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SLC17A5 (NM_012434) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC201915
SLC17A5 (Myc-DDK-tagged)-Human solute carrier family 17 (anion/sugar transporter), member 5 (SLC17A5)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC17A5
Synonyms AST; ISSD; NSD; SD; SIALIN; SIASD; SLD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201915 representing NM_012434
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGTCTCCGGTTCGAGACCTGGCCCGGAACGATGGCGAGGAGAGCACGGACCGCACGCCTCTTCTAC
CGGGCGCCCCACGGGCCGAAGCCGCTCCAGTGTGCTGCTCTGCTCGTTACAACTTAGCAATTTTGGCCTT
TTTTGGTTTCTTCATTGTGTATGCATTACGTGTGAATCTGAGTGTTGCGTTAGTGGATATGGTAGATTCA
AATACAACTTTAGAAGATAATAGAACTTCCAAGGCATGTCCAGAGCATTCTGCTCCCATAAAAGTTCATC
ATAATCAAACGGGTAAGAAGTACCAATGGGATGCAGAAACTCAAGGATGGATTCTCGGTTCCTTTTTTTA
TGGCTACATCATCACACAGATTCCTGGAGGATATGTTGCCAGCAAAATAGGGGGGAAAATGCTGCTAGGA
TTTGGGATCCTTGGCACTGCTGTCCTCACCCTGTTCACTCCCATTGCTGCAGATTTAGGAGTTGGACCAC
TCATTGTACTCAGAGCACTAGAAGGACTAGGAGAGGGTGTTACATTTCCAGCCATGCATGCCATGTGGTC
TTCTTGGGCTCCCCCTCTTGAAAGAAGCAAACTTCTTAGCATTTCATATGCAGGAGCACAGCTTGGGACA
GTAATTTCTCTTCCTCTTTCTGGAATAATTTGCTACTATATGAATTGGACTTATGTCTTCTACTTTTTTG
GTACTATTGGAATATTTTGGTTTCTTTTGTGGATCTGGTTAGTTAGTGACACACCACAAAAACACAAGAG
AATTTCCCATTATGAAAAGGAATACATTCTTTCATCATTAAGAAATCAGCTTTCTTCACAGAAGTCAGTG
CCGTGGGTACCCATTTTAAAATCCCTGCCACTTTGGGCTATCGTAGTTGCACACTTTTCTTACAACTGGA
CTTTTTATACTTTATTGACATTATTGCCTACTTATATGAAGGAGATCCTAAGGTTCAATGTTCAAGAGAA
TGGGTTTTTATCTTCATTGCCTTATTTAGGCTCTTGGTTATGTATGATCCTGTCTGGTCAAGCTGCTGAC
AATTTAAGGGCAAAATGGAATTTTTCAACTTTATGTGTTCGCAGAATTTTTAGCCTTATAGGAATGATTG
GACCTGCAGTATTCCTGGTAGCTGCTGGCTTCATTGGCTGTGATTATTCTTTGGCCGTTGCTTTCCTAAC
TATATCAACAACACTGGGAGGCTTTTGCTCTTCTGGATTTAGCATCAACCATCTGGATATTGCTCCTTCG
TATGCTGGTATCCTCCTGGGCATCACAAATACATTTGCCACTATTCCAGGAATGGTTGGGCCCGTCATTG
CTAAAAGTCTGACCCCTGATAACACTGTTGGAGAATGGCAAACCGTGTTCTATATTGCTGCTGCTATTAA
TGTTTTTGGTGCCATTTTCTTTACACTATTCGCCAAAGGTGAAGTACAAAACTGGGCTCTCAATGATCAC
CATGGACACAGACAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201915 representing NM_012434
Red=Cloning site Green=Tags(s)

MRSPVRDLARNDGEESTDRTPLLPGAPRAEAAPVCCSARYNLAILAFFGFFIVYALRVNLSVALVDMVDS
NTTLEDNRTSKACPEHSAPIKVHHNQTGKKYQWDAETQGWILGSFFYGYIITQIPGGYVASKIGGKMLLG
FGILGTAVLTLFTPIAADLGVGPLIVLRALEGLGEGVTFPAMHAMWSSWAPPLERSKLLSISYAGAQLGT
VISLPLSGIICYYMNWTYVFYFFGTIGIFWFLLWIWLVSDTPQKHKRISHYEKEYILSSLRNQLSSQKSV
PWVPILKSLPLWAIVVAHFSYNWTFYTLLTLLPTYMKEILRFNVQENGFLSSLPYLGSWLCMILSGQAAD
NLRAKWNFSTLCVRRIFSLIGMIGPAVFLVAAGFIGCDYSLAVAFLTISTTLGGFCSSGFSINHLDIAPS
YAGILLGITNTFATIPGMVGPVIAKSLTPDNTVGEWQTVFYIAAAINVFGAIFFTLFAKGEVQNWALNDH
HGHRH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012434
ORF Size 1485 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012434.3, NP_036566.1
RefSeq Size 3292 bp
RefSeq ORF 1488 bp
Locus ID 26503
UniProt ID Q9NRA2
Cytogenetics 6q13
Domains sugar_tr
Protein Families Transmembrane
Protein Pathways Lysosome
MW 54.5 kDa
Summary This gene encodes a membrane transporter that exports free sialic acids that have been cleaved off of cell surface lipids and proteins from lysosomes. Mutations in this gene cause sialic acid storage diseases, including infantile sialic acid storage disorder and and Salla disease, an adult form. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_012434" in other vectors (7)
SKU Description Size Price
RC201915L1 Lenti ORF clone of Human solute carrier family 17 (anion/sugar transporter), member 5 (SLC17A5), Myc-DDK-tagged 10 ug
$757.00
RC201915L2 Lenti ORF clone of Human solute carrier family 17 (anion/sugar transporter), member 5 (SLC17A5), mGFP tagged 10 ug
$757.00
RC201915L3 Lenti ORF clone of Human solute carrier family 17 (anion/sugar transporter), member 5 (SLC17A5), Myc-DDK-tagged 10 ug
$757.00
RC201915L4 Lenti ORF clone of Human solute carrier family 17 (anion/sugar transporter), member 5 (SLC17A5), mGFP tagged 10 ug
$757.00
RG201915 SLC17A5 (tGFP-tagged) - Human solute carrier family 17 (anion/sugar transporter), member 5 (SLC17A5) 10 ug
$657.00
SC111234 SLC17A5 (untagged)-Human solute carrier family 17 (anion/sugar transporter), member 5 (SLC17A5) 10 ug
$457.00
SC324122 SLC17A5 (untagged)-Human solute carrier family 17 (anion/sugar transporter), member 5 (SLC17A5) 10 ug
$457.00

Plasmid Map for RC201915

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201915
Circular map for RC201915

file-alt Citations

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