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SUPT5H (NM_003169) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC201196
SUPT5H (Myc-DDK-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$589.00 $946.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "SUPT5H or NM_003169"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SUPT5H
Synonyms SPT5; SPT5H; Tat-CT1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201196 representing NM_003169
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGACAGCGAGGACAGCAACTTTTCCGAGGAGGAGGACAGCGAGCGCAGCAGTGACGGCGAGGAGG
CCGAGGTAGACGAAGAGCGGCGGAGTGCAGCGGGCAGTGAGAAAGAAGAAGAGCCTGAGGACGAAGAGGA
GGAGGAAGAGGAGGAGGAATATGATGAGGAAGAGGAAGAAGAAGATGATGACCGACCCCCCAAGAAACCC
CGCCATGGAGGCTTCATTCTGGACGAGGCTGATGTTGACGATGAGTATGAGGACGAGGACCAGTGGGAGG
ATGGAGCAGAGGACATTCTAGAGAAAGAAGAGATTGAAGCCTCCAATATCGATAATGTTGTCCTGGATGA
AGATCGTTCTGGGGCTCGCCGCCTGCAAAACCTCTGGAGGGACCAGCGAGAAGAAGAACTGGGCGAGTAT
TACATGAAGAAATACGCCAAGTCATCTGTGGGAGAGACGGTGTATGGAGGATCTGATGAGCTCTCAGACG
ACATCACCCAGCAGCAGCTGCTCCCAGGAGTCAAGGATCCCAATCTGTGGACTGTCAAATGTAAGATTGG
GGAGGAACGGGCCACGGCCATTTCCTTGATGCGCAAGTTCATTGCCTACCAGTTCACAGACACGCCCCTG
CAGATCAAGTCAGTAGTGGCACCAGAGCATGTGAAGGGCTACATCTACGTGGAGGCCTACAAGCAGACCC
ACGTGAAGCAGGCCATTGAGGGGGTGGGCAACCTGCGGCTTGGCTACTGGAACCAGCAGATGGTGCCCAT
CAAGGAGATGACAGACGTGCTCAAAGTGGTGAAGGAGGTGGCCAACCTGAAACCAAAGTCCTGGGTCCGC
CTCAAGCGGGGCATCTACAAGGATGACATTGCTCAGGTGGACTACGTGGAGCCCAGCCAGAACACCATCT
CCCTGAAGATGATCCCACGCATCGACTACGATCGCATCAAGGCCCGCATGAGCTTGAAAGACTGGTTTGC
CAAAAGGAAGAAGTTTAAGCGGCCTCCACAGAGGCTGTTTGATGCTGAGAAGATCAGGTCCCTGGGGGGT
GATGTTGCCTCTGATGGTGACTTCCTCATCTTTGAGGGGAACCGTTACAGCCGGAAGGGCTTTCTGTTCA
AGAGCTTCGCCATGTCTGCTGTGATCACGGAGGGTGTGAAGCCAACACTCTCTGAGCTGGAAAAGTTTGA
GGACCAGCCAGAGGGCATTGACCTGGAGGTGGTGACTGAGAGCACAGGGAAGGAGCGGGAGCACAACTTC
CAACCTGGGGACAACGTGGAGGTCTGTGAGGGTGAGCTCATCAACCTGCAGGGCAAGATCCTCAGCGTGG
ATGGCAACAAGATCACCATCATGCCCAAGCATGAGGACCTCAAGGACATGTTGGAGTTCCCAGCCCAGGA
ACTTAGAAAATACTTCAAGATGGGGGACCACGTGAAGGTGATTGCTGGCCGATTCGAGGGCGACACAGGC
CTCATTGTGCGGGTGGAGGAGAATTTCGTTATCCTGTTCTCTGACCTCACCATGCATGAGCTGAAGGTGC
TCCCCCGGGACCTGCAGCTCTGCTCAGAGACAGCATCAGGTGTGGATGTTGGGGGCCAGCATGAATGGGG
CGAGCTGGTGCAGCTGGATCCCCAGACTGTGGGTGTCATCGTGCGACTAGAACGGGAGACCTTCCAGGTG
CTGAACATGTACGGGAAGGTGGTGACTGTCAGACATCAGGCTGTGACCCGGAAGAAGGACAACCGCTTTG
CTGTGGCCTTGGACTCAGAGCAGAACAACATCCATGTGAAAGACATCGTTAAGGTCATTGATGGCCCCCA
CTCAGGCCGAGAAGGGGAGATTCGCCATCTCTTCCGAAGCTTCGCCTTCCTACATTGCAAGAAACTGGTG
GAGAACGGGGGCATGTTTGTCTGCAAGACCCGCCACCTGGTGCTGGCTGGGGGCTCAAAGCCCCGTGATG
TGACCAACTTCACCGTGGGTGGCTTTGCGCCTATGAGTCCCCGGATCAGCAGCCCCATGCACCCCAGTGC
TGGAGGTCAGCGTGGCGGCTTTGGTAGCCCAGGTGGCGGCAGTGGTGGCATGAGCAGGGGCCGGGGCCGG
AGGGACAACGAACTCATCGGCCAGACCGTGCGCATCTCCCAGGGGCCCTACAAAGGCTACATCGGTGTGG
TGAAAGATGCCACAGAGTCCACGGCCCGTGTGGAGCTGCACTCCACCTGCCAGACCATCTCTGTGGACCG
TCAGCGGCTCACCACGGTGGGCTCACGGCGCCCGGGCGGCATGACCTCGACCTATGGGAGGACGCCCATG
TATGGCTCCCAGACGCCCATGTATGGCTCTGGCTCCCGAACACCCATGTACGGCTCACAGACACCCCTCC
AGGATGGTAGCCGCACCCCACACTACGGCTCACAGACGCCCCTGCATGATGGCAGCCGCACTCCTGCCCA
GAGTGGGGCCTGGGACCCCAACAACCCCAACACGCCGTCACGGGCTGAGGAAGAATATGAGTATGCTTTC
GATGATGAGCCCACCCCGTCCCCGCAGGCCTATGGGGGAACCCCCAATCCCCAAACACCTGGCTACCCAG
ACCCCTCGTCCCCACAGGTCAACCCACAATACAACCCGCAGACGCCAGGGACGCCGGCCATGTACAACAC
AGACCAGTTCTCTCCCTATGCTGCCCCCTCCCCACAAGGTTCCTACCAGCCCAGCCCCAGCCCCCAGAGC
TACCACCAGGTGGCGCCAAGCCCAGCAGGCTACCAGAATACCCACTCCCCAGCCAGCTACCACCCTACAC
CGTCGCCCATGGCCTATCAGGCTAGCCCCAGCCCGAGCCCCGTTGGCTACAGTCCTATGACACCTGGAGC
TCCCTCCCCTGGTGGCTACAACCCACACACGCCAGGCTCAGGCATCGAGCAGAACTCCAGCGACTGGGTA
ACCACTGACATTCAGGTGAAGGTGCGGGACACCTACCTGGATACACAGGTGGTGGGACAGACAGGTGTCA
TCCGCAGTGTCACGGGGGGCATGTGCTCTGTGTACCTGAAGGACAGTGAGAAGGTTGTCAGCATTTCCAG
TGAGCACCTGGAGCCTATCACCCCCACCAAGAACAACAAGGTGAAAGTGATCCTGGGCGAGGATCGGGAA
GCCACGGGCGTCCTACTGAGCATTGATGGTGAGGATGGCATTGTCCGTATGGACCTTGATGAGCAGCTCA
AGATCCTCAACCTCCGCTTCCTGGGGAAGCTCCTGGAAGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201196 representing NM_003169
Red=Cloning site Green=Tags(s)

MSDSEDSNFSEEEDSERSSDGEEAEVDEERRSAAGSEKEEEPEDEEEEEEEEEYDEEEEEEDDDRPPKKP
RHGGFILDEADVDDEYEDEDQWEDGAEDILEKEEIEASNIDNVVLDEDRSGARRLQNLWRDQREEELGEY
YMKKYAKSSVGETVYGGSDELSDDITQQQLLPGVKDPNLWTVKCKIGEERATAISLMRKFIAYQFTDTPL
QIKSVVAPEHVKGYIYVEAYKQTHVKQAIEGVGNLRLGYWNQQMVPIKEMTDVLKVVKEVANLKPKSWVR
LKRGIYKDDIAQVDYVEPSQNTISLKMIPRIDYDRIKARMSLKDWFAKRKKFKRPPQRLFDAEKIRSLGG
DVASDGDFLIFEGNRYSRKGFLFKSFAMSAVITEGVKPTLSELEKFEDQPEGIDLEVVTESTGKEREHNF
QPGDNVEVCEGELINLQGKILSVDGNKITIMPKHEDLKDMLEFPAQELRKYFKMGDHVKVIAGRFEGDTG
LIVRVEENFVILFSDLTMHELKVLPRDLQLCSETASGVDVGGQHEWGELVQLDPQTVGVIVRLERETFQV
LNMYGKVVTVRHQAVTRKKDNRFAVALDSEQNNIHVKDIVKVIDGPHSGREGEIRHLFRSFAFLHCKKLV
ENGGMFVCKTRHLVLAGGSKPRDVTNFTVGGFAPMSPRISSPMHPSAGGQRGGFGSPGGGSGGMSRGRGR
RDNELIGQTVRISQGPYKGYIGVVKDATESTARVELHSTCQTISVDRQRLTTVGSRRPGGMTSTYGRTPM
YGSQTPMYGSGSRTPMYGSQTPLQDGSRTPHYGSQTPLHDGSRTPAQSGAWDPNNPNTPSRAEEEYEYAF
DDEPTPSPQAYGGTPNPQTPGYPDPSSPQVNPQYNPQTPGTPAMYNTDQFSPYAAPSPQGSYQPSPSPQS
YHQVAPSPAGYQNTHSPASYHPTPSPMAYQASPSPSPVGYSPMTPGAPSPGGYNPHTPGSGIEQNSSDWV
TTDIQVKVRDTYLDTQVVGQTGVIRSVTGGMCSVYLKDSEKVVSISSEHLEPITPTKNNKVKVILGEDRE
ATGVLLSIDGEDGIVRMDLDEQLKILNLRFLGKLLEA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003169
ORF Size 3261 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003169.3
RefSeq Size 3762 bp
RefSeq ORF 3264 bp
Locus ID 6829
UniProt ID O00267
Cytogenetics 19q13.2
Domains KOW, NGN, Supt5
Protein Families Transcription Factors
MW 120.8 kDa
Summary Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.UniProtKB/Swiss-Prot Function
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"NM_003169" in other vectors (6)
SKU Description Size Price
RC201196L1 Lenti-ORF clone of SUPT5H (Myc-DDK-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1 10 ug
$1,246.00
RC201196L2 Lenti-ORF clone of SUPT5H (mGFP-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1 10 ug
$1,246.00
RC201196L3 Lenti-ORF clone of SUPT5H (Myc-DDK-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1 10 ug
$1,246.00
RC201196L4 Lenti-ORF clone of SUPT5H (mGFP-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1 10 ug
$1,246.00
RG201196 SUPT5H (tGFP-tagged) - Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1 10 ug
$1,146.00
SC118151 SUPT5H (untagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1 10 ug
$947.00

Plasmid Map for RC201196

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201196
Circular map for RC201196

file-alt Citations

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