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KCTD5 (NM_018992) Human Tagged ORF Clone

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SKU
RC200180
KCTD5 (Myc-DDK-tagged)-Human potassium channel tetramerisation domain containing 5 (KCTD5)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "KCTD5 or NM_018992"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KCTD5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200180 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGAATCACTGCGAGCTCCTGTCGCCGGCCCGGGGCGGCATCGGGGCGGGGCTGGGGGGCGGCC
TGTGCCGCCGCTGCAGCGCTGGGCTCGGCGCCCTGGCCCAGCGCCCTGGCAGCGTGTCCAAGTGGGTCCG
ACTCAACGTCGGCGGCACCTACTTCCTCACCACTCGGCAGACCCTGTGCCGGGACCCGAAATCCTTCCTG
TACCGCTTATGCCAGGCCGATCCCGACCTGGACTCAGACAAGGATGAAACAGGCGCCTATTTAATCGACA
GAGACCCCACCTACTTTGGGCCTGTGCTGAACTACCTGAGACACGGCAAGCTGGTGATTAACAAAGACCT
CGCGGAGGAAGGAGTGTTGGAGGAAGCAGAATTTTACAATATCACCTCATTAATAAAACTTGTAAAGGAC
AAAATTAGAGAACGAGACAGCAAAACATCGCAGGTGCCTGTGAAGCATGTGTACCGTGTGCTGCAGTGCC
AGGAGGAGGAGCTCACGCAGATGGTGTCCACCATGTCCGACGGCTGGAAGTTCGAGCAGTTGGTCAGCAT
CGGCTCCTCTTACAACTATGGGAACGAAGACCAAGCCGAGTTCCTCTGTGTGGTGTCCAAGGAGCTGCAC
AACACCCCGTACGGTACGGCCAGCGAGCCCAGCGAGAAGGCCAAGATTTTGCAAGAACGAGGCTCAAGGA
TG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200180 protein sequence
Red=Cloning site Green=Tags(s)

MAENHCELLSPARGGIGAGLGGGLCRRCSAGLGALAQRPGSVSKWVRLNVGGTYFLTTRQTLCRDPKSFL
YRLCQADPDLDSDKDETGAYLIDRDPTYFGPVLNYLRHGKLVINKDLAEEGVLEEAEFYNITSLIKLVKD
KIRERDSKTSQVPVKHVYRVLQCQEEELTQMVSTMSDGWKFEQLVSIGSSYNYGNEDQAEFLCVVSKELH
NTPYGTASEPSEKAKILQERGSRM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018992
ORF Size 702 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018992.4
RefSeq Size 2479 bp
RefSeq ORF 705 bp
Locus ID 54442
UniProt ID Q9NXV2
Cytogenetics 16p13.3
Domains BTB, K_tetra
Protein Families Ion Channels: Other
MW 26.1 kDa
Summary Its interaction with CUL3 suggests that it may act as a substrate adapter in some E3 ligase complex (PubMed:18573101). Does not affect the function of Kv channel Kv2.1/KCNB1, Kv1.2/KCNA2, Kv4.2/KCND2 and Kv3.4/KCNC4 (PubMed:19361449).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_018992" in other vectors (7)
SKU Description Size Price
RC200180L1 Lenti ORF clone of Human potassium channel tetramerisation domain containing 5 (KCTD5), Myc-DDK-tagged 10 ug
$600.00
RC200180L2 Lenti ORF clone of Human potassium channel tetramerisation domain containing 5 (KCTD5), mGFP tagged 10 ug
$600.00
RC200180L3 Lenti ORF clone of Human potassium channel tetramerisation domain containing 5 (KCTD5), Myc-DDK-tagged 10 ug
$600.00
RC200180L4 Lenti ORF clone of Human potassium channel tetramerisation domain containing 5 (KCTD5), mGFP tagged 10 ug
$600.00
RG200180 KCTD5 (tGFP-tagged) - Human potassium channel tetramerisation domain containing 5 (KCTD5) 10 ug
$489.00 $500.00
SC310612 KCTD5 (untagged)-Human potassium channel tetramerisation domain containing 5 (KCTD5) 10 ug
$330.00
SC320723 KCTD5 (untagged)-Human potassium channel tetramerisation domain containing 5 (KCTD5) 10 ug
$300.00

Plasmid Map for RC200180

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200180
Circular map for RC200180

file-alt Citations

Multiple potassium channel tetramerization domain (KCTD) family members interact with Gβγ, with effects on cAMP signaling
Sloan, Cryan, Muntean
J Biol Chem (2023) 299 (3), 102924 DOI: 10.1016/j.jbc.2023.102924
Cited by 25
AI Summary: In this study, OriGene products—including Myc-DDK-tagged human KCTD2, KCTD5, and KCTD17 expression plasmids—were used to investigate the interactions between KCTD family proteins and Gβγ subunits, enabling the authors to characterize how these proteins modulate cAMP signaling pathways.
FigureFigureFigure
Multiple Potassium Channel Tetramerization Domain (KCTD) family members interact with Gβγ, with effects on cAMP signaling
Sloan, Cryan, Muntean
Journal of Biological Chemistry (2023) 299 (3), 102924 DOI: 10.1016/j.jbc.2023.102924
Cited by 25
AI Summary: OriGene products KCTD2-myc (RC218957), KCTD5-myc (RC200180), KCTD8-myc (RC223904), and KCTD12-myc (RC204577) were used as expression constructs to study the interactions between KCTD family proteins and Gβγ, helping to elucidate their role in modulating cAMP signaling.
Snippet: ), Venus 156-239-Gβ1 (27), Venus 1-155-Gγ2 (27), pmCherry-N1 (Takara Bio;catalog no.: 632523), pmVenus-N1 (68), masGRK3ct (69), masGRK3ct-Nluc (29), PTX-S1 (70), TEpacVV (31), AC5 (37), KCTD2-myc (Origene;catalog no.: RC218957), KCTD5-myc (Origene;catalog no.: RC200180), KCTD8-myc (Origene;catalog no.: RC223904), KCTD12-myc (Origene;catalog no.: RC204577), KCTD16-myc (Origene;catalog no.:
Showing 1-2 of 2 papers.
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