Caspase 9 (CASP9) (NM_001229) Human Tagged ORF Clone
SKU
RC200635
CASP9 (Myc-DDK-tagged)-Human caspase 9, apoptosis-related cysteine peptidase (CASP9), transcript variant alpha
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Caspase 9 |
| Synonyms | APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001229 |
| ORF Size | 1248 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001229.5 |
| RefSeq Size | 2034 bp |
| RefSeq ORF | 1251 bp |
| Locus ID | 842 |
| UniProt ID | P55211 |
| Cytogenetics | 1p36.21 |
| Domains | CARD, CASc, Peptidase_C14 |
| Protein Families | Druggable Genome, Protease, Stem cell - Pluripotency |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Endometrial cancer, Huntington's disease, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Parkinson's disease, Pathways in cancer, Prostate cancer, Small cell lung cancer, VEGF signaling pathway, Viral myocarditis |
| MW | 46.1 kDa |
| Summary | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. provided by RefSeq, May 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001229" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200635L1 | Lenti ORF clone of Human caspase 9, apoptosis-related cysteine peptidase (CASP9), transcript variant alpha, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC200635L2 | Lenti ORF clone of Human caspase 9, apoptosis-related cysteine peptidase (CASP9), transcript variant alpha, mGFP tagged | 10 ug |
$757.00
|
|
| RC200635L3 | Lenti ORF clone of Human caspase 9, apoptosis-related cysteine peptidase (CASP9), transcript variant alpha, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC200635L4 | Lenti ORF clone of Human caspase 9, apoptosis-related cysteine peptidase (CASP9), transcript variant alpha, mGFP tagged | 10 ug |
$757.00
|
|
| RG200635 | CASP9 (tGFP-tagged) - Human caspase 9, apoptosis-related cysteine peptidase (CASP9), transcript variant alpha | 10 ug |
$489.00
$657.00
|
|
| SC119362 | CASP9 (untagged)-Human caspase 9, apoptosis-related cysteine peptidase (CASP9), transcript variant alpha | 10 ug |
$457.00
|
|
Citations
A Fragment-Derived Clinical Candidate for Antagonism of X-Linked and Cellular Inhibitor of Apoptosis Proteins: 1-(6-[(4-Fluorophenyl)methyl]-5-(hydroxymethyl)-3,3-dimethyl-1 H,2 H,3 H-pyrrolo[3,2- b]pyridin-1-yl)-2-[(2 R,5 R)-5-methyl-2-([(3R)-3-methylmorpholin-4-yl]methyl)piperazin-1-yl]ethan-1-one (ASTX660)
Johnson, Ahn, Buck et al
J. Med. Chem. (2018) 61 (16), 7314-7329 DOI: 10.1021/acs.jmedchem.8b00900
Johnson, Ahn, Buck et al
J. Med. Chem. (2018) 61 (16), 7314-7329 DOI: 10.1021/acs.jmedchem.8b00900
AI Summary: OriGene products contributed to this study by providing the full-length FLAG-tagged XIAP expression construct and the full-length untagged caspase-9 construct, which were used to engineer HEK293 cell lines for evaluating the activity of the clinical candidate ASTX660.
Snippet: An engineered HEK293 cell line was generated by transfecting the HEK293 cell line (ECACC, Salisbury, UK) with a full-length FLAG-tagged XIAP expression construct [Origene Technologies Inc., Rockville, USA] and a full-length untagged caspase-9 construct [Origene Technologies, Inc., Rockville, USA]
Genetic screening and functional analysis of CASP9 mutations in a Chinese cohort with neural tube defects
Liu, Zhang, Jiang et al
CNS Neurosci Ther (2018) 24 (5), 394-403 DOI: 10.1111/cns.12797
Liu, Zhang, Jiang et al
CNS Neurosci Ther (2018) 24 (5), 394-403 DOI: 10.1111/cns.12797
AI Summary: OriGene Technologies contributed to this study by providing synthesized wild-type and variant human CASP9 open-reading frames (ORFs) with Myc-DDK tags, which were cloned into the pCMV6-AC expression vector for functional assays.
Snippet: 2.3 Plasmids, cell culture, and transfection. Wild-type (WT) human CASP9 and variant open-reading frames (ORFs), labeled with the Myc-DDK tag at their C-termini, were synthesized by OriGene Technologies and cloned into the expression vector pCMV6-AC
Showing 1-2 of 2 papers.
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