FSTL3 (NM_005860) Human Tagged ORF Clone

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SKU
RC200811
FSTL3 (Myc-DDK-tagged)-Human follistatin-like 3 (secreted glycoprotein) (FSTL3)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FSTL3
Synonyms FLRG; FSRP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200811 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTCCCGGGGCGCCAGGGCCACTCTGGCCTCTGCCCTGGGGGGCCCTGGCTTGGGCCGTGGGCTTCG
TGAGCTCCATGGGCTCGGGGAACCCCGCGCCCGGTGGTGTTTGCTGGCTCCAGCAGGGCCAGGAGGCCAC
CTGCAGCCTGGTGCTCCAGACTGATGTCACCCGGGCCGAGTGCTGTGCCTCCGGCAACATTGACACCGCC
TGGTCCAACCTCACCCACCCGGGGAACAAGATCAACCTCCTCGGCTTCTTGGGCCTTGTCCACTGCCTTC
CCTGCAAAGATTCGTGCGACGGCGTGGAGTGCGGCCCGGGCAAGGCGTGCCGCATGCTGGGGGGCCGCCC
GCGCTGCGAGTGCGCGCCCGACTGCTCGGGGCTCCCGGCGCGGTTGCAGGTCTGCGGCTCAGACGGCGCC
ACCTACCGCGACGAGTGCGAGCTGCGCGCCGCGCGCTGCCGCGGCCACCCGGACCTGAGCGTCATGTACC
GGGGCCGCTGCCGCAAGTCCTGTGAGCACGTGGTGTGCCCGCGGCCACAGTCGTGCGTCGTGGACCAGAC
GGGCAGCGCCCACTGCGTGGTGTGTCGAGCGGCGCCCTGCCCTGTGCCCTCCAGCCCCGGCCAGGAGCTT
TGCGGCAACAACAACGTCACCTACATCTCCTCGTGCCACATGCGCCAGGCCACCTGCTTCCTGGGCCGCT
CCATCGGCGTGCGCCACGCGGGCAGCTGCGCAGGCACCCCTGAGGAGCCGCCAGGTGGTGAGTCTGCAGA
AGAGGAAGAGAACTTCGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC200811 protein sequence
Red=Cloning site Green=Tags(s)

MRPGAPGPLWPLPWGALAWAVGFVSSMGSGNPAPGGVCWLQQGQEATCSLVLQTDVTRAECCASGNIDTA
WSNLTHPGNKINLLGFLGLVHCLPCKDSCDGVECGPGKACRMLGGRPRCECAPDCSGLPARLQVCGSDGA
TYRDECELRAARCRGHPDLSVMYRGRCRKSCEHVVCPRPQSCVVDQTGSAHCVVCRAAPCPVPSSPGQEL
CGNNNVTYISSCHMRQATCFLGRSIGVRHAGSCAGTPEEPPGGESAEEEENFV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005860
ORF Size 789 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005860.3
RefSeq Size 2525 bp
RefSeq ORF 792 bp
Locus ID 10272
UniProt ID O95633, A0A024R1Y8
Cytogenetics 19p13.3
Domains FOLN, kazal
Protein Families Secreted Protein
MW 27.7 kDa
Summary Follistatin-like 3 is a secreted glycoprotein of the follistatin-module-protein family. It may have a role in leukemogenesis. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_005860" in other vectors (4)
SKU Description Size Price
RC200811L3 Lenti ORF clone of Human follistatin-like 3 (secreted glycoprotein) (FSTL3), Myc-DDK-tagged 10 ug
$600.00
RC200811L4 Lenti ORF clone of Human follistatin-like 3 (secreted glycoprotein) (FSTL3), mGFP tagged 10 ug
$600.00
RG200811 FSTL3 (tGFP-tagged) - Human follistatin-like 3 (secreted glycoprotein) (FSTL3) 10 ug
$489.00 $500.00
SC319496 FSTL3 (untagged)-Human follistatin-like 3 (secreted glycoprotein) (FSTL3) 10 ug
$300.00

Plasmid Map for RC200811

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Cloning scheme for RC200811

Circular map for RC200811

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