HSP70-1A (HSPA1A) (NM_005345) Human Tagged ORF Clone
$895.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | HSP70-1A |
| Synonyms | HEL-S-103; HSP70-1; HSP70-1A; HSP70-2; HSP70.1; HSP70.2; HSP70I; HSP72; HSPA1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005345 |
| ORF Size | 1923 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005345.6 |
| RefSeq Size | 2383 bp |
| RefSeq ORF | 1926 bp |
| Locus ID | 3303 |
| UniProt ID | P08107 |
| Cytogenetics | 6p21.33 |
| Domains | HSP70 |
| Protein Pathways | Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Prion diseases, Spliceosome |
| MW | 69.9 kDa |
| Summary | This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005345" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200270L1 | Lenti ORF clone of Human heat shock 70kDa protein 1A (HSPA1A), Myc-DDK-tagged | 10 ug |
$1,195.00
|
|
| RC200270L2 | Lenti ORF clone of Human heat shock 70kDa protein 1A (HSPA1A), mGFP tagged | 10 ug |
$1,195.00
|
|
| RC200270L3 | Lenti ORF clone of Human heat shock 70kDa protein 1A (HSPA1A), Myc-DDK-tagged | 10 ug |
$1,195.00
|
|
| RC200270L4 | Lenti ORF clone of Human heat shock 70kDa protein 1A (HSPA1A), mGFP tagged | 10 ug |
$1,195.00
|
|
| RG200270 | HSPA1A (tGFP-tagged) - Human heat shock 70kDa protein 1A (HSPA1A) | 10 ug |
$1,095.00
|
|
| SC116766 | HSPA1A (untagged)-Human heat shock 70kDa protein 1A (HSPA1A) | 10 ug |
$896.00
|
|
| SC320646 | HSPA1A (untagged)-Human heat shock 70kDa protein 1A (HSPA1A) | 10 ug |
$896.00
|
|
Citations
Extracellular Heat Shock Protein 70 Increases the Glucocorticoid Receptor and Dual-Specificity Phosphatase 1 via Toll-like Receptor 4 and Attenuates Inflammation in Airway Epithelial Cells
Zhou, Fang, Tamm et al
Int J Mol Sci (2023) 24 (14) DOI: 10.3390/ijms241411700
Zhou, Fang, Tamm et al
Int J Mol Sci (2023) 24 (14) DOI: 10.3390/ijms241411700
Cited by 14
AI Summary: OriGene's HSP70 expression vector (#RC200270) was used to transiently overexpress intracellular HSP70 in bronchial epithelial cells, enabling the study of its effects on glucocorticoid receptor expression and inflammatory signaling.
Snippet: C29), or (iv) eHSP70 plus TLR4 inhibitor (LPS-RS). 4.3. Plasmid Transfection NuLi-1 cells were transiently transfected with an expression vector for HSP70 (4 μg and 6 μg), which was purchased from OriGene (#RC200270, OriGene Technologies, Inc., Rockville, MD, USA), using Lipofectamine 2000 Transfection Reagent (#11668027, Invitrogen , Waltham, MA, USA) for 6 h. After transfection, cells we
Computational and cellular studies reveal structural destabilization and degradation of MLH1 variants in Lynch syndrome
Abildgaard, Stein, Nielsen et al
Elife (2019) 8 DOI: 10.7554/eLife.49138
Abildgaard, Stein, Nielsen et al
Elife (2019) 8 DOI: 10.7554/eLife.49138
AI Summary: In this study, the OriGene product pCMV6-MYC-DDK-HSP70-1A (HSPA1A) plasmid was used to investigate the role of chaperone-assisted degradation in the destabilization and loss of MLH1 protein variants associated with Lynch syndrome.
Snippet: Recombinant DNA reagent pCMV6-MYC-DDK-HSP70-1A (HSPA1A) OriGene RC200270 - Recombinant DNA reagent pcDNA3 -HA-HSP90 Addgene 22487;RRID:Addgene_22487 - Recombinant DNA reagent pEYFP-C2-PMS2 Prof. …
DNAJB3 attenuates metabolic stress and promotes glucose uptake by eliciting Glut4 translocation
Arredouani, Diane, Khattab et al
Sci Rep (2019) 9 (1), 4772 DOI: 10.1038/s41598-019-41244-8
Arredouani, Diane, Khattab et al
Sci Rep (2019) 9 (1), 4772 DOI: 10.1038/s41598-019-41244-8
AI Summary: OriGene products pCMV-DNAJB3 and pCMV-HSPA1A plasmids were used to overexpress human DNAJB3 and HSP-72 in cells, enabling the study of their effects on metabolic stress and glucose uptake in this research.
Snippet: All the cells were used before the 25 th passage. Plasmids and silencing RNA. pCMV-DNAJB3 and pCMV-HSPA1A plasmids were purchased from Origene (Origene Technologies, Inc., Rockvile, MD). They encode the human DNAJB3 and HSP-72, respectively
Mass spectrometry analyses of normal and polyglutamine expanded ataxin-3 reveal novel interaction partners involved in mitochondrial function
Kristensen, Oppermann, Rauen et al
Neurochem Int (2018) 112, 5-17 DOI: 10.1016/j.neuint.2017.10.013
Kristensen, Oppermann, Rauen et al
Neurochem Int (2018) 112, 5-17 DOI: 10.1016/j.neuint.2017.10.013
Cited by 24
AI Summary: OriGene products contributed to this study by providing the Myc-DDK-tagged ataxin-3 expression plasmids (OriGene, Cat# RC208706 and RC208707), which were used to express normal and polyglutamine-expanded ataxin-3 for protein interaction and mass spectrometry analyses.
Cell-free analysis of polyQ-dependent protein aggregation and its inhibition by chaperone proteins
Machida, Shigeta, Kobayashi et al
J. Biotechnol. (2016) 239, 1-8 DOI: 10.1016/j.jbiotec.2016.09.031
Machida, Shigeta, Kobayashi et al
J. Biotechnol. (2016) 239, 1-8 DOI: 10.1016/j.jbiotec.2016.09.031
AI Summary: OriGene contributed to this study by providing the cDNA for Hsp70A1A (NM_005345), which was used to investigate the role of chaperone proteins in inhibiting polyglutamine-dependent protein aggregation.
Mass spectrometry analyses of normal and polyglutamine expanded ataxin-3 reveal novel interaction partners involved in mitochondrial function
Kristensen, Oppermann, Rauen et al
Neurochemistry International (2017)
Kristensen, Oppermann, Rauen et al
Neurochemistry International (2017)
AI Summary: OriGene products CHIP-Myc-FLAG (RC200310), HSPA1A-Myc-FLAG (RC200270), BAG-1-Myc-FLAG (RC201272), and Hip-Myc-FLAG (RC220533) in pcMV6 vectors were used in this study to investigate protein interactions related to mitochondrial function in ataxin-3.
Snippet: Denmark). CHIP-Myc-FLAG (RC200310), HSPA1A-Myc-FLAG (RC200270), BAG-1-Myc-FLAG (RC201272), and Hip-Myc-FLAG (RC220533) all in pcMV6 vector, were purchased from Origene (Origene Technologies, USA);... Denmark). CHIP-Myc-FLAG (RC200310), HSPA1A-Myc-FLAG (RC200270), BAG-1-Myc-FLAG (RC201272), and Hip-Myc-FLAG (RC220533) all in pcMV6 vector, were purchased from Origene (Origene Technologies, USA).
Showing 1-6 of 6 papers.
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