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TENT5A (NM_017633) Human Tagged ORF Clone

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SKU
RC200181
FAM46A (Myc-DDK-tagged)-Human family with sequence similarity 46, member A (FAM46A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "TENT5A or NM_017633"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TENT5A
Synonyms C6orf37; FAM46A; OI18; XTP11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200181 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGGGTGAAGGGTACTTCGCCATGTCTGAGGACGAGCTGGCCTGCAGCCCCTACATCCCCCTAG
GCGGCGACTTCGGCGGCGGCGACTTCGGCGGCGGCGACTTCGGCGGCGGCGACTTCGGCGGTGGCGGCAG
CTTCGGTGGGCATTGCTTGGACTATTGCGAAAGCCCTACGGCGCACTGCAATGTGCTGAACTGGGAGCAA
GTGCAGCGGCTGGACGGCATCCTGAGCGAGACCATTCCGATTCACGGGCGCGGCAACTTCCCCACGCTCG
AGCTGCAGCCGAGCCTGATCGTGAAGGTGGTGCGGCGGCGCCTGGCCGAGAAGCGCATTGGCGTCCGCGA
CGTGCGCCTCAACGGCTCGGCAGCCAGCCATGTCCTGCACCAGGACAGCGGCCTGGGCTACAAGGACCTG
GACCTCATCTTCTGCGCCGACCTGCGCGGGGAAGGGGAGTTTCAGACTGTGAAGGACGTCGTGCTGGACT
GCCTGTTGGACTTCTTACCCGAGGGGGTGAACAAAGAGAAGATCACACCACTCACGCTCAAGGAAGCTTA
TGTGCAGAAAATGGTTAAAGTGTGCAATGACTCTGACCGATGGAGTCTTATATCCCTGTCAAACAACAGT
GGCAAAAATGTGGAACTGAAATTTGTGGATTCCCTCCGGAGGCAGTTTGAATTCAGTGTAGATTCTTTTC
AAATCAAATTAGACTCTCTTCTGCTCTTTTATGAATGTTCAGAGAACCCAATGACTGAGACATTTCACCC
CACAATAATCGGGGAGAGCGTCTATGGCGATTTCCAGGAAGCCTTTGATCACCTTTGTAACAAGATCATT
GCCACCAGGAACCCAGAGGAAATCCGAGGGGGAGGCCTGCTTAAGTACTGCAACCTCTTGGTGAGGGGCT
TTAGGCCCGCCTCTGATGAAATCAAGACCCTTCAAAGGTATATGTGTTCCAGGTTTTTCATCGACTTCTC
AGACATTGGAGAGCAGCAGAGAAAACTGGAGTCCTATTTGCAGAACCACTTTGTGGGATTGGAAGACCGC
AAGTATGAGTATCTCATGACCCTTCATGGAGTGGTAAATGAGAGCACAGTGTGCCTGATGGGACATGAAA
GAAGACAGACTTTAAACCTTATCACCATGCTGGCTATCCGGGTGTTAGCTGACCAAAATGTCATTCCTAA
TGTGGCTAATGTCACTTGCTATTACCAGCCAGCCCCCTATGTAGCAGATGCCAACTTTAGCAATTACTAC
ATTGCACAGGTTCAGCCAGTATTCACGTGCCAGCAACAGACCTACTCCACTTGGCTACCCTGCAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200181 protein sequence
Red=Cloning site Green=Tags(s)

MAEGEGYFAMSEDELACSPYIPLGGDFGGGDFGGGDFGGGDFGGGGSFGGHCLDYCESPTAHCNVLNWEQ
VQRLDGILSETIPIHGRGNFPTLELQPSLIVKVVRRRLAEKRIGVRDVRLNGSAASHVLHQDSGLGYKDL
DLIFCADLRGEGEFQTVKDVVLDCLLDFLPEGVNKEKITPLTLKEAYVQKMVKVCNDSDRWSLISLSNNS
GKNVELKFVDSLRRQFEFSVDSFQIKLDSLLLFYECSENPMTETFHPTIIGESVYGDFQEAFDHLCNKII
ATRNPEEIRGGGLLKYCNLLVRGFRPASDEIKTLQRYMCSRFFIDFSDIGEQQRKLESYLQNHFVGLEDR
KYEYLMTLHGVVNESTVCLMGHERRQTLNLITMLAIRVLADQNVIPNVANVTCYYQPAPYVADANFSNYY
IAQVQPVFTCQQQTYSTWLPCN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017633
ORF Size 1326 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017633.3
RefSeq Size 5617 bp
RefSeq ORF 1329 bp
Locus ID 55603
UniProt ID Q96IP4
Cytogenetics 6q14.1
MW 49.7 kDa
Summary Probable nucleotidyltransferase that may act as a non-canonical poly(A) RNA polymerase.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_017633" in other vectors (6)
SKU Description Size Price
RC200181L1 Lenti ORF clone of Human family with sequence similarity 46, member A (FAM46A), Myc-DDK-tagged 10 ug
$757.00
RC200181L2 Lenti ORF clone of Human family with sequence similarity 46, member A (FAM46A), mGFP tagged 10 ug
$757.00
RC200181L3 Lenti ORF clone of Human family with sequence similarity 46, member A (FAM46A), Myc-DDK-tagged 10 ug
$757.00
RC200181L4 Lenti ORF clone of Human family with sequence similarity 46, member A (FAM46A), mGFP tagged 10 ug
$757.00
RG200181 FAM46A (tGFP-tagged) - Human family with sequence similarity 46, member A (FAM46A) 10 ug
$489.00 $657.00
SC321318 FAM46A (untagged)-Human family with sequence similarity 46, member A (FAM46A) 10 ug
$457.00

Plasmid Map for RC200181

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200181
Circular map for RC200181

file-alt Citations

Protein quantitative trait locus study in obesity during weight-loss identifies a leptin regulator
Carayol, Chabert, Di Cara et al
Nat Commun (2017) 8 (1), 2084 DOI: 10.1038/s41467-017-02182-z
AI Summary: OriGene’s FAM46A (NM_017633) full human cDNA ORF Clone was used to overexpress FAM46A in adipocytes, enabling the study to validate FAM46A as a trans regulator of leptin in obesity-related protein regulation.
Snippet: Briefly, on day 7 of differentiation, SGBS cells were detached with trypsin/EDTA and counted. pCMV6 empty vector (Control) or FAM46A (NM_017633) full human cDNA ORF Clone (OriGene, Rockville, MD, USA) were delivered into adipocytes at a concentration of 0.5 µg per 400,000 cells with the Neon Transfection System (Invitrogen, Madison, WI, USA)
FigureFigureFigure
Showing 1-1 of 1 papers.
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