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DGCR6L (NM_033257) Human Tagged ORF Clone

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SKU
RC200317
DGCR6L (Myc-DDK-tagged)-Human DiGeorge syndrome critical region gene 6-like (DGCR6L)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DGCR6L
Synonyms DGCR6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200317 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGCTACGCGGCCGCCTTGGAGGAGGTGGCGGACGGTGCCCGGCAGCAGGAGCGACACTACCAGT
TGCTGTCGGCGCTACAGAGCCTGGTGAAGGAGTTGCCCAGCTCTTTCCAGCAGCGCCTGTCCTACACCAC
GCTCAGCGACCTGGCCCTGGCGCTTCTCGACGGCACCGTGTTCGAAATCGTGCAGGGGCTACTGGAGATC
CAGCACCTCACCGAAAAGAGCCTGTACAACCAGCGCCTGCGCCTACAGAACGAGCACCGAGTGCTCAGGC
AGGCGCTGCGGCAGAAGCACCAGGAAGCCCAGCAGGCCTGCCGGCCCCACAACCTGCCTGTGGTTCAGGC
GGCTCAGCAGCGAGAACTAGAGGCCGTGGAACACCGGATCCGTGAGGAGCAGCGGGCGATGGACCAGAAG
ATCATCCTGGAGCTGGACCGGAAGGTGGCTGACCAGCAGAGCACACTGGAGAAGGCGGGGGTGGCTGGCT
TCTACGTGACCACCAACCCACAGGAGCTGATGCTGCAGATGAACCTGCTGGAACTCATCCGAAAGCTGCA
GCAGAGGGGCTGCCGGGCAGGGAATGCAGCCCTGGGACTGGGAGGTCCCTGGCAGTCGCCTGCTGCCCAG
TGTGACCAGAAAGGCAGCCCTGTCCCACCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200317 protein sequence
Red=Cloning site Green=Tags(s)

MERYAAALEEVADGARQQERHYQLLSALQSLVKELPSSFQQRLSYTTLSDLALALLDGTVFEIVQGLLEI
QHLTEKSLYNQRLRLQNEHRVLRQALRQKHQEAQQACRPHNLPVVQAAQQRELEAVEHRIREEQRAMDQK
IILELDRKVADQQSTLEKAGVAGFYVTTNPQELMLQMNLLELIRKLQQRGCRAGNAALGLGGPWQSPAAQ
CDQKGSPVPP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033257
ORF Size 660 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033257.4
RefSeq Size 1217 bp
RefSeq ORF 663 bp
Locus ID 85359
UniProt ID Q9BY27
Cytogenetics 22q11.21
Protein Families Druggable Genome, Transcription Factors
MW 24.9 kDa
Summary This gene, the result of a duplication at this locus, is one of two functional genes encoding nearly identical proteins that have similar expression patterns. The product of this gene is a protein that shares homology with the Drosophila gonadal protein, expressed in gonadal tissues and germ cells, and with the human laminin gamma-1 chain that functions in cell attachment and migration. This gene is located in a region of chromosome 22 implicated in the DiGeorge syndrome, one facet of a broader collection of anomalies referred to as the CATCH 22 syndrome. provided by RefSeq, Jul 2008
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Documents
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"NM_033257" in other vectors (7)
SKU Description Size Price
RC200317L1 Lenti ORF clone of Human DiGeorge syndrome critical region gene 6-like (DGCR6L), Myc-DDK-tagged 10 ug
$600.00
RC200317L2 Lenti ORF clone of Human DiGeorge syndrome critical region gene 6-like (DGCR6L), mGFP tagged 10 ug
$600.00
RC200317L3 Lenti ORF clone of Human DiGeorge syndrome critical region gene 6-like (DGCR6L), Myc-DDK-tagged 10 ug
$600.00
RC200317L4 Lenti ORF clone of Human DiGeorge syndrome critical region gene 6-like (DGCR6L), mGFP tagged 10 ug
$600.00
RG200317 DGCR6L (tGFP-tagged) - Human DiGeorge syndrome critical region gene 6-like (DGCR6L) 10 ug
$489.00 $500.00
SC126909 DGCR6L (untagged)-Human DiGeorge syndrome critical region gene 6-like (DGCR6L) 10 ug
$300.00
SC320652 DGCR6L (untagged)-Human DiGeorge syndrome critical region gene 6-like (DGCR6L) 10 ug
$300.00

Plasmid Map for RC200317

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200317
Circular map for RC200317

file-alt Citations

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