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BTG1 (NM_001731) Human Tagged ORF Clone

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SKU
RC200498
BTG1 (Myc-DDK-tagged)-Human B-cell translocation gene 1, anti-proliferative (BTG1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BTG1
Synonyms APRO2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200498 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATCCCTTCTACACCCGGGCCGCCACCATGATAGGCGAGATCGCCGCCGCCGTGTCCTTCATCTCCA
AGTTTCTCCGCACCAAGGGGCTCACGAGCGAGCGACAGCTGCAGACCTTCAGCCAGAGCCTGCAGGAGCT
GCTGGCAGAACATTATAAACATCACTGGTTCCCAGAAAAGCCATGCAAGGGATCGGGTTACCGTTGTATT
CGCATCAACCATAAAATGGATCCTCTGATTGGACAGGCAGCACAGCGGATTGGACTGAGCAGTCAGGAGC
TGTTCAGGCTTCTCCCAAGTGAACTCACACTCTGGGTTGACCCCTATGAAGTGTCCTACAGAATTGGAGA
GGATGGCTCCATCTGTGTGCTGTATGAAGCCTCACCAGCAGGAGGTAGCACTCAAAACAGCACCAACGTG
CAAATGGTAGACAGCCGAATCAGCTGTAAGGAGGAACTTCTCTTGGGCAGAACGAGCCCTTCCAAAAACT
ACAATATGATGACTGTATCAGGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200498 protein sequence
Red=Cloning site Green=Tags(s)

MHPFYTRAATMIGEIAAAVSFISKFLRTKGLTSERQLQTFSQSLQELLAEHYKHHWFPEKPCKGSGYRCI
RINHKMDPLIGQAAQRIGLSSQELFRLLPSELTLWVDPYEVSYRIGEDGSICVLYEASPAGGSTQNSTNV
QMVDSRISCKEELLLGRTSPSKNYNMMTVSG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001731
ORF Size 513 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001731.3
RefSeq Size 4704 bp
RefSeq ORF 516 bp
Locus ID 694
UniProt ID P62324
Cytogenetics 12q21.33
Domains Anti_proliferat
Protein Families Transcription Factors
MW 19.2 kDa
Summary This gene is a member of an anti-proliferative gene family that regulates cell growth and differentiation. Expression of this gene is highest in the G0/G1 phases of the cell cycle and downregulated when cells progressed through G1. The encoded protein interacts with several nuclear receptors, and functions as a coactivator of cell differentiation. This locus has been shown to be involved in a t(8;12)(q24;q22) chromosomal translocation in a case of B-cell chronic lymphocytic leukemia. provided by RefSeq, Oct 2008
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"NM_001731" in other vectors (6)
SKU Description Size Price
RC200498L1 Lenti ORF clone of Human B-cell translocation gene 1, anti-proliferative (BTG1), Myc-DDK-tagged 10 ug
$600.00
RC200498L2 Lenti ORF clone of Human B-cell translocation gene 1, anti-proliferative (BTG1), mGFP tagged 10 ug
$600.00
RC200498L3 Lenti ORF clone of Human B-cell translocation gene 1, anti-proliferative (BTG1), Myc-DDK-tagged 10 ug
$600.00
RC200498L4 Lenti ORF clone of Human B-cell translocation gene 1, anti-proliferative (BTG1), mGFP tagged 10 ug
$600.00
RG200498 BTG1 (tGFP-tagged) - Human B-cell translocation gene 1, anti-proliferative (BTG1) 10 ug
$489.00 $500.00
SC119069 BTG1 (untagged)-Human B-cell translocation gene 1, anti-proliferative (BTG1) 10 ug
$300.00

Plasmid Map for RC200498

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200498
Circular map for RC200498

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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