OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

ZNF416 (NM_017879) Human Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
RC201632
ZNF416 (Myc-DDK-tagged)-Human zinc finger protein 416 (ZNF416). Note: ORF is codon optimized
ORF Plasmid:
DDK tGFP
AAV Particle:
DDK
$289.00 $553.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA339500 Rabbit Polyclonal Anti-ZNF416 Antibody
100 ul
$525.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "ZNF416 or NM_017879"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZNF416
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201632 ORF sequence, codon optimized.
Due to the complexity of NM_017879, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCCGCCGTTCTGCGGGACAGCACTAGTGTTCCCGTCACAGCTGAGGCAAAGCTGATGGGCTTTA
CTCAGGGCTGTGTCACGTTCGAAGACGTAGCCATCTATTTTAGTCAGGAGGAATGGGGACTGCTGGATGA
GGCCCAAAGACTCCTTTATCGAGACGTGATGCTGGAGAACTTCGCACTTATCACAGCCCTGGTATGTTGG
CACGGAATGGAGGACGAAGAGACTCCGGAACAGAGCGTAAGCGTCGAAGGGGTCCCGCAAGTACGAACCC
CCGAAGCTTCCCCCAGCACACAAAAGATCCAGAGTTGCGACATGTGCGTGCCATTTCTCACAGATATACT
GCACCTGACTGATCTGCCCGGCCAGGAGCTTTACTTGACGGGCGCATGTGCCGTGTTCCACCAGGACCAG
AAGCACCACTCCGCTGAAAAGCCCTTGGAGTCCGATATGGACAAGGCTAGCTTTGTTCAATGTTGCCTTT
TTCATGAGTCCGGTATGCCTTTTACAAGTTCTGAGGTCGGAAAGGACTTTCTGGCTCCCCTTGGCATCCT
TCAGCCTCAGGCGATAGCCAATTACGAAAAACCAAATAAGATATCTAAATGTGAGGAAGCTTTTCACGTG
GGGATTAGCCACTACAAATGGTCCCAATGCAGAAGAGAGAGTTCCCATAAACATACTTTCTTTCACCCTA
GAGTTTGCACGGGCAAACGGCTGTACGAATCTAGCAAATGCGGAAAGGCATGCTGCTGCGAGTGTTCCCT
TGTGCAACTGCAGCGAGTGCACCCCGGAGAAAGGCCCTATGAATGCTCAGAGTGCGGCAAGAGCTTTTCT
CAGACATCCCACCTGAACGACCACCGGAGAATCCACACAGGGGAAAGACCGTATGTGTGCGGACAATGCG
GTAAATCATTCTCCCAGCGCGCAACCCTCATCAAGCACCATAGAGTGCACACAGGAGAGCGGCCGTACGA
ATGTGGAGAATGCGGGAAGAGTTTCTCCCAGAGTTCCAACCTGATCGAGCACTGTAGGATCCACACCGGG
GAGCGCCCATACGAGTGCGACGAGTGCGGCAAAGCTTTCGGCTCCAAGAGCACCCTCGTGAGACATCAAA
GAACCCACACGGGTGAGAAGCCCTACGAATGCGGAGAATGCGGCAAACTGTTTCGGCAGTCTTTCAGCCT
GGTGGTGCACCAGAGGATCCACACAACCGCTCGGCCTTACGAATGTGGGCAATGCGGTAAGTCTTTCTCT
CTGAAATGCGGGTTGATCCAGCACCAGCTTATTCACTCCGGCGCCCGACCATTTGAGTGCGATGAGTGCG
GTAAGTCTTTTTCTCAACGCACTACACTCAACAAACATCACAAGGTTCACACAGCGGAGCGACCCTATGT
GTGCGGAGAGTGCGGCAAAGCTTTCATGTTCAAGTCCAAACTGGTCAGACATCAACGGACTCATACAGGC
GAACGCCCCTTCGAGTGCAGCGAATGCGGCAAGTTTTTCCGCCAAAGTTACACCTTGGTGGAGCATCAGA
AAATTCACACTGGTCTGCGACCCTACGACTGCGGTCAGTGCGGAAAGTCTTTCATTCAGAAGAGTTCACT
CATCCAGCATCAAGTGGTTCACACGGGAGAACGCCCTTATGAATGTGGAAAGTGCGGCAAGAGCTTCACG
CAGCATTCCGGACTTATCCTCCATCGGAAGAGTCACACAGTGGAAAGGCCCCGAGATTCAAGCAAATGTG
GGAAGCCCTACAGTCCCAGATCTAATATTGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201632 representing NM_017879
Red=Cloning site Green=Tags(s)

MAAAVLRDSTSVPVTAEAKLMGFTQGCVTFEDVAIYFSQEEWGLLDEAQRLLYRDVMLENFALITALVCW
HGMEDEETPEQSVSVEGVPQVRTPEASPSTQKIQSCDMCVPFLTDILHLTDLPGQELYLTGACAVFHQDQ
KHHSAEKPLESDMDKASFVQCCLFHESGMPFTSSEVGKDFLAPLGILQPQAIANYEKPNKISKCEEAFHV
GISHYKWSQCRRESSHKHTFFHPRVCTGKRLYESSKCGKACCCECSLVQLQRVHPGERPYECSECGKSFS
QTSHLNDHRRIHTGERPYVCGQCGKSFSQRATLIKHHRVHTGERPYECGECGKSFSQSSNLIEHCRIHTG
ERPYECDECGKAFGSKSTLVRHQRTHTGEKPYECGECGKLFRQSFSLVVHQRIHTTARPYECGQCGKSFS
LKCGLIQHQLIHSGARPFECDECGKSFSQRTTLNKHHKVHTAERPYVCGECGKAFMFKSKLVRHQRTHTG
ERPFECSECGKFFRQSYTLVEHQKIHTGLRPYDCGQCGKSFIQKSSLIQHQVVHTGERPYECGKCGKSFT
QHSGLILHRKSHTVERPRDSSKCGKPYSPRSNIV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017879
ORF Size 1782 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017879.1, NP_060349.1
RefSeq Size 2545 bp
RefSeq ORF 1785 bp
Locus ID 55659
UniProt ID Q9BWM5
Cytogenetics 19q13.43
MW 67.2 kDa
Summary May be involved in transcriptional regulation.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_017879" in other vectors (2)
SKU Description Size Price
RG201632 ZNF416 (tGFP-tagged) - Human zinc finger protein 416 (ZNF416). Note: ORF is codon optimized 10 ug
$489.00 $753.00
SC324576 ZNF416 (untagged)-Human zinc finger protein 416 (ZNF416) 10 ug
$554.00

Plasmid Map for RC201632

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201632
Circular map for RC201632

file-alt Citations

ZNF416 is a pivotal transcriptional regulator of fibroblast mechanoactivation
Jones, Meridew, Link et al
J Cell Biol (2021) 220 (5) DOI: 10.1083/jcb.202007152
Cited by 47
AI Summary: OriGene products, specifically the ZNF416 cDNA clone (Cat# RC223491) and ZNF416 shRNA (Cat# TL313366), were used in this study to manipulate ZNF416 expression in fibroblasts, enabling the authors to investigate its pivotal role as a transcriptional regulator in fibroblast mechanoactivation.
FigureFigureFigure
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products