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WDR70 (NM_018034) Human Tagged ORF Clone

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SKU
RC201712
WDR70 (Myc-DDK-tagged)-Human WD repeat domain 70 (WDR70)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $609.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol WDR70
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201712 representing NM_018034
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGCTCTGGGCCCAGCGAAGTGACAGGCTCAGACGCGTCGGGACCGGACCCGCAGCTTGCGGTCA
CCATGGGCTTCACGGGGTTCGGTAAAAAAGCTCGCACATTTGACTTGGAAGCAATGTTTGAACAAACTCG
AAGGACAGCTGTGGAAAGAAGTCGCAAAACACTGGAAGCAAGAGAAAAAGAGGAAGAAATGAACAGAGAG
AAAGAATTAAGAAGACAAAATGAAGATATTGAGCCAACATCCTCAAGATCAAATGTGGTCAGAGATTGCT
CCAAATCATCTTCCAGGGATACGAGCAGCAGTGAAAGTGAACAGAGTTCTGACTCTTCTGATGATGAGTT
AATTGGCCCTCCTTTACCCCCTAAAATGGTAGGAAAACCAGTTAATTTTATGGAGGAAGATATCCTCGGT
CCTTTACCTCCACCTCTTAATGAAGAAGAAGAAGAAGCAGAGGAAGAAGAAGAGGAAGAGGAGGAAGAGG
AAAATCCTGTTCACAAGATTCCTGACTCGCATGAGATAACGCTGAAGCATGGCACTAAAACAGTGTCTGC
TTTGGGTCTGGATCCCTCAGGTGCCCGTTTGGTGACAGGAGGATATGACTATGATGTTAAGTTTTGGGAT
TTTGCTGGAATGGATGCTTCTTTTAAGGCATTTCGATCCCTTCAGCCCTGTGAGTGCCATCAGATCAAGT
CATTACAGTATAGTAACACAGGAGACATGATTCTTGTTGTATCTGGAAGCTCTCAGGCCAAGGTGATTGA
CAGAGATGGTTTTGAAGTAATGGAATGTATAAAAGGAGACCAGTATATTGTGGACATGGCCAACACCAAG
GGTCATACAGCAATGCTTCATACTGGCTCATGGCATCCCAAAATAAAGGGAGAATTTATGACTTGCTCAA
ATGATGCGACTGTGAGGACGTGGGAAGTTGAAAATCCAAAGAAGCAAAAAAGTGTGTTTAAACCACGGAC
GATGCAAGGCAAAAAAGTCATTCCCACTACGTGCACATATAGTAGAGATGGAAACCTCATAGCAGCTGCC
TGCCAGAATGGAAGCATACAGATCTGGGACCGAAATTTGACTGTTCATCCTAAGTTCCACTATAAACAGG
CTCATGACTCGGGCACAGACACTTCTTGCGTGACTTTTTCCTATGATGGTAATGTCCTTGCCTCTCGTGG
AGGTGACGATTCATTAAAATTATGGGACATCCGACAATTTAATAAACCACTTTTTTCAGCCTCGGGTCTT
CCCACCATGTTCCCAATGACTGACTGCTGTTTCAGTCCAGATGATAAGCTCATAGTCACTGGTACATCTA
TTCAAAGAGGATGTGGCAGCGGCAAACTTGTTTTCTTTGAGCGTAGGACTTTCCAAAGGGTGTATGAAAT
AGACATCACAGATGCGAGTGTTGTTCGCTGCCTGTGGCATCCAAAGCTGAACCAGATCATGGTTGGAACT
GGAAATGGATTGGCTAAAGTCTATTACGACCCCAACAAGAGTCAGAGGGGAGCAAAATTATGTGTGGTTA
AAACCCAGCGGAAGGCAAAACAAGCTGAGACTCTAACTCAGGACTACATCATCACCCCTCATGCCTTGCC
TATGTTCCGTGAGCCCCGCCAACGGAGTACAAGGAAACAGCTGGAGAAGGACAGACTGGATCCCCTGAAG
TCGCATAAACCTGAACCTCCTGTAGCAGGCCCAGGTCGTGGTGGCCGAGTTGGAACCCACGGGGGCACTC
TCTCTTCCTATATTGTGAAGAACATTGCTTTGGACAAGACCGATGACAGTAATCCTCGGGAAGCCATTTT
GCGTCATGCCAAAGCAGCAGAAGACAGCCCATATTGGGTTTCTCCAGCATATTCCAAGACTCAGCCCAAA
ACCATGTTTGCCCAAGTTGAATCTGATGATGAGGAAGCAAAGAATGAGCCAGAATGGAAAAAACGTAAAA
TT

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201712 representing NM_018034
Red=Cloning site Green=Tags(s)

MERSGPSEVTGSDASGPDPQLAVTMGFTGFGKKARTFDLEAMFEQTRRTAVERSRKTLEAREKEEEMNRE
KELRRQNEDIEPTSSRSNVVRDCSKSSSRDTSSSESEQSSDSSDDELIGPPLPPKMVGKPVNFMEEDILG
PLPPPLNEEEEEAEEEEEEEEEEENPVHKIPDSHEITLKHGTKTVSALGLDPSGARLVTGGYDYDVKFWD
FAGMDASFKAFRSLQPCECHQIKSLQYSNTGDMILVVSGSSQAKVIDRDGFEVMECIKGDQYIVDMANTK
GHTAMLHTGSWHPKIKGEFMTCSNDATVRTWEVENPKKQKSVFKPRTMQGKKVIPTTCTYSRDGNLIAAA
CQNGSIQIWDRNLTVHPKFHYKQAHDSGTDTSCVTFSYDGNVLASRGGDDSLKLWDIRQFNKPLFSASGL
PTMFPMTDCCFSPDDKLIVTGTSIQRGCGSGKLVFFERRTFQRVYEIDITDASVVRCLWHPKLNQIMVGT
GNGLAKVYYDPNKSQRGAKLCVVKTQRKAKQAETLTQDYIITPHALPMFREPRQRSTRKQLEKDRLDPLK
SHKPEPPVAGPGRGGRVGTHGGTLSSYIVKNIALDKTDDSNPREAILRHAKAAEDSPYWVSPAYSKTQPK
TMFAQVESDDEEAKNEPEWKKRKI

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_018034
ORF Size 1962 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018034.4
RefSeq Size 2247 bp
RefSeq ORF 1965 bp
Locus ID 55100
UniProt ID Q9NW82
Cytogenetics 5p13.2
Domains WD40
MW 73 kDa
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Documents
Resources
"NM_018034" in other vectors (6)
SKU Description Size Price
RC201712L1 Lenti-ORF clone of WDR70 (Myc-DDK-tagged)-Human WD repeat domain 70 (WDR70) 10 ug
$909.00
RC201712L2 Lenti-ORF clone of WDR70 (mGFP-tagged)-Human WD repeat domain 70 (WDR70) 10 ug
$909.00
RC201712L3 Lenti-ORF clone of WDR70 (Myc-DDK-tagged)-Human WD repeat domain 70 (WDR70) 10 ug
$909.00
RC201712L4 Lenti-ORF clone of WDR70 (mGFP-tagged)-Human WD repeat domain 70 (WDR70) 10 ug
$909.00
RG201712 WDR70 (tGFP-tagged) - Human WD repeat domain 70 (WDR70) 10 ug
$809.00
SC113818 WDR70 (untagged)-Human WD repeat domain 70 (WDR70) 10 ug
$610.00

Plasmid Map for RC201712

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201712
Circular map for RC201712

file-alt Citations

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