IFITM3 (NM_021034) Human Tagged ORF Clone
$225.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | IFITM3 |
| Synonyms | 1-8U; DSPA2b; IP15 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_021034 |
| ORF Size | 399 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_021034.3 |
| RefSeq Size | 808 bp |
| RefSeq ORF | 402 bp |
| Locus ID | 10410 |
| UniProt ID | Q01628 |
| Cytogenetics | 11p15.5 |
| Domains | CD225 |
| Protein Families | Transmembrane |
| MW | 14.5 kDa |
| Summary | The protein encoded by this gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for this gene. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined. provided by RefSeq, May 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_021034" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201635L1 | Lenti ORF clone of Human interferon induced transmembrane protein 3 (IFITM3), Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC201635L2 | Lenti ORF clone of Human interferon induced transmembrane protein 3 (IFITM3), mGFP tagged | 10 ug |
$525.00
|
|
| RC201635L3 | Lenti ORF clone of Human interferon induced transmembrane protein 3 (IFITM3), Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC201635L4 | Lenti ORF clone of Human interferon induced transmembrane protein 3 (IFITM3), mGFP tagged | 10 ug |
$525.00
|
|
| RG201635 | IFITM3 (tGFP-tagged) - Human interferon induced transmembrane protein 3 (IFITM3) | 10 ug |
$425.00
|
|
| SC112616 | IFITM3 (untagged)-Human interferon induced transmembrane protein 3 (IFITM3) | 10 ug |
$225.00
|
|
Citations
IFITM3 promotes triple-negative breast cancer progression by regulating β-catenin localization
Kim, Yu, Park et al
Biochem Biophys Res Commun (2026) 831, 154335 DOI: 10.1016/j.bbrc.2026.154335
Kim, Yu, Park et al
Biochem Biophys Res Commun (2026) 831, 154335 DOI: 10.1016/j.bbrc.2026.154335
IFITM3-mediated activation of TRAF6/MAPK/AP-1 pathways induces acquired TKI resistance in clear cell renal cell carcinoma
Jeong, Park, Yoon et al
Investig Clin Urol (2024) 65 (1), 84-93 DOI: 10.4111/icu.20230294
Jeong, Park, Yoon et al
Investig Clin Urol (2024) 65 (1), 84-93 DOI: 10.4111/icu.20230294
Cited by 4
AI Summary: OriGene contributed to this study by providing the pCMV6-Entry vector containing the IFITM3 ORF construct (RC201635), which was used for ectopic overexpression of IFITM3 proteins to investigate their role in TKI resistance in clear cell renal cell carcinoma.
Snippet: For ectopic overexpression of IFITM3 proteins, a lentivirus containing the pCMV6-Entry vector cloned with IFITM3 ORF construct was purchased (RC201635, OriGene).
IFITM1 enhances nonenveloped viral RNA replication by facilitating cholesterol transport to the Golgi
Ishikawa-Sasaki, Murata, Sasaki
PLoS Pathog (2023) 19 (5), e1011383 DOI: 10.1371/journal.ppat.1011383
Ishikawa-Sasaki, Murata, Sasaki
PLoS Pathog (2023) 19 (5), e1011383 DOI: 10.1371/journal.ppat.1011383
Cited by 6
AI Summary: OriGene cDNA clones for IFITM1 (RC201617), IFITM2 (RC202067), and IFITM3 (RC201635) were used to generate expression constructs that enabled the study of IFITM proteins’ roles in cholesterol transport and Aichi virus RNA replication in this paper.
Snippet: A-SAC1, and pCMV-polyprotein was described previously [6,37]. The coding regions of IFITM1, IFITM2, and IFITM3 were amplified by PCR using primer pairs containing MluI–EcoRV sites from cDNA clones (OriGene;IFITM1: RC201617, IFITM2: RC202067, IFITM3: RC201635), respectively, and then inserted into the same sites of pACT and pBIND, resulting in pACT-IFITM1, pACT-IFITM2, pACT-IFITM3, pBIND-IFITM1,
The innate immunity protein IFITM3 modulates γ-secretase in Alzheimer's disease
Hur, Frost, Wu et al
Nature (2020)
Hur, Frost, Wu et al
Nature (2020)
Cited by 400
AI Summary: OriGene’s human IFITM3 plasmid was used to overexpress IFITM3 in cell experiments, enabling the authors to study its effect on γ-secretase activity and amyloid-β production in the context of Alzheimer’s disease.
Snippet: Plasmid DNAs for human IFITM3 (OriGene) were cloned into the pcDNA3.1 vector. U138 EV and U138 Ifitm3−/− cells were transfected with either pcDNA3.1 empty vector or pcDNA3.1 IFITM3 construct using Lipofectamine LTX according to the manufacturer’s instructions and allowed to incubate for 48 h before membrane preparation
25-Hydroxycholesterol Inhibition of Lassa Virus Infection through Aberrant GP1 Glycosylation
Shrivastava-Ranjan, Bergeron, Chakrabarti et al
MBio (2016) 7 (6) DOI: 10.1128/mBio.01808-16
Shrivastava-Ranjan, Bergeron, Chakrabarti et al
MBio (2016) 7 (6) DOI: 10.1128/mBio.01808-16
AI Summary: OriGene contributed to this study by providing the IFITM3 expression plasmid and the anti-Myc antibody, which were used to investigate the molecular mechanisms underlying Lassa virus inhibition.
Snippet: ...IFITM3 were purchased from Origene (Rockville, MD, USA). The following antibodies were used in this study...mixture). The anti-Myc antibody was from Origene, and the mouse monoclonal antiactin antibody was from Sigma-Aldrich...
Showing 1-5 of 5 papers.
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