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CUEDC2 (NM_024040) Human Tagged ORF Clone

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SKU
RC200790
CUEDC2 (Myc-DDK-tagged)-Human CUE domain containing 2 (CUEDC2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CUEDC2
Synonyms bA18I14.5; C10orf66
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200790 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGGAGAGGATCGTCAGTGCAGCCCTCCTTGCCTTTGTCCAGACACACCTCCCGGAGGCCGACC
TCAGTGGCTTGGATGAGGTCATCTTCTCCTATGTGCTTGGGGTCCTGGAGGACCTGGGCCCCTCGGGCCC
ATCAGAGGAGAACTTCGATATGGAGGCTTTCACTGAGATGATGGAGGCCTATGTGCCTGGCTTCGCCCAC
ATCCCCAGGGGCACAATAGGGGACATGATGCAGAAGCTCTCAGGGCAGCTGAGCGATGCCAGGAACAAAG
AGAACCTGCAACCGCAGAGCTCTGGTGTCCAAGGTCAGGTGCCCATCTCCCCAGAGCCCCTGCAGCGGCC
CGAAATGCTCAAAGAAGAGACTAGGTCTTCGGCTGCTGCTGCTGCAGACACCCAAGATGAGGCAACTGGC
GCTGAGGAGGAGCTTCTGCCAGGGGTGGATGTACTCCTGGAGGTGTTCCCTACCTGTTCGGTGGAGCAGG
CCCAGTGGGTGCTGGCCAAAGCTCGGGGGGACTTGGAAGAAGCTGTGCAGATGCTGGTAGAGGGAAAGGA
AGAGGGGCCTGCAGCCTGGGAGGGCCCCAACCAGGACCTGCCCAGACGCCTCAGAGGCCCCCAAAAGGAT
GAGCTGAAGTCCTTCATCCTGCAGAAGTACATGATGGTGGATAGCGCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200790 protein sequence
Red=Cloning site Green=Tags(s)

MELERIVSAALLAFVQTHLPEADLSGLDEVIFSYVLGVLEDLGPSGPSEENFDMEAFTEMMEAYVPGFAH
IPRGTIGDMMQKLSGQLSDARNKENLQPQSSGVQGQVPISPEPLQRPEMLKEETRSSAAAAADTQDEATG
AEEELLPGVDVLLEVFPTCSVEQAQWVLAKARGDLEEAVQMLVEGKEEGPAAWEGPNQDLPRRLRGPQKD
ELKSFILQKYMMVDSA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024040
ORF Size 678 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024040.1, NP_076945.1
RefSeq Size 1203 bp
RefSeq ORF 864 bp
Locus ID 79004
UniProt ID Q9H467
Cytogenetics 10q24.32
Domains CUE
MW 24.7 kDa
Summary Down-regulates ESR1 protein levels through the ubiquitination-proteasome pathway, regardless of the presence of 17 beta-estradiol. Also involved in 17 beta-estradiol-induced ESR1 degradation. Controls PGR protein levels through a similar mechanism.UniProtKB/Swiss-Prot Function
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"NM_024040" in other vectors (5)
SKU Description Size Price
RC200790L3 Lenti ORF clone of Human CUE domain containing 2 (CUEDC2), Myc-DDK-tagged 10 ug
$600.00
RC200790L4 Lenti ORF clone of Human CUE domain containing 2 (CUEDC2), mGFP tagged 10 ug
$600.00
RG200790 CUEDC2 (tGFP-tagged) - Human CUE domain containing 2 (CUEDC2) 10 ug
$489.00 $500.00
SC317417 CUEDC2 (untagged)-Human CUE domain containing 2 (CUEDC2) 10 ug
$330.00
SC321584 CUEDC2 (untagged)-Human CUE domain containing 2 (CUEDC2) 10 ug
$300.00

Plasmid Map for RC200790

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200790
Circular map for RC200790

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