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RBM14 (NM_006328) Human Tagged ORF Clone

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SKU
RC201739
RBM14 (Myc-DDK-tagged)-Human RNA binding motif protein 14 (RBM14), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $613.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RBM14
Synonyms COAA; PSP2; SIP; SYTIP1; TMEM137
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201739 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGATATTCGTGGGCAACGTCGACGGGGCGGATACGACTCCGGAGGAGCTGGCAGCCCTCTTTGCGC
CCTACGGCACGGTCATGAGCTGCGCCGTCATGAAACAGTTCGCCTTCGTGCACATGCGCGAGAACGCGGG
CGCGCTGCGCGCCATCGAAGCCCTGCACGGCCACGAGCTGCGGCCGGGGCGCGCGCTCGTGGTGGAGATG
TCGCGCCCAAGGCCTCTTAATACTTGGAAGATTTTCGTGGGCAATGTGTCGGCTGCATGCACGAGCCAGG
AACTGCGCAGCCTCTTCGAGCGCCGCGGACGCGTCATCGAGTGTGACGTGGTGAAAGACTACGCGTTTGT
TCACATGGAGAAGGAAGCAGATGCCAAAGCCGCAATCGCGCAGCTCAACGGCAAAGAAGTGAAGGGCAAG
CGCATCAACGTGGAACTCTCCACCAAGGGTCAGAAGAAGGGGCCTGGCCTGGCTGTCCAGTCTGGGGACA
AGACCAAGAAACCAGGGGCTGGGGATACGGCCTTCCCTGGAACTGGTGGCTTCTCTGCCACCTTCGACTA
CCAGCAGGCTTTTGGCAACAGCACTGGTGGCTTTGATGGGCAAGCCCGTCAGCCCACACCACCCTTCTTT
GGTCGCGACCGCAGCCCTCTGCGCCGTTCACCTCCCCGAGCCTCTTATGTGGCTCCTCTGACGGCCCAGC
CAGCTACCTACCGGGCCCAGCCGTCCGTGTCACTGGGAGCTGCCTACAGGGCCCAGCCTTCTGCCTCTTT
GGGTGTTGGCTATCGGACTCAGCCCATGACAGCCCAGGCAGCCTCTTACCGCGCTCAGCCCTCTGTCTCC
CTTGGGGCACCATACAGGGGCCAGCTGGCTAGTCCTAGCTCCCAGTCTGCTGCAGCTTCTTCACTCGGCC
CATATGGTGGAGCCCAGCCCTCAGCCTCGGCCCTTTCCTCCTATGGGGGTCAGGCAGCTGCAGCTTCTTC
GCTCAACTCCTATGGGGCTCAGGGTTCCTCCCTTGCCTCCTATGGTAACCAGCCATCCTCTTACGGCGCC
CAGGCTGCCTCTTCCTATGGGGTTCGTGCAGCTGCTTCTTCCTACAACACCCAGGGAGCAGCTTCCTCCT
TAGGCTCCTACGGGGCTCAGGCAGCCTCCTATGGGGCCCAGTCTGCAGCCTCCTCACTAGCTTATGGAGC
CCAGGCAGCTTCATATAATGCCCAGCCCTCGGCCTCTTACAATGCCCAGTCTGCCCCATATGCTGCACAG
CAGGCTGCTTCCTACTCTTCCCAACCTGCTGCCTATGTGGCACAGCCAGCCACAGCTGCTGCCTATGCCA
GCCAGCCAGCAGCCTACGCCGCACAAGCCACTACCCCAATGGCTGGCTCCTATGGGGCCCAGCCGGTTGT
GCAGACCCAGCTGAATAGTTACGGGGCCCAAGCATCAATGGGCCTTTCAGGCTCCTATGGGGCTCAGTCG
GCTGCTGCGGCCACTGGCTCCTATGGTGCCGCAGCAGCCTACGGGGCCCAACCTTCTGCCACCCTGGCAG
CTCCTTACCGCACTCAGTCATCAGCCTCATTGGCTGCTTCCTATGCTGCCCAGCAGCATCCCCAGGCTGC
TGCCTCCTACCGCGGCCAGCCAGGCAATGCCTACGATGGGGCAGGTCAGCCGTCTGCAGCCTACCTGTCC
ATGTCCCAGGGGGCCGTTGCCAACGCCAACAGCACCCCGCCGCCCTATGAGCGTACCCGCCTCTCCCCAC
CCCGGGCCAGCTACGACGATCCCTACAAAAAGGCTGTCGCCATGTCGAAAAGGTATGGTTCCGACCGGCG
TTTAGCCGAGCTCTCTGATTACCGCCGTTTATCAGAGTCGCAGCTTTCGTTCCGCCGCTCGCCGACAAAG
TCCTCGCTGGATTACCGTCGCCTGCCCGATGCCCATTCCGATTACGCACGCTATTCGGGCTCCTATAATG
ATTACCTGCGGGCGGCTCAGATGCACTCTGGCTACCAGCGCCGCATG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201739 protein sequence
Red=Cloning site Green=Tags(s)

MKIFVGNVDGADTTPEELAALFAPYGTVMSCAVMKQFAFVHMRENAGALRAIEALHGHELRPGRALVVEM
SRPRPLNTWKIFVGNVSAACTSQELRSLFERRGRVIECDVVKDYAFVHMEKEADAKAAIAQLNGKEVKGK
RINVELSTKGQKKGPGLAVQSGDKTKKPGAGDTAFPGTGGFSATFDYQQAFGNSTGGFDGQARQPTPPFF
GRDRSPLRRSPPRASYVAPLTAQPATYRAQPSVSLGAAYRAQPSASLGVGYRTQPMTAQAASYRAQPSVS
LGAPYRGQLASPSSQSAAASSLGPYGGAQPSASALSSYGGQAAAASSLNSYGAQGSSLASYGNQPSSYGA
QAASSYGVRAAASSYNTQGAASSLGSYGAQAASYGAQSAASSLAYGAQAASYNAQPSASYNAQSAPYAAQ
QAASYSSQPAAYVAQPATAAAYASQPAAYAAQATTPMAGSYGAQPVVQTQLNSYGAQASMGLSGSYGAQS
AAAATGSYGAAAAYGAQPSATLAAPYRTQSSASLAASYAAQQHPQAAASYRGQPGNAYDGAGQPSAAYLS
MSQGAVANANSTPPPYERTRLSPPRASYDDPYKKAVAMSKRYGSDRRLAELSDYRRLSESQLSFRRSPTK
SSLDYRRLPDAHSDYARYSGSYNDYLRAAQMHSGYQRRM

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_006328
ORF Size 2007 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006328.4
RefSeq Size 5415 bp
RefSeq ORF 2010 bp
Locus ID 10432
UniProt ID Q96PK6, A0A0S2Z4Z0
Cytogenetics 11q13.2
Domains RRM
Protein Families Druggable Genome, Stem cell - Pluripotency, Transcription Factors
MW 69.5 kDa
Summary This gene encodes a ribonucleoprotein that functions as a general nuclear coactivator, and an RNA splicing modulator. This protein contains two RNA recognition motifs (RRM) at the N-terminus, and multiple hexapeptide repeat domain at the C-terminus that interacts with thyroid hormone receptor-binding protein (TRBP), and is required for transcription activation. Alternatively spliced transcript variants encoding different isoforms (with opposing effects on transcription) have been described for this gene. provided by RefSeq, Oct 2011
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"NM_006328" in other vectors (7)
SKU Description Size Price
RC201739L1 Lenti ORF clone of Human RNA binding motif protein 14 (RBM14), transcript variant 1, Myc-DDK-tagged 10 ug
$913.00
RC201739L2 Lenti ORF clone of Human RNA binding motif protein 14 (RBM14), transcript variant 1, mGFP tagged 10 ug
$913.00
RC201739L3 Lenti ORF clone of Human RNA binding motif protein 14 (RBM14), transcript variant 1, Myc-DDK-tagged 10 ug
$913.00
RC201739L4 Lenti ORF clone of Human RNA binding motif protein 14 (RBM14), transcript variant 1, mGFP tagged 10 ug
$913.00
RG201739 RBM14 (tGFP-tagged) - Human RNA binding motif protein 14 (RBM14), transcript variant 1 10 ug
$789.00 $813.00
SC108570 RBM14 (untagged)-Human RNA binding motif protein 14 (RBM14), transcript variant 1 10 ug
$614.00
SC324359 RBM14 (untagged)-Human RNA binding motif protein 14 (RBM14), transcript variant 1 10 ug
$614.00

Plasmid Map for RC201739

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201739
Circular map for RC201739

file-alt Citations

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