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GPAA1 (NM_003801) Human Tagged ORF Clone

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SKU
RC200271
GPAA1 (Myc-DDK-tagged)-Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $578.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GPAA1
Synonyms GAA1; GPIBD15; hGAA1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200271 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCTCCTGTCGGACCCGGTTCGCCGGCGCGCGCTCGCCCGCCTAGTGCTGCGCCTCAACGCGCCGT
TGTGCGTGCTGAGCTACGTGGCGGGCATCGCCTGGTTCTTGGCGCTGGTTTTCCCGCCGCTGACCCAGCG
CACTTACATGTCGGAGAACGCCATGGGCTCCACCATGGTGGAGGAGCAGTTTGCGGGCGGAGACCGTGCC
CGGGCTTTTGCCCGGGACTTCGCCGCCCACCGCAAGAAGTCGGGGGCTCTGCCAGTGGCCTGGCTTGAAC
GGACGATGCGGTCAGTAGGGCTGGAGGTCTACACGCAGAGTTTCTCCCGGAAACTGCCCTTCCCAGATGA
GACCCACGAGCGCTATATGGTGTCGGGCACCAACGTGTACGGCATCCTGCGGGCCCCGCGTGCTGCCAGC
ACCGAGTCGCTTGTGCTCACCGTGCCCTGTGGCTCTGACTCTACCAACAGCCAGGCTGTGGGGCTGCTGC
TGGCACTGGCTGCCCACTTCCGGGGGCAGATTTATTGGGCCAAAGATATCGTCTTCCTGGTAACAGAACA
TGACCTTCTGGGCACTGAGGCTTGGCTTGAAGCCTACCACGATGTCAATGTCACTGGCATGCAGTCGTCT
CCCCTGCAGGGCCGAGCTGGGGCCATTCAGGCAGCCGTGGCCCTGGAGCTGAGCAGTGATGTGGTCACCA
GCCTCGATGTGGCCGTGGAGGGGCTTAACGGGCAGCTGCCCAACCTTGACCTGCTCAATCTCTTCCAGAC
CTTCTGCCAGAAAGGGGGCCTGTTGTGCACGCTTCAGGGCAAGCTGCAGCCCGAGGACTGGACATCATTG
GATGGACCGCTGCAGGGCCTGCAGACACTGCTGCTCATGGTTCTGCGGCAGGCCTCCGGCCGCCCCCACG
GCTCCCATGGCCTCTTCCTGCGCTACCGTGTGGAGGCCCTAACCCTGCGTGGCATCAATAGCTTCCGCCA
GTACAAGTATGACCTGGTGGCAGTGGGCAAGGCTTTGGAGGGCATGTTCCGCAAGCTCAACCACCTCCTG
GAGCGCCTGCACCAGTCCTTCTTCCTCTACTTGCTCCCCGGCCTCTCCCGCTTCGTCTCCATCGGCCTCT
ACATGCCCGCTGTCGGCTTCTTGCTCCTGGTCCTTGGTCTCAAGGCTCTGGAACTGTGGATGCAGCTGCA
TGAGGCTGGAATGGGCCTTGAGGAGCCCGGGGGTGCCCCTGGCCCCAGTGTACCCCTTCCCCCATCACAG
GGTGTGGGGCTGGCCTCGCTCGTGGCACCTCTGCTGATCTCACAGGCCATGGGACTGGCCCTCTATGTCC
TGCCAGTGCTGGGCCAACACGTTGCCACCCAGCACTTCCCAGTGGCAGAGGCTGAGGCTGTGGTGCTGAC
ACTGCTGGCGATTTATGCAGCTGGCCTGGCCCTGCCCCACAATACCCACCGGGTGGTAAGCACACAGGCC
CCAGACAGGGGCTGGATGGCACTGAAGCTGGTAGCCCTGATCTACCTAGCACTGCAGCTGGGCTGCATCG
CCCTCACCAACTTCTCACTGGGCTTCCTGCTGGCCACCACCATGGTGCCCACTGCTGCGCTTGCCAAGCC
TCATGGGCCCCGGACCCTCTATGCTGCCCTGCTGGTGCTGACCAGCCCGGCAGCCACGCTCCTTGGCAGC
CTGTTCCTGTGGCGGGAGCTGCAGGAGGCGCCACTGTCACTGGCCGAGGGCTGGCAGCTCTTCCTGGCAG
CGCTAGCCCAGGGTGTGCTGGAGCACCACACCTACGGCGCCCTGCTCTTCCCACTGCTGTCCCTGGGCCT
CTACCCCTGCTGGCTGCTTTTCTGGAATGTGCTCTTCTGGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200271 protein sequence
Red=Cloning site Green=Tags(s)

MGLLSDPVRRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGSTMVEEQFAGGDRA
RAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTNVYGILRAPRAAS
TESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVFLVTEHDLLGTEAWLEAYHDVNVTGMQSS
PLQGRAGAIQAAVALELSSDVVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSL
DGPLQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLL
ERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQ
GVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQA
PDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGPRTLYAALLVLTSPAATLLGS
LFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFWNVLFWK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003801
ORF Size 1863 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003801.4
RefSeq Size 2105 bp
RefSeq ORF 1866 bp
Locus ID 8733
UniProt ID O43292
Cytogenetics 8q24.3
Domains Gaa1
Protein Families Transmembrane
Protein Pathways Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
MW 67.6 kDa
Summary Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains. provided by RefSeq, Jul 2008
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"NM_003801" in other vectors (6)
SKU Description Size Price
RC200271L1 Lenti ORF clone of Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1), Myc-DDK-tagged 10 ug
$878.00
RC200271L2 Lenti ORF clone of Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1), mGFP tagged 10 ug
$878.00
RC200271L3 Lenti ORF clone of Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1), Myc-DDK-tagged 10 ug
$878.00
RC200271L4 Lenti ORF clone of Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1), mGFP tagged 10 ug
$878.00
RG200271 GPAA1 (tGFP-tagged) - Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1) 10 ug
$489.00 $778.00
SC319411 GPAA1 (untagged)-Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1) 10 ug
$579.00

Plasmid Map for RC200271

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200271
Circular map for RC200271

file-alt Citations

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