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RBED1 (ELMOD3) (NM_032213) Human Tagged ORF Clone

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SKU
RC200857
ELMOD3 (Myc-DDK-tagged)-Human ELMO/CED-12 domain containing 3 (ELMOD3), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RBED1
Synonyms DFNB88; LST3; RBED1; RBM29
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200857 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGAAAAATCTTGCTCTTTCCATAGTAAAGAAGAATTAAGAGATGGACAGGGTGAAAGATTGTCTG
CTGGATATTCTCCATCATATGACAAGGACAAGAGTGTTCTGGCTTTCAGAGGAATCCCTATCTCAGAGTT
GAAGAACCATGGCATTCTCCAGGCTCTGACCACAGAAGCTTATGAATGGGAGCCACGTGTTGTGAGTACA
GAGGTGGTCAGAGCCCAAGAAGAATGGGAAGCTGTGGACACCATCCAGCCAGAGACAGGGAGCCAAGCTA
GCTCAGAGCAGCCTGGGCAGCTAATCTCCTTCAGTGAGGCCCTGCAGCACTTCCAGACTGTGGACCTTTC
CCCCTTCAAGAAAAGAATCCAGCCAACTATTCGAAGGACTGGGCTCGCCGCCCTCCGACACTACCTCTTC
GGGCCTCCAAAGCTCCACCAGCGCCTTCGGGAAGAAAGGGACTTGGTCCTGACCATTGCTCAGTGTGGCC
TGGATAGCCAAGACCCAGTGCATGGCCGAGTCCTCCAGACCATCTATAAGAAGCTGACCGGCTCCAAGTT
TGACTGTGCCCTTCATGGAAACCACTGGGAGGACCTGGGCTTTCAGGGAGCGAATCCAGCCACAGACCTG
AGAGGCGCAGGCTTCCTTGCCCTCCTGCATCTGCTCTACCTGGTGATGGACTCAAAGACCTTGCCGATGG
CGCAGGAGATTTTCCGCCTGTCTCGTCACCACATCCAGCAATTCCCTTTCTGTTTGATGTCCGTGAACAT
CACCCACATTGCCATCCAGGCCTTGAGAGAGGAGTGTCTCTCCAGAGAGTGTAATCGGCAGCAGAAGGTC
ATCCCCGTGGTGAACAGCTTCTATGCCGCCACATTCCTCCATCTCGCACATGTCTGGAGGACACAGCGGA
AGACCATCTCAGACTCGGGCTTTGTCCTCAAAGGTGTGCTCTTTCTTCTGGGGAGGCCTAGGCTGAATGC
ACAGTGTCCCAGGTCCAGAGAGCCCAAGGTGGTTGCTAGACTGGTTTTGGCTGCAGTTCTTCCCCATCCA
CACTTTCTCAAATTCCAGCTTACCAAAATCTCCATCACCCACCCCCTGGAGTCTGCTAGTTCTCCTTTCT
CTGCCCTGACTGTTGCCCTTTTCTGGTCTTATACTTATGACAAGCATATATTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200857 protein sequence
Red=Cloning site Green=Tags(s)

MNEKSCSFHSKEELRDGQGERLSAGYSPSYDKDKSVLAFRGIPISELKNHGILQALTTEAYEWEPRVVST
EVVRAQEEWEAVDTIQPETGSQASSEQPGQLISFSEALQHFQTVDLSPFKKRIQPTIRRTGLAALRHYLF
GPPKLHQRLREERDLVLTIAQCGLDSQDPVHGRVLQTIYKKLTGSKFDCALHGNHWEDLGFQGANPATDL
RGAGFLALLHLLYLVMDSKTLPMAQEIFRLSRHHIQQFPFCLMSVNITHIAIQALREECLSRECNRQQKV
IPVVNSFYAATFLHLAHVWRTQRKTISDSGFVLKGVLFLLGRPRLNAQCPRSREPKVVARLVLAAVLPHP
HFLKFQLTKISITHPLESASSPFSALTVALFWSYTYDKHIF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032213
ORF Size 1173 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032213.3, NP_115589.2
RefSeq Size 2789 bp
RefSeq ORF 1176 bp
Locus ID 84173
Cytogenetics 2p11.2
Domains DUF609
MW 44.3 kDa
Summary This gene encodes a member of the engulfment and cell motility family of GTPase-activating proteins that regulate Arf GTPase proteins. Members of this family are defined by a conserved engulfment and cell motility domain. In rat cochlea, the encoded protein is found in stereocilia, kinocilia and cuticular plate of developing hair cells suggesting a function for this protein in cochlear sensory cells. An allelic variant of this family has been associated with autosomal recessive nonsyndromic deafness-88 in humans. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2016
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"NM_032213" in other vectors (4)
SKU Description Size Price
RC200857L3 Lenti ORF clone of Human ELMO/CED-12 domain containing 3 (ELMOD3), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC200857L4 Lenti ORF clone of Human ELMO/CED-12 domain containing 3 (ELMOD3), transcript variant 1, mGFP tagged 10 ug
$757.00
RG200857 ELMOD3 (tGFP-tagged) - Human ELMO/CED-12 domain containing 3 (ELMOD3), transcript variant 1 10 ug
$489.00 $657.00
SC128226 ELMOD3 (untagged)-Human ELMO/CED-12 domain containing 3 (ELMOD3), transcript variant 1 10 ug
$457.00

Plasmid Map for RC200857

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200857
Circular map for RC200857

file-alt Citations

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