CCDC124 (NM_138442) Human Tagged ORF Clone

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SKU
RC200867
CCDC124 (Myc-DDK-tagged)-Human coiled-coil domain containing 124 (CCDC124), transcript variant 1
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCDC124
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200867 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAAGAAGTTCCAGGGTGAGAACACCAAGTCGGCAGCGGCCCGGGCACGGAGGGCAGAGGCCAAGG
CGGCCGCTGATGCCAAGAAGCAGAAGGAGCTGGAGGATGCCTACTGGAAGGACGACGACAAACACGTCAT
GAGGAAGGAGCAGCGCAAGGAGGAGAAGGAGAAGCGGCGCCTCGACCAGCTGGAACGTAAGAAGGAGACG
CAGCGCCTACTGGAGGAGGAGGACTCCAAGCTCAAGGGCGGCAAGGCGCCGCGGGTGGCCACGTCCAGCA
AGGTCACCCGGGCCCAGATCGAGGACACGCTGCGCCGAGACCATCAGCTCAGGGAGGCCCCGGACACAGC
CGAGAAAGCCAAGAGCCATCTGGAGGTGCCGCTGGAGGAGAACGTGAACCGCCGCGTGCTGGAGGAGGGC
AGCGTGGAGGCGCGCACCATCGAGGACGCCATTGCAGTGCTCAGCGTGGCGGAGGAGGCGGCCGACCGGC
ACCCAGAAAGACGCATGCGGGCAGCCTTCACAGCCTTTGAGGAAGCCCAGCTGCCGCGGCTCAAACAAGA
GAACCCCAACATGCGGCTGTCGCAGCTGAAACAGCTGCTCAAGAAGGAGTGGCTCCGCTCTCCTGACAAC
CCCATGAACCAGCGGGCCGTGCCCTTCAATGCCCCCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC200867 protein sequence
Red=Cloning site Green=Tags(s)

MPKKFQGENTKSAAARARRAEAKAAADAKKQKELEDAYWKDDDKHVMRKEQRKEEKEKRRLDQLERKKET
QRLLEEEDSKLKGGKAPRVATSSKVTRAQIEDTLRRDHQLREAPDTAEKAKSHLEVPLEENVNRRVLEEG
SVEARTIEDAIAVLSVAEEAADRHPERRMRAAFTAFEEAQLPRLKQENPNMRLSQLKQLLKKEWLRSPDN
PMNQRAVPFNAPK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138442
ORF Size 669 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138442.2, NP_612451.1
RefSeq Size 1066 bp
RefSeq ORF 672 bp
Locus ID 115098
UniProt ID Q96CT7, A0A024R7M8
Cytogenetics 19p13.11
MW 25.8 kDa
Summary Required for proper progression of late cytokinetic stages.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_138442" in other vectors (4)
SKU Description Size Price
RC200867L3 Lenti ORF clone of Human coiled-coil domain containing 124 (CCDC124), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC200867L4 Lenti ORF clone of Human coiled-coil domain containing 124 (CCDC124), transcript variant 1, mGFP tagged 10 ug
$600.00
RG200867 CCDC124 (tGFP-tagged) - Human coiled-coil domain containing 124 (CCDC124), transcript variant 1 10 ug
$489.00 $500.00
SC126174 CCDC124 (untagged)-Human coiled-coil domain containing 124 (CCDC124), transcript variant 1 10 ug
$300.00

Plasmid Map for RC200867

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Cloning scheme for RC200867

Circular map for RC200867

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