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TMEM33 (NM_018126) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC201488
TMEM33 (Myc-DDK-tagged)-Human transmembrane protein 33 (TMEM33)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMEM33
Synonyms 1600019D15Rik; Pom33; SHINC-3; SHINC3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201488 representing NM_018126
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGATACGACCCCGAACGGCCCCCAAGGGGCGGGCGCTGTGCAATTCATGATGACCAATAAACTGG
ACACGGCAATGTGGCTTTCTCGCTTGTTCACAGTTTACTGCTCTGCTCTGTTTGTTCTGCCTCTTCTTGG
GTTGCATGAAGCAGCAAGCTTTTACCAACGTGCTTTGCTGGCAAATGCTCTTACCAGTGCTCTGAGGCTG
CATCAAAGATTACCACACTTCCAGTTAAGCAGAGCATTCCTGGCCCAGGCTTTGTTAGAGGACAGCTGCC
ACTACCTGTTGTATTCACTCATCTTTGTAAATTCCTATCCAGTTACAATGAGTATCTTCCCAGTCTTGTT
ATTCTCTTTGCTTCATGCTGCCACATATACGAAAAAGGTCCTTGACGCAAGGGGCTCAAATAGTTTACCT
CTGCTGAGATCTGTCTTGGACAAATTAAGTGCTAATCAACAAAATATTCTGAAATTCATTGCTTGCAATG
AAATATTCCTGATGCCTGCGACAGTTTTTATGCTTTTTAGTGGTCAAGGAAGTTTGCTCCAACCTTTTAT
ATACTATAGATTTCTTACCCTTCGATATTCGTCTCGAAGAAACCCATATTGTCGGACCTTATTTAATGAA
CTGAGGATTGTTGTTGAACACATAATAATGAAACCTGCTTGCCCACTGTTTGTGAGAAGACTTTGTCTCC
AGAGCATTGCCTTTATAAGCAGATTGGCACCAACAGTTCCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201488 representing NM_018126
Red=Cloning site Green=Tags(s)

MADTTPNGPQGAGAVQFMMTNKLDTAMWLSRLFTVYCSALFVLPLLGLHEAASFYQRALLANALTSALRL
HQRLPHFQLSRAFLAQALLEDSCHYLLYSLIFVNSYPVTMSIFPVLLFSLLHAATYTKKVLDARGSNSLP
LLRSVLDKLSANQQNILKFIACNEIFLMPATVFMLFSGQGSLLQPFIYYRFLTLRYSSRRNPYCRTLFNE
LRIVVEHIIMKPACPLFVRRLCLQSIAFISRLAPTVP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018126
ORF Size 741 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018126.3
RefSeq Size 2684 bp
RefSeq ORF 744 bp
Locus ID 55161
UniProt ID P57088
Cytogenetics 4p13
Domains UPF0121
Protein Families Transmembrane
MW 27.8 kDa
Summary Acts as a regulator of the tubular endoplasmic reticulum (ER) network. Suppresses the RTN3/4-induced formation of the ER tubules (PubMed:25612671). Positively regulates PERK-mediated and IRE1-mediated unfolded protein response signaling (PubMed:26268696).UniProtKB/Swiss-Prot Function
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Documents
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"NM_018126" in other vectors (7)
SKU Description Size Price
RC201488L1 Lenti-ORF clone of TMEM33 (Myc-DDK-tagged)-Human transmembrane protein 33 (TMEM33) 10 ug
$600.00
RC201488L2 Lenti-ORF clone of TMEM33 (mGFP-tagged)-Human transmembrane protein 33 (TMEM33) 10 ug
$600.00
RC201488L3 Lenti-ORF clone of TMEM33 (Myc-DDK-tagged)-Human transmembrane protein 33 (TMEM33) 10 ug
$600.00
RC201488L4 Lenti-ORF clone of TMEM33 (mGFP-tagged)-Human transmembrane protein 33 (TMEM33) 10 ug
$600.00
RG201488 TMEM33 (tGFP-tagged) - Human transmembrane protein 33 (TMEM33) 10 ug
$489.00 $500.00
SC113689 TMEM33 (untagged)-Human transmembrane protein 33 (TMEM33) 10 ug
$300.00
SC322160 TMEM33 (untagged)-Human transmembrane protein 33 (TMEM33) 10 ug
$300.00

Plasmid Map for RC201488

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201488
Circular map for RC201488

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