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PRICKLE3 (NM_006150) Human Tagged ORF Clone

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SKU
RC200553
PRICKLE3 (Myc-DDK-tagged)-Human prickle homolog 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $572.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PRICKLE3
Synonyms LMO6; LOAM; Pk3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200553 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCGCGCGTGGGTCCCGGAGGCGCCGCTCCGGGCGTGCGCCTCCAGAGGCAGAGGACCCAGACCGGG
GCCAGCCCTGCAACTCCTGTAGGGAGCAGTGCCCTGGCTTCCTGCTCCACGGCTGGAGAAAGATCTGCCA
GCATTGCAAATGCCCGCGGGAGGAGCATGCAGTGCACGCGGTGCCTGTGGACCTGGAACGCATCATGTGT
CGGCTAATCTCGGACTTCCAGCGCCACTCCATCTCCGACGACGACTCAGGCTGTGCATCGGAGGAGTATG
CCTGGGTGCCCCCAGGCCTTAAGCCGGAGCAGGTATATCAATTTTTCAGCTGCCTCCCAGAGGACAAGGT
CCCCTACGTCAACAGTCCTGGGGAGAAATACAGGATCAAGCAGCTGCTGCACCAGCTGCCCCCACACGAC
AGTGAGGCACAGTACTGCACAGCACTGGAAGAGGAGGAAAAGAAAGAGCTCCGAGCCTTTAGCCAGCAGC
GGAAGCGGGAGAATCTGGGGCGTGGCATCGTGCGCATCTTCCCGGTGACCATCACTGGGGCCATCTGTGA
GGAGTGCGGAAAGCAGATTGGAGGTGGGGACATCGCAGTGTTTGCCAGCCGTGCAGGCCTGGGTGCCTGC
TGGCACCCACAGTGCTTCGTGTGTACCACGTGCCAGGAACTGCTGGTTGACCTCATCTACTTCTACCATG
TTGGCAAGGTCTACTGCGGGCGTCACCATGCCGAATGCCTGCGTCCACGCTGCCAAGCCTGTGACGAGAT
CATCTTCTCCCCTGAGTGCACGGAGGCTGAGGGCCGCCACTGGCACATGGATCACTTCTGCTGCTTTGAG
TGTGAAGCTTCACTAGGAGGGCAGCGCTATGTCATGCGTCAGAGCCGCCCCCACTGCTGCGCCTGCTACG
AGGCCCGCCACGCGGAGTACTGTGATGGCTGTGGGGAGCACATCGGCCTGGACCAAGGCCAGATGGCTTA
CGAGGGCCAGCACTGGCATGCCTCAGACCGCTGCTTCTGCTGTAGTCGCTGTGGGCGGGCCCTGCTGGGC
CGCCCATTCCTGCCACGCCGAGGCCTAATCTTCTGCTCTCGAGCCTGCAGCCTTGGGTCCGAGCCCACAG
CTCCAGGGCCGAGCCGCCGCAGCTGGAGTGCCGGCCCTGTCACAGCCCCACTTGCAGCCTCCACAGCCTC
TTTCTCTGCTGTGAAGGGGGCATCAGAGACCACCACCAAAGGCACCAGCACAGAGTTAGCGCCAGCTACA
GGCCCTGAGGAGCCCTCCCGCTTTCTGAGAGGGGCTCCCCACCGCCACTCCATGCCGGAACTGGGGCTCC
GCAGTGTCCCCGAGCCGCCCCCAGAGTCCCCCGGCCAGCCTAACCTGCGCCCAGATGATAGTGCCTTCGG
TCGTCAGAGCACCCCACGCGTCAGCTTCCGCGACCCTCTGGTGTCTGAAGGAGGCCCGCGCCGGACCCTG
AGTGCACCCCCGGCCCAGCGCCGCAGGCCACGCAGTCCCCCACCCAGGGCCCCCAGCCGTCGCCGCCACC
ATCATCATAATCACCATCACCATCACAACCGCCACCCAAGCAGACGTCGCCACTATCAATGTGACGCGGG
ATCAGGGTCAGACTCGGAATCTTGCTCCAGCTCGCCCTCCAGTTCCAGTTCCGAATCATCAGAGGATGAT
GGCTTCTTCCTAGGAGAGCGCATCCCTCTGCCCCCGCATTTGTGCAGGCCCATGCCTGCTCAGGACACTG
CAATGGAGACCTTCAACTCCCCATCTTTATCGCTCCCCAGGGACTCTCGCGCAGGGATGCCTCGTCAGGC
CCGAGACAAGAACTGCATCGTGGCT

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200553 protein sequence
Red=Cloning site Green=Tags(s)

MFARGSRRRRSGRAPPEAEDPDRGQPCNSCREQCPGFLLHGWRKICQHCKCPREEHAVHAVPVDLERIMC
RLISDFQRHSISDDDSGCASEEYAWVPPGLKPEQVYQFFSCLPEDKVPYVNSPGEKYRIKQLLHQLPPHD
SEAQYCTALEEEEKKELRAFSQQRKRENLGRGIVRIFPVTITGAICEECGKQIGGGDIAVFASRAGLGAC
WHPQCFVCTTCQELLVDLIYFYHVGKVYCGRHHAECLRPRCQACDEIIFSPECTEAEGRHWHMDHFCCFE
CEASLGGQRYVMRQSRPHCCACYEARHAEYCDGCGEHIGLDQGQMAYEGQHWHASDRCFCCSRCGRALLG
RPFLPRRGLIFCSRACSLGSEPTAPGPSRRSWSAGPVTAPLAASTASFSAVKGASETTTKGTSTELAPAT
GPEEPSRFLRGAPHRHSMPELGLRSVPEPPPESPGQPNLRPDDSAFGRQSTPRVSFRDPLVSEGGPRRTL
SAPPAQRRRPRSPPPRAPSRRRHHHHNHHHHHNRHPSRRRHYQCDAGSGSDSESCSSSPSSSSSESSEDD
GFFLGERIPLPPHLCRPMPAQDTAMETFNSPSLSLPRDSRAGMPRQARDKNCIVA

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_006150
ORF Size 1845 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006150.5
RefSeq Size 1990 bp
RefSeq ORF 1848 bp
Locus ID 4007
UniProt ID O43900, A0A024QYW5, B7Z8D2, B7Z5U0
Cytogenetics Xp11.23
Domains LIM
MW 68.6 kDa
Summary LIM domain only 6 is a three LIM domain-containing protein. The LIM domain is a cysteine-rich sequence motif that binds zinc atoms to form a specific protein-binding interface for protein-protein interactions. provided by RefSeq, Jul 2008
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Documents
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"NM_006150" in other vectors (7)
SKU Description Size Price
RC200553L1 Lenti ORF clone of Human prickle homolog 3 (PRICKLE3), Myc-DDK-tagged 10 ug
$872.00
RC200553L2 Lenti ORF clone of Human prickle homolog 3 (PRICKLE3), mGFP tagged 10 ug
$872.00
RC200553L3 Lenti ORF clone of Human prickle homolog 3 (PRICKLE3), Myc-DDK-tagged 10 ug
$872.00
RC200553L4 Lenti ORF clone of Human prickle homolog 3 (PRICKLE3), mGFP tagged 10 ug
$872.00
RG200553 PRICKLE3 (tGFP-tagged) - Human prickle homolog 3 10 ug
$489.00 $772.00
SC116277 PRICKLE3 (untagged)-Human prickle homolog 3 10 ug
$573.00
SC320564 PRICKLE3 (untagged)-Human prickle homolog 3 10 ug
$573.00

Plasmid Map for RC200553

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200553
Circular map for RC200553

file-alt Citations

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