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PAXX (NM_183241) Human Tagged ORF Clone

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SKU
RC200944
C9orf142 (Myc-DDK-tagged)-Human chromosome 9 open reading frame 142 (C9orf142)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PAXX
Synonyms C9orf142; XLS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200944 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCCGCTGTCGCCGCCGCTCTGCACGCTGCCGCCGGGCCCCGAGCCGCCCCGCTTCGTGTGCTACT
GCGAAGGGGAGGAAAGCGGGGAGGGGGACCGCGGCGGCTTCAACCTCTACGTGACCGACGCCGCGGAGCT
TTGGAGCACCTGCTTCACGCCGGACAGCCTGGCGGCCCTCAAAGCCCGTTTTGGCCTGAGTGCGGCTGAG
GACATCACCCCCCGGTTCAGGGCAGCCTGTGAGCAGCAAGCTGTGGCTCTGACTCTGCAGGAGGACAGAG
CATCCCTGACGCTTTCAGGGGGGCCCTCGGCACTGGCCTTTGACCTCTCCAAGGTACCAGGCCCAGAGGC
AGCCCCCAGGCTGCGGGCGCTGACACTGGGCCTGGCAAAACGCGTGTGGAGCCTGGAGCGGCGACTGGCA
GCTGCAGAAGAGACAGCTGTCAGCCCGAGGAAGAGCCCCCGGCCTGCAGGGCCTCAGCTCTTCTTACCAG
ACCCAGATCCCCAGAGAGGTGGCCCTGGACCTGGAGTCAGGAGGCGGTGTCCAGGAGAGTCGCTCATCAA
CCCCGGGTTCAAGAGTAAGAAACCAGCTGGTGGCGTGGACTTCGATGAGACC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200944 protein sequence
Red=Cloning site Green=Tags(s)

MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAALKARFGLSAAE
DITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPRLRALTLGLAKRVWSLERRLA
AAEETAVSPRKSPRPAGPQLFLPDPDPQRGGPGPGVRRRCPGESLINPGFKSKKPAGGVDFDET

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_183241
ORF Size 612 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183241.3
RefSeq Size 851 bp
RefSeq ORF 615 bp
Locus ID 286257
UniProt ID Q9BUH6
Cytogenetics 9q34.3
MW 21.6 kDa
Summary The protein encoded by this gene plays a role in the nonhomologous end joining (NHEJ) pathway of DNA double-strand break repair. The encoded protein may function to stabilize the Ku70/Ku80 heterodimer to facilitate the assembly and maintain the stability of the NHEJ complex. provided by RefSeq, Jul 2016
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"NM_183241" in other vectors (6)
SKU Description Size Price
RC200944L1 Lenti ORF clone of Human chromosome 9 open reading frame 142 (C9orf142), Myc-DDK-tagged 10 ug
$600.00
RC200944L2 Lenti ORF clone of Human chromosome 9 open reading frame 142 (C9orf142), mGFP tagged 10 ug
$600.00
RC200944L3 Lenti ORF clone of Human chromosome 9 open reading frame 142 (C9orf142), Myc-DDK-tagged 10 ug
$600.00
RC200944L4 Lenti ORF clone of Human chromosome 9 open reading frame 142 (C9orf142), mGFP tagged 10 ug
$600.00
RG200944 C9orf142 (tGFP-tagged) - Human chromosome 9 open reading frame 142 (C9orf142) 10 ug
$489.00 $500.00
SC319880 C9orf142 (untagged)-Human chromosome 9 open reading frame 142 (C9orf142) 10 ug
$300.00

Plasmid Map for RC200944

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200944
Circular map for RC200944

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