B9D2 (NM_030578) Human Tagged ORF Clone

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SKU
RC200762
B9D2 (Myc-DDK-tagged)-Human B9 protein domain 2 (B9D2)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol B9D2
Synonyms ICIS-1; JBTS34; MKS10; MKSR-2; MKSR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200762 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAGGTGCACGTGATCGGGCAGATCATGGGGGCCAGCGGTTTCTCGGAAAGTAGCCTCTTCTGCA
AGTGGGGCATTCACACAGGGGCGGCATGGAAGCTCCTGTCAGGCGTGCGGGAGGGCCAAACGCAAGTGGA
CACCCCGCAGATAGGGGACATGGCTTACTGGTCCCACCCCATCGACCTGCACTTCGCCACCAAAGGTCTT
CAAGGCTGGCCCCGGCTCCATTTCCAGGTGTGGTCCCAGGACAGCTTTGGCCGCTGCCAGCTTGCAGGCT
ATGGATTTTGCCATGTGCCCAGTAGCCCGGGCACCCACCAGCTGGCCTGCCCCACGTGGCGGCCCCTGGG
CAGTTGGCGAGAACAGTTGGCACGGGCTTTCGTGGGTGGTGGGCCGCAGCTGCTGCATGGGGACACCATC
TACAGTGGGGCCGACCGCTATCGCCTGCACACAGCTGCTGGTGGCACCGTGCACCTGGAGATCGGCCTGC
TGCTCCGCAACTTCGACCGCTACGGCGTGGAGTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC200762 protein sequence
Red=Cloning site Green=Tags(s)

MAEVHVIGQIMGASGFSESSLFCKWGIHTGAAWKLLSGVREGQTQVDTPQIGDMAYWSHPIDLHFATKGL
QGWPRLHFQVWSQDSFGRCQLAGYGFCHVPSSPGTHQLACPTWRPLGSWREQLARAFVGGGPQLLHGDTI
YSGADRYRLHTAAGGTVHLEIGLLLRNFDRYGVEC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030578
ORF Size 525 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030578.2, NP_085055.1
RefSeq Size 1049 bp
RefSeq ORF 528 bp
Locus ID 80776
UniProt ID Q9BPU9
Cytogenetics 19q13.2
MW 19.3 kDa
Summary This gene encodes a B9 domain protein, which are exclusively found in ciliated organisms. The gene is upregulated during mucociliary differentiation, and the encoded protein localizes to basal bodies and cilia. Disrupting expression of this gene results in ciliogenesis defects. provided by RefSeq, Oct 2009
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Documents
Resources
"NM_030578" in other vectors (6)
SKU Description Size Price
RC200762L1 Lenti ORF clone of Human B9 protein domain 2 (B9D2), Myc-DDK-tagged 10 ug
$600.00
RC200762L2 Lenti ORF clone of Human B9 protein domain 2 (B9D2), mGFP tagged 10 ug
$600.00
RC200762L3 Lenti ORF clone of Human B9 protein domain 2 (B9D2), Myc-DDK-tagged 10 ug
$600.00
RC200762L4 Lenti ORF clone of Human B9 protein domain 2 (B9D2), mGFP tagged 10 ug
$600.00
RG200762 B9D2 (tGFP-tagged) - Human B9 protein domain 2 (B9D2) 10 ug
$489.00 $500.00
SC122988 B9D2 (untagged)-Human B9 protein domain 2 (B9D2) 10 ug
$300.00

Plasmid Map for RC200762

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Cloning scheme for RC200762

Circular map for RC200762

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