FADS3 (NM_021727) Human Tagged ORF Clone

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SKU
RC201302
FADS3 (Myc-DDK-tagged)-Human fatty acid desaturase 3 (FADS3)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FADS3
Synonyms CYB5RP; LLCDL3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201302 representing NM_021727
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGGCGTCGGGGAGCCGGGACCGCGGGAGGGACCCGCGCAGCCGGGGGCGCCGCTGCCCACCTTCT
GCTGGGAGCAGATCCGCGCGCACGACCAGCCCGGCGACAAGTGGCTGGTCATCGAGCGCCGCGTCTACGA
CATCAGCCGCTGGGCACAGCGGCACCCAGGGGGCAGCCGCCTCATCGGCCACCACGGCGCTGAGGACGCC
ACGGATGCCTTCCGTGCCTTCCATCAAGATCTCAATTTTGTGCGCAAGTTCCTACAGCCCCTGTTGATTG
GAGAGCTGGCTCCGGAAGAACCCAGCCAGGATGGACCCCTGAATGCGCAGCTGGTCGAGGACTTCCGAGC
CCTGCACCAGGCAGCCGAGGACATGAAGCTGTTTGATGCCAGTCCCACCTTCTTTGCTTTCCTACTGGGC
CACATCCTGGCCATGGAGGTGCTGGCCTGGCTCCTTATCTACCTCCTGGGTCCTGGCTGGGTGCCCAGTG
CCCTGGCCGCCTTCATCCTGGCCATCTCTCAGGCTCAGTCCTGGTGTCTGCAGCATGACCTGGGCCATGC
CTCCATCTTCAAGAAGTCCTGGTGGAACCACGTGGCCCAGAAGTTCGTGATGGGGCAGCTAAAGGGCTTC
TCCGCCCACTGGTGGAACTTCCGCCACTTCCAGCACCACGCCAAGCCCAACATCTTCCACAAAGACCCAG
ACGTGACGGTGGCGCCCGTCTTCCTCCTGGGGGAGTCATCCGTCGAGTATGGCAAGAAGAAACGCAGATA
CCTACCCTACAACCAGCAGCACCTGTACTTCTTCCTGATCGGCCCGCCGCTGCTCACCCTGGTGAACTTT
GAAGTGGAAAATCTGGCGTACATGCTGGTGTGCATGCAGTGGGCGGATTTGCTCTGGGCCGCCAGCTTCT
ATGCCCGCTTCTTCTTATCCTACCTCCCCTTCTACGGCGTCCCTGGGGTGCTGCTCTTCTTTGTTGCTGT
CAGGGTCCTGGAAAGCCACTGGTTCGTGTGGATCACACAGATGAACCACATCCCCAAGGAGATCGGCCAC
GAGAAGCACCGGGACTGGGTCAGCTCTCAGCTGGCAGCCACCTGCAACGTGGAGCCCTCACTTTTCACCA
ACTGGTTCAGCGGGCACCTCAACTTCCAGATCGAGCACCACCTCTTCCCCAGGATGCCGAGACACAACTA
CAGCCGGGTGGCCCCGCTGGTCAAGTCGCTGTGTGCCAAGCACGGCCTCAGCTACGAAGTGAAGCCCTTC
CTCACCGCGCTGGTGGACATCGTCAGGTCCCTGAAGAAGTCTGGTGACATCTGGCTGGACGCCTACCTCC
ATCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201302 representing NM_021727
Red=Cloning site Green=Tags(s)

MGGVGEPGPREGPAQPGAPLPTFCWEQIRAHDQPGDKWLVIERRVYDISRWAQRHPGGSRLIGHHGAEDA
TDAFRAFHQDLNFVRKFLQPLLIGELAPEEPSQDGPLNAQLVEDFRALHQAAEDMKLFDASPTFFAFLLG
HILAMEVLAWLLIYLLGPGWVPSALAAFILAISQAQSWCLQHDLGHASIFKKSWWNHVAQKFVMGQLKGF
SAHWWNFRHFQHHAKPNIFHKDPDVTVAPVFLLGESSVEYGKKKRRYLPYNQQHLYFFLIGPPLLTLVNF
EVENLAYMLVCMQWADLLWAASFYARFFLSYLPFYGVPGVLLFFVAVRVLESHWFVWITQMNHIPKEIGH
EKHRDWVSSQLAATCNVEPSLFTNWFSGHLNFQIEHHLFPRMPRHNYSRVAPLVKSLCAKHGLSYEVKPF
LTALVDIVRSLKKSGDIWLDAYLHQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021727
ORF Size 1335 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021727.5
RefSeq Size 1798 bp
RefSeq ORF 1338 bp
Locus ID 3995
UniProt ID Q9Y5Q0, A0A024R564
Cytogenetics 11q12.2
Domains FA_desaturase, heme_1
Protein Families Transmembrane
MW 51.6 kDa
Summary The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_021727" in other vectors (4)
SKU Description Size Price
RC201302L3 Lenti-ORF clone of FADS3 (Myc-DDK-tagged)-Human fatty acid desaturase 3 (FADS3) 10 ug
$986.00
RC201302L4 Lenti-ORF clone of FADS3 (mGFP-tagged)-Human fatty acid desaturase 3 (FADS3) 10 ug
$986.00
RG201302 FADS3 (tGFP-tagged) - Human fatty acid desaturase 3 (FADS3) 10 ug
$886.00
SC319457 FADS3 (untagged)-Human fatty acid desaturase 3 (FADS3) 10 ug
$686.00

Plasmid Map for RC201302

Download sequence for

Cloning scheme for RC201302

Circular map for RC201302

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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