ELOVL6 (NM_024090) Human Tagged ORF Clone

  • TrueORF®
SKU
RC201703
ELOVL6 (Myc-DDK-tagged)-Human ELOVL fatty acid elongase 6 (ELOVL6), transcript variant 1
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ELOVL6
Synonyms FACE; FAE; LCE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201703 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACATGTCAGTGTTGACTTTACAAGAATATGAATTCGAAAAGCAGTTCAACGAGAATGAAGCCATCC
AATGGATGCAGGAAAACTGGAAGAAATCTTTCCTGTTTTCTGCTCTGTATGCTGCCTTTATATTCGGTGG
TCGGCACCTAATGAATAAACGAGCAAAGTTTGAACTGAGGAAGCCATTAGTGCTCTGGTCTCTGACCCTT
GCAGTCTTCAGTATATTCGGTGCTCTTCGAACTGGTGCTTATATGGTGTACATTTTGATGACCAAAGGCC
TGAAGCAGTCAGTTTGTGACCAGGGTTTTTACAATGGACCTGTCAGCAAATTCTGGGCTTATGCATTTGT
GCTAAGCAAAGCACCCGAACTAGGAGATACAATATTCATTATTCTGAGGAAGCAGAAGCTGATCTTCCTG
CACTGGTATCACCACATCACTGTGCTCCTGTACTCTTGGTACTCCTACAAAGACATGGTTGCCGGGGGAG
GTTGGTTCATGACTATGAACTATGGCGTGCACGCCGTGATGTACTCTTACTATGCCTTGCGGGCGGCAGG
TTTCCGAGTCTCCCGGAAGTTTGCCATGTTCATCACCTTGTCCCAGATCACTCAGATGCTGATGGGCTGT
GTGGTTAACTACCTGGTCTTCTGCTGGATGCAGCATGACCAGTGTCACTCTCACTTTCAGAACATCTTCT
GGTCCTCACTCATGTACCTCAGCTACCTTGTGCTCTTCTGCCATTTCTTCTTTGAGGCCTACATCGGCAA
AATGAGGAAAACAACGAAAGCTGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201703 protein sequence
Red=Cloning site Green=Tags(s)

MNMSVLTLQEYEFEKQFNENEAIQWMQENWKKSFLFSALYAAFIFGGRHLMNKRAKFELRKPLVLWSLTL
AVFSIFGALRTGAYMVYILMTKGLKQSVCDQGFYNGPVSKFWAYAFVLSKAPELGDTIFIILRKQKLIFL
HWYHHITVLLYSWYSYKDMVAGGGWFMTMNYGVHAVMYSYYALRAAGFRVSRKFAMFITLSQITQMLMGC
VVNYLVFCWMQHDQCHSHFQNIFWSSLMYLSYLVLFCHFFFEAYIGKMRKTTKAE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024090
ORF Size 795 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024090.3
RefSeq Size 3392 bp
RefSeq ORF 798 bp
Locus ID 79071
UniProt ID Q9H5J4
Cytogenetics 4q25
Domains ELO
Protein Families Transmembrane
Protein Pathways Biosynthesis of unsaturated fatty acids
MW 31.4 kDa
Summary Fatty acid elongases (EC 6.2.1.3), such as ELOVL6, use malonyl-CoA as a 2-carbon donor in the first and rate-limiting step of fatty acid elongation (Moon et al., 2001 PubMed 11567032).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_024090" in other vectors (6)
SKU Description Size Price
RC201703L1 Lenti ORF clone of Human ELOVL fatty acid elongase 6 (ELOVL6), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC201703L2 Lenti ORF clone of Human ELOVL fatty acid elongase 6 (ELOVL6), transcript variant 1, mGFP tagged 10 ug
$600.00
RC201703L3 Lenti ORF clone of Human ELOVL fatty acid elongase 6 (ELOVL6), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC201703L4 Lenti ORF clone of Human ELOVL fatty acid elongase 6 (ELOVL6), transcript variant 1, mGFP tagged 10 ug
$600.00
RG201703 ELOVL6 (tGFP-tagged) - Human ELOVL fatty acid elongase 6 (ELOVL6), transcript variant 1 10 ug
$489.00 $500.00
SC111410 ELOVL6 (untagged)-Human ELOVL fatty acid elongase 6 (ELOVL6), transcript variant 1 10 ug
$480.00

Plasmid Map for RC201703

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Cloning scheme for RC201703

Circular map for RC201703

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