Flotillin 1 (FLOT1) (NM_005803) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Flotillin 1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005803 |
| ORF Size | 1281 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005803.4 |
| RefSeq Size | 1839 bp |
| RefSeq ORF | 1284 bp |
| Locus ID | 10211 |
| UniProt ID | O75955 |
| Cytogenetics | 6p21.33 |
| Domains | Band_7, Flotillin |
| Protein Pathways | Insulin signaling pathway |
| MW | 47.4 kDa |
| Summary | This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jan 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005803" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200231L1 | Lenti ORF clone of Human flotillin 1 (FLOT1), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC200231L2 | Lenti ORF clone of Human flotillin 1 (FLOT1), mGFP tagged | 10 ug |
$757.00
|
|
| RC200231L3 | Lenti ORF clone of Human flotillin 1 (FLOT1), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC200231L4 | Lenti ORF clone of Human flotillin 1 (FLOT1), mGFP tagged | 10 ug |
$757.00
|
|
| RG200231 | FLOT1 (tGFP-tagged) - Human flotillin 1 (FLOT1) | 10 ug |
$489.00
$657.00
|
|
| SC319463 | FLOT1 (untagged)-Human flotillin 1 (FLOT1) | 10 ug |
$457.00
|
|
Citations
Flotillin-2 regulates epidermal growth factor receptor activation, degradation by Cbl-mediated ubiquitination, and cancer growth
Wisniewski, Liyasova, Korrapati et al
J Biol Chem (2022) 299 (1), 102766 DOI: 10.1016/j.jbc.2022.102766
Wisniewski, Liyasova, Korrapati et al
J Biol Chem (2022) 299 (1), 102766 DOI: 10.1016/j.jbc.2022.102766
Cited by 8
AI Summary: OriGene products contributed to this study by providing the overexpression plasmids pCMV6 (PS10001), FLOT1 (RC200231), and FLOT2 (RC220884), which were used to investigate the roles of Flotillin-1 and Flotillin-2 in regulating EGFR activation and signaling.
Snippet: r 3 h and then stimulated with 100 ng/ml EGF for up to 15 min, harvested, and lysed. Each experiment was repeated at least 3 times. Plasmids for overexpression in HEK293T cells were purchased from OriGene: pCMV6 (PS10001), FLOT1 (RC200231), FLOT2 (RC220884). The use of HA-tagged Cbl plasmid in pCEFL expression vector as well as WT EGFR in pcDNA3 vector has been reported (47). Site-directed muta
MAL2 mediates the formation of stable HER2 signaling complexes within lipid raft-rich membrane protrusions in breast cancer cells
Jeong, Shin, Li et al
Cell Rep (2021) 37 (13), 110160 DOI: 10.1016/j.celrep.2021.110160
Jeong, Shin, Li et al
Cell Rep (2021) 37 (13), 110160 DOI: 10.1016/j.celrep.2021.110160
Cited by 36
AI Summary: In this study, OriGene’s MAL2 shRNA (cat# TL313896) and MAL2 cDNA clone (cat# RC206307) were used to modulate MAL2 expression, enabling the authors to investigate MAL2’s role in HER2 signaling complex formation and membrane retention in breast cancer cells.
Snippet: The lipid raft-resident protein, MAL2, has been implicated as contributing to the pathogenesis of several malignancies, including breast cancer, but the underlying mechanism for its effects on tumorigenesis is unknown. Here, we show that MAL2-mediated lipid raft formation leads to HER2 plasma membrane retention and enhanced HER2 signaling in breast cancer cells. We demonstrate physical interactions between HER2 and MAL2 in lipid rafts using proximity ligation assays. …HER2/MAL2 protein interactions in lipid rafts are enhanced in trastuzumab-resistant breast cancer cells. Our findings suggest that MAL2 is crucial for lipid raft formation, HER2 signaling, and HER2 membrane stability in breast cancer cells, suggesting MAL2 as a potential therapeutic target.Copyright 2021 The Authors. …
Showing 1-2 of 2 papers.
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