonyms D6S53E; G4; NG34
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201942 representing NM_021184
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCTGCGACGGCTTGGTGGCTGGCTACCTCGCCCTTGGGGCCGCCGGAAACCAATGAGGCCTGACC
CGCCTTACCCAGAACCCAGACGGGTGGACAGCTCCTCGGAGAATTCAGGAAGTGACTGGGATAGTGCCCC
AGAAACCATGGAAGATGTGGGGCATCCCAAGACTAAGGACTCGGGGGCATTGAGGGTTTCTAGGGCTGCT
TCCGAACCAAGCAAGGAGGAGCCCCAAGTTGAGCAGCTAGGGAGCAAAAGAATGGATTCCCTCAAGTGGG
ACCAGCCTATCTCTAGCACTCAAGAGTCTGGGAGACTGGAGGCTGGAGGGGCCAGTCCCAAACTCAGATG
GGATCATGTGGATTCAGGTGGCACCAGGAGACCAGGGGTGTCCCCTGAAGGGGGACTGAGCGTCCCTGGG
CCAGGAGCCCCATTGGAGAAACCTGGTAGGCGTGAGAAGCTGTTGGGCTGGCTGCGGGGGGAACCAGGAG
CTCCCTCCCGGTACTTGGGGGGCCCAGAAGAGTGTCTGCAGATCTCCACCAACCTGACCCTGCATCTTCT
GGAGCTGCTGGCCTCTGCCCTGCTGGCCCTGTGCTCACGACCACTGCGGGCAGCCTTGGACACACTGGGC
CTGCGTGGACCGCTGGGCCTCTGGCTACATGGCCTACTGTCCTTCCTGGCTGCCCTGCATGGGCTCCATG
CTGTTCTGAGCCTACTTACTGCCCACCCTTTGCACTTCGCCTGCCTCTTTGGTCTCCTGCAGGCCTTGGT
GCTGGCTGTCAGCCTCCGGGAGCCCAATGGGGATGAGGCGGCCACTGACTGGGAGAGTGAGGGGTTGGAG
AGGGAAGGTGAGGAGCAGAGGGGAGACCCGGGAAAGGGGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201942 representing NM_021184
Red=Cloning site Green=Tags(s)

MFLRRLGGWLPRPWGRRKPMRPDPPYPEPRRVDSSSENSGSDWDSAPETMEDVGHPKTKDSGALRVSRAA
SEPSKEEPQVEQLGSKRMDSLKWDQPISSTQESGRLEAGGASPKLRWDHVDSGGTRRPGVSPEGGLSVPG
PGAPLEKPGRREKLLGWLRGEPGAPSRYLGGPEECLQISTNLTLHLLELLASALLALCSRPLRAALDTLG
LRGPLGLWLHGLLSFLAALHGLHAVLSLLTAHPLHFACLFGLLQALVLAVSLREPNGDEAATDWESEGLE
REGEEQRGDPGKGL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021184
ORF Size 882 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021184.2
RefSeq Size 2475 bp
RefSeq ORF 885 bp
Locus ID 57827
UniProt ID O95873, A0A1U9X7F2, Q9UMP7
Cytogenetics 6p21.33
MW 31.5 kDa
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Documents
Resources
"NM_021184" in other vectors (4)
SKU Description Size Price
RC201942L3 Lenti ORF clone of Human chromosome 6 open reading frame 47 (C6orf47), Myc-DDK-tagged 10 ug
$600.00
RC201942L4 Lenti ORF clone of Human chromosome 6 open reading frame 47 (C6orf47), mGFP tagged 10 ug
$600.00
RG201942 C6orf47 (tGFP-tagged) - Human chromosome 6 open reading frame 47 (C6orf47) 10 ug
$500.00
SC113003 C6orf47 (untagged)-Human chromosome 6 open reading frame 47 (C6orf47) 10 ug
$300.00

Plasmid Map for RC201942

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201942
Circular map for RC201942

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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