Flotillin 1 (FLOT1) (NM_005803) Human Tagged ORF Clone

SKU
RC200231
FLOT1 (Myc-DDK-tagged)-Human flotillin 1 (FLOT1)
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    Protein expression verified by Western blot, fully sequenced and in stock

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$457.00
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Specifications
Product Data
Target Symbol Flotillin 1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200231 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTTTCACTTGTGGCCCAAATGAGGCCATGGTGGTCTCCGGGTTCTGCCGAAGCCCCCCAGTCATGG
TGGCTGGAGGGCGTGTCTTTGTCCTGCCCTGCATCCAACAGATCCAGAGGATCTCTCTCAACACACTGAC
CCTCAATGTCAAGAGTGAAAAGGTTTACACTCGCCATGGGGTCCCCATCTCAGTCACTGGCATTGCCCAG
GTAAAAATCCAGGGGCAGAACAAGGAGATGTTGGCGGCCGCCTGTCAGATGTTCCTGGGGAAGACGGAGG
CTGAGATTGCCCACATTGCCCTGGAGACGTTAGAGGGCCACCAGAGGGCCATCATGGCCCACATGACTGT
GGAGGAGATCTATAAGGACAGGCAGAAATTCTCAGAACAGGTTTTCAAAGTGGCCTCCTCAGACCTGGTC
AACATGGGCATCAGTGTGGTTAGCTACACTCTGAAGGACATTCACGATGACCAGGACTATTTGCACTCTT
TGGGGAAGGCTCGAACAGCTCAAGTCCAAAAAGATGCACGGATTGGAGAAGCAGAGGCCAAGAGAGATGC
TGGGATCCGGGAAGCTAAAGCCAAGCAGGAAAAGGTGTCTGCTCAGTACCTGAGTGAGATCGAGATGGCC
AAGGCACAGAGAGATTACGAACTGAAGAAGGCCGCCTATGACATCGAGGTCAACACCCGCCGAGCACAGG
CTGACCTGGCCTATCAGCTTCAGGTGGCCAAGACTAAGCAGCAGATTGAGGAGCAGCGGGTGCAGGTGCA
GGTGGTGGAGCGGGCCCAGCAGGTGGCAGTGCAGGAGCAGGAGATCGCCCGGCGGGAGAAGGAGCTGGAG
GCCCGGGTGCGGAAGCCAGCGGAAGCGGAGCGCTACAAGCTGGAGCGCCTAGCCGAGGCAGAGAAGTCCC
AACTAATTATGCAGGCGGAGGCAGAAGCCGCGTCTGTGCGGATGCGTGGGGAAGCTGAGGCCTTTGCCAT
AGGGGCCCGAGCCCGAGCCGAGGCTGAGCAGATGGCCAAGAAGGCAGAAGCCTTCCAGCTGTACCAAGAG
GCTGCTCAGCTGGACATGCTGCTAGAGAAGCTGCCCCAGGTGGCAGAGGAGATCAGTGGTCCCTTGACTT
CAGCCAATAAGATCACACTGGTGTCCAGCGGCAGTGGGACCATGGGGGCAGCCAAAGTGACTGGGGAAGT
ACTGGACATTCTAACTCGCCTGCCAGAGAGTGTGGAAAGACTCACAGGCGTGAGCATCTCCCAGGTGAAT
CACAAGCCTTTGAGAACAGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC200231 protein sequence
Red=Cloning site Green=Tags(s)

MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQ
VKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLV
NMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMA
KAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELE
ARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQE
AAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVN
HKPLRTA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_005803
ORF Size 1281 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005803.4
RefSeq Size 1839 bp
RefSeq ORF 1284 bp
Locus ID 10211
UniProt ID O75955
Cytogenetics 6p21.33
Domains Band_7, Flotillin
Protein Pathways Insulin signaling pathway
MW 47.4 kDa
Summary This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
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SKU Description Size Price
RC200231L1 Lenti ORF clone of Human flotillin 1 (FLOT1), Myc-DDK-tagged 10 ug
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RC200231L3 Lenti ORF clone of Human flotillin 1 (FLOT1), Myc-DDK-tagged 10 ug
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RC200231L4 Lenti ORF clone of Human flotillin 1 (FLOT1), mGFP tagged 10 ug
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RG200231 FLOT1 (tGFP-tagged) - Human flotillin 1 (FLOT1) 10 ug
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.