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HENMT1 (NM_144584) Human Tagged ORF Clone

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SKU
RC201373
HENMT1 (Myc-DDK-tagged)-Human HEN1 methyltransferase homolog 1 (Arabidopsis) (HENMT1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HENMT1
Synonyms C1orf59; HEN1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201373 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGAAAATAATCTACAGTGCAGTAGTGTGGTTGACGGTAATTTTGAAGAAGTTCCCAGGGAGACGG
CAATTCAGTTTAAACCTCCACTATACAGACAGCGGTACCAGTTCGTTAAAAATTTAGTGGATCAACATGA
GCCTAAGAAGGTTGCAGACCTGGGATGTGGTGATACTTCACTCTTAAGGCTGCTAAAAGTCAATCCATGC
ATTGAATTGCTTGTTGGAGTAGATATTAATGAGGATAAATTACGATGGAGAGGGGATTCGTTAGCTCCTT
TCCTGGGGGATTTTCTGAAACCTCGGGATCTGAATTTGACCATCACATTGTATCATGGCTCCGTTGTGGA
GAGAGACTCTCGTTTGCTTGGATTTGACTTGATAACGTGTATTGAATTAATAGAACATTTGGATTCAGGT
GATCTGGCCAGATTTCCTGAAGTGGTATTTGGGTACCTGTCTCCATCCATGATTGTCATCAGCACACCAA
ACTCTGAATTCAATCCCCTGTTTCCATCAGTGACCTTAAGAGATTCAGATCATAAATTTGAGTGGACCAG
AATGGAGTTTCAGACCTGGGCTTTATATGTGGCAAATCGCTATGATTACTCTGTGGAGTTTACTGGTGTC
GGGGAACCACCAGCTGGAGCTGAGAATGTTGGATACTGTACCCAGATAGGAATCTTCCGGAAAAATGGAG
GAAAGGCAACAGAATCATGTCTTTCAGAGCAGCATGATCAGCATGTTTATAAAGCTGTTTTTACCACCTC
ATACCCAAGCTTACAGCAGGAAAGGTTCTTTAAACTTGTGTTGGTTAATGAGGTGTCCCAACAAGTGGAA
AGCTTAAGAGTGAGCCACCTGCCAAGGCGGAAAGAACAGGCTGGGGAACGGGGTGATAAGCCCAAAGACA
TTGGTGGCTCAAAGGCCCCTGTCCCATGCTTTGGACCAGTCTTCACAGAGGTTGAGAAGGCCAAGATAGA
GAACTCTCCCACACCCTTCTGTGTTGGAGATAAATTTTTCGTACCTCTGCAGAGACTCCTTGCGTATCCC
AAGTTGAACCGCTTATGTGCTAATGAAGAGATTATGAGATCAGTCATTGCTGACTCAATTCCTCTGAGCA
GTGATGGTTCTGCAGTGGTGGCTGACCTGCGTAATTATTTTGATGAACAGTTTGAGTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201373 protein sequence
Red=Cloning site Green=Tags(s)

MEENNLQCSSVVDGNFEEVPRETAIQFKPPLYRQRYQFVKNLVDQHEPKKVADLGCGDTSLLRLLKVNPC
IELLVGVDINEDKLRWRGDSLAPFLGDFLKPRDLNLTITLYHGSVVERDSRLLGFDLITCIELIEHLDSG
DLARFPEVVFGYLSPSMIVISTPNSEFNPLFPSVTLRDSDHKFEWTRMEFQTWALYVANRYDYSVEFTGV
GEPPAGAENVGYCTQIGIFRKNGGKATESCLSEQHDQHVYKAVFTTSYPSLQQERFFKLVLVNEVSQQVE
SLRVSHLPRRKEQAGERGDKPKDIGGSKAPVPCFGPVFTEVEKAKIENSPTPFCVGDKFFVPLQRLLAYP
KLNRLCANEEIMRSVIADSIPLSSDGSAVVADLRNYFDEQFEF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144584
ORF Size 1179 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144584.2
RefSeq Size 1890 bp
RefSeq ORF 1182 bp
Locus ID 113802
UniProt ID Q5T8I9
Cytogenetics 1p13.3
MW 44.5 kDa
Summary Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis.UniProtKB/Swiss-Prot Function
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"NM_144584" in other vectors (6)
SKU Description Size Price
RC201373L1 Lenti ORF clone of Human HEN1 methyltransferase homolog 1 (Arabidopsis) (HENMT1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC201373L2 Lenti ORF clone of Human HEN1 methyltransferase homolog 1 (Arabidopsis) (HENMT1), transcript variant 1, mGFP tagged 10 ug
$757.00
RC201373L3 Lenti ORF clone of Human HEN1 methyltransferase homolog 1 (Arabidopsis) (HENMT1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC201373L4 Lenti ORF clone of Human HEN1 methyltransferase homolog 1 (Arabidopsis) (HENMT1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG201373 HENMT1 (tGFP-tagged) - Human HEN1 methyltransferase homolog 1 (Arabidopsis) (HENMT1), transcript variant 1 10 ug
$489.00 $657.00
SC120736 HENMT1 (untagged)-Human HEN1 methyltransferase homolog 1 (Arabidopsis) (HENMT1), transcript variant 1 10 ug
$457.00

Plasmid Map for RC201373

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201373
Circular map for RC201373

file-alt Citations

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