TXNL4A (NM_006701) Human Tagged ORF Clone

  • TrueORF®
SKU
RC200266
TXNL4A (Myc-DDK-tagged)-Human thioredoxin-like 4A (TXNL4A)
  $289.00
2 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TXNL4A
Synonyms BMKS; DIB1; DIM1; SNRNP15; TXNL4; U5-15kD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200266 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGTACATGCTCCCGCACCTGCACAACGGCTGGCAGGTGGACCAGGCCATCCTCTCGGAGGAGGACC
GCGTGGTCGTCATCCGCTTCGGCCACGACTGGGATCCTACGTGCATGAAGATGGACGAGGTCCTGTACAG
CATCGCCGAGAAGGTTAAAAATTTTGCAGTTATTTATCTTGTGGATATTACAGAAGTGCCTGACTTCAAC
AAAATGTATGAGTTATACGATCCATGTACTGTCATGTTTTTCTTCAGGAACAAGCACATCATGATTGACT
TGGGGACTGGCAACAACAACAAGATTAACTGGGCCATGGAGGACAAGCAGGAGATGGTGGACATCATCGA
GACGGTGTACCGCGGGGCCCGCAAAGGCCGCGGCCTGGTGGTGTCCCCCAAGGACTACTCCACCAAGTAC
CGCTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC200266 protein sequence
Red=Cloning site Green=Tags(s)

MSYMLPHLHNGWQVDQAILSEEDRVVVIRFGHDWDPTCMKMDEVLYSIAEKVKNFAVIYLVDITEVPDFN
KMYELYDPCTVMFFFRNKHIMIDLGTGNNNKINWAMEDKQEMVDIIETVYRGARKGRGLVVSPKDYSTKY
RY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006701
ORF Size 426 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006701.5
RefSeq Size 1415 bp
RefSeq ORF 429 bp
Locus ID 10907
UniProt ID P83876
Cytogenetics 18q23
Domains DIM1
Protein Families Druggable Genome
Protein Pathways Spliceosome
MW 16.8 kDa
Summary The protein encoded by this gene is a member of the U5 small ribonucleoprotein particle (snRNP), and is involved in pre-mRNA splicing. This protein contains a thioredoxin-like fold and it is expected to interact with multiple proteins. Protein-protein interactions have been observed with the polyglutamine tract-binding protein 1 (PQBP1). Mutations in both the coding region and promoter region of this gene have been associated with Burn-McKeown syndrome, which is a rare disorder characterized by craniofacial dysmorphisms, cardiac defects, hearing loss, and bilateral choanal atresia. A pseudogene of this gene is found on chromosome 2. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2015
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Documents
Resources
"NM_006701" in other vectors (4)
SKU Description Size Price
RC200266L3 Lenti ORF clone of Human thioredoxin-like 4A (TXNL4A), Myc-DDK-tagged 10 ug
$450.00
RC200266L4 Lenti ORF clone of Human thioredoxin-like 4A (TXNL4A), mGFP tagged 10 ug
$450.00
RG200266 TXNL4A (tGFP-tagged) - Human thioredoxin-like 4A (TXNL4A) 10 ug
$489.00
SC115962 TXNL4A (untagged)-Human thioredoxin-like 4A (TXNL4A) 10 ug
$150.00

Plasmid Map for RC200266

Download sequence for

Cloning scheme for RC200266

Circular map for RC200266

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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