SIRT5 (NM_012241) Human Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SIRT5 |
| Synonyms | SIR2L5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012241 |
| ORF Size | 930 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012241.5 |
| RefSeq Size | 4538 bp |
| RefSeq ORF | 933 bp |
| Locus ID | 23408 |
| UniProt ID | Q9NXA8, A0A024R012 |
| Cytogenetics | 6p23 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 33.9 kDa |
| Summary | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, Jul 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012241" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200189L1 | Lenti ORF clone of Human sirtuin 5 (SIRT5), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC200189L2 | Lenti ORF clone of Human sirtuin 5 (SIRT5), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RC200189L3 | Lenti ORF clone of Human sirtuin 5 (SIRT5), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC200189L4 | Lenti ORF clone of Human sirtuin 5 (SIRT5), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RG200189 | SIRT5 (tGFP-tagged) - Human sirtuin 5 (SIRT5), transcript variant 1 | 10 ug |
$650.00
|
|
| SC321579 | SIRT5 (untagged)-Human sirtuin 5 (SIRT5), transcript variant 1 | 10 ug |
$450.00
|
|
Citations
Sirtuin 5 regulates the proliferation, invasion and migration of prostate cancer cells through acetyl-CoA acetyltransferase 1
Guan, Jiang, Gai et al
J Cell Mol Med (2020)
Guan, Jiang, Gai et al
J Cell Mol Med (2020)
Cited by 42
AI Summary: OriGene products Flag‐SIRT5 (RC200189) plasmid and its control Flag‐pCMV6 were used in this study to manipulate SIRT5 expression and investigate its role in prostate cancer cell proliferation, migration, and invasion.
Snippet: Flag‐SIRT5 (RC200189) is an SIRT5 up‐regulated plasmid, and its control is Flag‐pCMV6;both were procured from Origene (Rockville, MD, USA). ACAT1‐3Flag, the overexpression plasmid of ACAT1, and its control, GV141, were obtained from GENE (Shanghai;- Flag‐SIRT5 (RC200189) is an SIRT5 up‐regulated plasmid, and its control is Flag‐pCMV6
SIRT5 Promotes Cisplatin Resistance in Ovarian Cancer by Suppressing DNA Damage in a ROS-Dependent Manner via Regulation of the Nrf2/HO-1 Pathway
Sun, Wang, Gai et al
Front Oncol (2019) 9, 754 DOI: 10.3389/fonc.2019.00754
Sun, Wang, Gai et al
Front Oncol (2019) 9, 754 DOI: 10.3389/fonc.2019.00754
AI Summary: OriGene products, specifically the SIRT5 expression plasmid and the corresponding empty plasmid, were used to overexpress SIRT5 and as a negative control, respectively, enabling the study of SIRT5’s role in cisplatin resistance in ovarian cancer cells.
Snippet: manufacturer's instructions. A SIRT5 expression plasmid (OriGene, Rockville, MD, USA) and the corresponding empty plasmid (OriGene) were used for SIRT5 overexpression and as a negative control, respectively. Cells were Cached
Drosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase β and regulates complex V activity
Rahman, Nirala, Singh et al
J. Cell Biol. (2014) 206 (2), 289-305 DOI: 10.1083/jcb.201404118
Rahman, Nirala, Singh et al
J. Cell Biol. (2014) 206 (2), 289-305 DOI: 10.1083/jcb.201404118
Cited by 116
Showing 1-3 of 3 papers.
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