NQO1 (NM_000903) Human Tagged ORF Clone
SKU
RC200620
NQO1 (Myc-DDK-tagged)-Human NAD(P)H dehydrogenase, quinone 1 (NQO1), transcript variant 1
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | NQO1 |
| Synonyms | DHQU; DIA4; DTD; NMOR1; NMORI; QR1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000903 |
| ORF Size | 822 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000903.3 |
| RefSeq Size | 2601 bp |
| RefSeq ORF | 825 bp |
| Locus ID | 1728 |
| UniProt ID | P15559 |
| Cytogenetics | 16q22.1 |
| Domains | Flavodoxin_2 |
| Protein Families | Druggable Genome |
| MW | 30.9 kDa |
| Summary | This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000903" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200620L1 | Lenti ORF clone of Human NAD(P)H dehydrogenase, quinone 1 (NQO1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC200620L2 | Lenti ORF clone of Human NAD(P)H dehydrogenase, quinone 1 (NQO1), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RC200620L3 | Lenti ORF clone of Human NAD(P)H dehydrogenase, quinone 1 (NQO1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC200620L4 | Lenti ORF clone of Human NAD(P)H dehydrogenase, quinone 1 (NQO1), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RG200620 | NQO1 (tGFP-tagged) - Human NAD(P)H dehydrogenase, quinone 1 (NQO1), transcript variant 1 | 10 ug |
$489.00
$500.00
|
|
| SC119599 | NQO1 (untagged)-Human NAD(P)H dehydrogenase, quinone 1 (NQO1), transcript variant 1 | 10 ug |
$300.00
|
|
Citations
Loss of NQO1 generates genotoxic estrogen-DNA adducts in Fuchs Endothelial Corneal Dystrophy
Miyajima, Melangath, Zhu et al
Free Radic. Biol. Med. (2019) 147, 69-79 DOI: 10.1016/j.freeradbiomed.2019.12.014
Miyajima, Melangath, Zhu et al
Free Radic. Biol. Med. (2019) 147, 69-79 DOI: 10.1016/j.freeradbiomed.2019.12.014
Cited by 33
AI Summary: OriGene's pCMV6-NQO1-myc plasmid was used to overexpress NQO1 in human corneal endothelial cells, enabling the study to demonstrate that increased NQO1 levels can reduce oxidative DNA damage and cell loss associated with Fuchs Endothelial Corneal Dystrophy.
Snippet: HCEnC-21T cells were seeded in 12-well plate and transiently transfected with myc-tagged NQO1 plasmid (pCMV6-NQO1-myc, Origene, Rockville, MD) and control plasmid (pCMV6-myc) at 1.5 μg/well with 3 μL Lipofectamine 2000 (Thermo Fisher Scientific) in OptiMEM;- HCEnC-21T cells were seeded in 12-well plate and transiently transfected with myc-tagged NQO1 plasmid (pCMV6-NQO1-myc, Origene, Rockville, MD) and control plasmid (pCMV6-myc) at 1.5 μg/well with 3 μL Lipofectamine 2000 (Thermo Fisher Scientific) in OptiMEM;
Bioactivation of napabucasin triggers reactive oxygen species-mediated cancer cell death
Froeling, Mosur Swamynathan, Deschênes et al
Clin. Cancer Res. (2019) 25 (23), 7162-7174 DOI: 10.1158/1078-0432.CCR-19-0302
Froeling, Mosur Swamynathan, Deschênes et al
Clin. Cancer Res. (2019) 25 (23), 7162-7174 DOI: 10.1158/1078-0432.CCR-19-0302
AI Summary: OriGene’s NQO1 cDNA (catalog number RC200620) was used to transfect and restore NQO1 expression in pancreatic cancer cells, enabling the study of napabucasin’s bioactivation and its role in ROS-mediated cancer cell death.
Snippet: and complete NQO1 knock-out was verified by Western blot. For expression of NQO1 in Panc1 140 cells, NQO1 was introduced by transfection of cDNA (Origene, RC200620) using XtremeGENE 141 9 (Roche, 06365787001) according to manufacturer instructions;- and complete NQO1 knock-out was verified by Western blot. For expression of NQO1 in Panc1 140 cells, NQO1 was introduced by transfection of cDNA (Origene, RC200620) using XtremeGENE 141 9 (Roche, 06365787001) according to manufacturer instructions
Evidence of activation of the Nrf2 pathway in multiple sclerosis patients treated with delayed-release dimethyl fumarate in the Phase 3 DEFINE and CONFIRM studies
Gopal, Mikulskis, Gold et al
Mult. Scler. (2017)
Gopal, Mikulskis, Gold et al
Mult. Scler. (2017)
NAD(P)H-dependent Quinone Oxidoreductase 1 (NQO1) and Cytochrome P450 Oxidoreductase (CYP450OR) differentially regulate menadione-mediated alterations in redox status, survival and metabolism in pancreatic β-cells
Gray, Karandrea, Burgos et al
Toxicol. Lett. (2016)
Gray, Karandrea, Burgos et al
Toxicol. Lett. (2016)
Cited by 23
AI Summary: OriGene products contributed to this study by providing plasmid constructs encoding human NQO1 and CYP450OR genes, which were used to generate recombinant adenoviruses for overexpression experiments in pancreatic β-cells.
Plasma membrane electron transport in pancreatic ß-cells is mediated in part by NQO1
Gray, Eisen, Cline et al
Am J Physiol Endocrinol Metab (2011)
Gray, Eisen, Cline et al
Am J Physiol Endocrinol Metab (2011)
Showing 1-5 of 5 papers.
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