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Annexin A1 (ANXA1) (NM_000700) Human Tagged ORF Clone

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SKU
RC201569
ANXA1 (Myc-DDK-tagged)-Human annexin A1 (ANXA1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Annexin A1 or NM_000700"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Annexin A1
Synonyms ANX1; LPC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201569 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAATGGTATCAGAATTCCTCAAGCAGGCCTGGTTTATTGAAAATGAAGAGCAGGAATATGTTCAAA
CTGTGAAGTCATCCAAAGGTGGTCCCGGATCAGCGGTGAGCCCCTATCCTACCTTCAATCCATCCTCGGA
TGTCGCTGCCTTGCATAAGGCCATAATGGTTAAAGGTGTGGATGAAGCAACCATCATTGACATTCTAACT
AAGCGAAACAATGCACAGCGTCAACAGATCAAAGCAGCATATCTCCAGGAAACAGGAAAGCCCCTGGATG
AAACACTGAAGAAAGCCCTTACAGGTCACCTTGAGGAGGTTGTTTTAGCTCTGCTAAAAACTCCAGCGCA
ATTTGATGCTGATGAACTTCGTGCTGCCATGAAGGGCCTTGGAACTGATGAAGATACTCTAATTGAGATT
TTGGCATCAAGAACTAACAAAGAAATCAGAGACATTAACAGGGTCTACAGAGAGGAACTGAAGAGAGATC
TGGCCAAAGACATAACCTCAGACACATCTGGAGATTTTCGGAACGCTTTGCTTTCTCTTGCTAAGGGTGA
CCGATCTGAGGACTTTGGTGTGAATGAAGACTTGGCTGATTCAGATGCCAGGGCCTTGTATGAAGCAGGA
GAAAGGAGAAAGGGGACAGACGTAAACGTGTTCAATACCATCCTTACCACCAGAAGCTATCCACAACTTC
GCAGAGTGTTTCAGAAATACACCAAGTACAGTAAGCATGACATGAACAAAGTTCTGGACCTGGAGTTGAA
AGGTGACATTGAGAAATGCCTCACAGCTATCGTGAAGTGCGCCACAAGCAAACCAGCTTTCTTTGCAGAG
AAGCTTCATCAAGCCATGAAAGGTGTTGGAACTCGCCATAAGGCATTGATCAGGATTATGGTTTCCCGTT
CTGAAATTGACATGAATGATATCAAAGCATTCTATCAGAAGATGTATGGTATCTCCCTTTGCCAAGCCAT
CCTGGATGAAACCAAAGGAGATTATGAGAAAATCCTGGTGGCTCTTTGTGGAGGAAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201569 protein sequence
Red=Cloning site Green=Tags(s)

MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILT
KRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEI
LASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAG
ERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAE
KLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000700
ORF Size 1038 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000700.3
RefSeq Size 1399 bp
RefSeq ORF 1041 bp
Locus ID 301
UniProt ID P04083
Cytogenetics 9q21.13
Domains annexin
Protein Families Druggable Genome
MW 38.7 kDa
Summary This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. provided by RefSeq, Dec 2014
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Resources
"NM_000700" in other vectors (6)
SKU Description Size Price
RC201569L1 Lenti ORF clone of Human annexin A1 (ANXA1), Myc-DDK-tagged 10 ug
$757.00
RC201569L2 Lenti ORF clone of Human annexin A1 (ANXA1), mGFP tagged 10 ug
$757.00
RC201569L3 Lenti ORF clone of Human annexin A1 (ANXA1), Myc-DDK-tagged 10 ug
$757.00
RC201569L4 Lenti ORF clone of Human annexin A1 (ANXA1), mGFP tagged 10 ug
$757.00
RG201569 ANXA1 (tGFP-tagged) - Human annexin A1 (ANXA1) 10 ug
$489.00 $657.00
SC119713 ANXA1 (untagged)-Human annexin A1 (ANXA1) 10 ug
$686.00

Plasmid Map for RC201569

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201569
Circular map for RC201569

file-alt Citations

The Arg/N-degron pathway mediates the secretion of apoptotic exosomes under oxidative stress in cancer cell
Kim, Lee, Jung et al
iScience (2025) 28 (6), 112637 DOI: 10.1016/j.isci.2025.112637
Cited by 1
AI Summary: In this study, OriGene products RILP-MYC,FLAG (Cat#RC206400), VPS22-MYC,FLAG (Cat#RC202137), VPS36-GFP (Cat#RG237476), and ANXA1-MYC,FLAG (Cat#RC201569) were used as tagged protein constructs to investigate the molecular mechanisms underlying apoptotic exosome secretion in cancer cells under oxidative stress.
Snippet: RILP-MYC,FLAG Origene Cat#RC206400 Cleaved-RILP-MYC,FLAG This paper VPS22-MYC,FLAG Origene Cat#RC202137 VPS36-GFP Origene Cat#RG237476 Caspase1-MYC Addgene Cat#41552 ARL8B-V5 Addgene Cat#67446 KLC-MCHERRY Addgene Cat#62747 ANXA1-MYC,FLAG Origene Cat#RC201569
Showing 1-1 of 1 papers.
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