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FBXL12 (NM_017703) Human Tagged ORF Clone

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SKU
RC200178
FBXL12 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 12 (FBXL12)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FBXL12
Synonyms Fbl12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200178 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACTTTGGTCGAACTGCCGGACTCGGTCCTGCTCGAGATCTTCTCTTACCTCCCGGTACGGGACC
GGATCCGCATCTCCAGGGTCTGTCACCGCTGGAAGAGGCTGGTGGACGACCGGTGGCTGTGGCGACATGT
CGACCTGACGCTCTACACGATGCGACCTAAAGTCATGTGGCACCTCCTTCGAAGGTACATGGCATCCCGG
CTCCATTCCCTGCGGATGGGTGGCTACCTGTTCTCTGGCTCCCAGGCCCCCCAGTTGTCCCCTGCTCTGT
TGAGAGCCCTGGGCCAGAAGTGCCCCAACCTGAAGCGCCTCTGCCTGCATGTGGCCGACCTGAGCATGGT
GCCCATCACCAGCCTGCCCAGCACCTTGAGGACCCTGGAGCTGCACAGCTGCGAGATCTCCATGGCCTGG
CTCCACAAGCAGCAGGACCCCACCGTGCTGCCCCTGCTTGAATGCATCGTGCTGGACCGCGTCCCCGCCT
TCCGTGACGAGCACCTGCAGGGCCTGACGCGCTTCCGGGCCTTGCGCTCGCTGGTGCTGGGTGGTACCTA
CCGTGTGACCGAGACAGGGCTGGATGCTGGCCTGCAGGAGCTCAGCTATCTGCAGAGGCTTGAGGTGCTG
GGCTGCACCCTGTCTGCCGACAGCACCCTGCTGGCCATCAGCCGCCACCTCCGAGATGTGCGCAAGATCC
GGCTGACCGTGAGGGGCCTCTCTGCCCCTGGCCTGGCTGTGCTGGAGGGAATGCCGGCCCTGGAGAGTCT
GTGCCTGCAGGGTCCCCTCGTCACCCCAGAAATGCCCTCCCCCACTGAAATCCTCTCCTCCTGCCTCACT
ATGCCCAAGCTCAGAGTCCTTGAGCTGCAGGGGCTGGGGTGGGAGGGTCAGGAGGCGGAGAAGATCCTGT
GTAAGGGGCTGCCCCACTGTATGGTCATCGTCAGGGCTTGCCCCAAAGAGTCTATGGACTGGTGGATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200178 protein sequence
Red=Cloning site Green=Tags(s)

MATLVELPDSVLLEIFSYLPVRDRIRISRVCHRWKRLVDDRWLWRHVDLTLYTMRPKVMWHLLRRYMASR
LHSLRMGGYLFSGSQAPQLSPALLRALGQKCPNLKRLCLHVADLSMVPITSLPSTLRTLELHSCEISMAW
LHKQQDPTVLPLLECIVLDRVPAFRDEHLQGLTRFRALRSLVLGGTYRVTETGLDAGLQELSYLQRLEVL
GCTLSADSTLLAISRHLRDVRKIRLTVRGLSAPGLAVLEGMPALESLCLQGPLVTPEMPSPTEILSSCLT
MPKLRVLELQGLGWEGQEAEKILCKGLPHCMVIVRACPKESMDWWM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017703
ORF Size 978 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017703.3
RefSeq Size 1876 bp
RefSeq ORF 981 bp
Locus ID 54850
UniProt ID Q9NXK8
Cytogenetics 19p13.2
Domains F-box
Protein Families Druggable Genome
MW 37 kDa
Summary Members of the F-box protein family, such as FBXL12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 PubMed 15520277).supplied by OMIM, Mar 2008
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"NM_017703" in other vectors (6)
SKU Description Size Price
RC200178L1 Lenti ORF clone of Human F-box and leucine-rich repeat protein 12 (FBXL12), Myc-DDK-tagged 10 ug
$600.00
RC200178L2 Lenti ORF clone of Human F-box and leucine-rich repeat protein 12 (FBXL12), mGFP tagged 10 ug
$600.00
RC200178L3 Lenti ORF clone of Human F-box and leucine-rich repeat protein 12 (FBXL12), Myc-DDK-tagged 10 ug
$600.00
RC200178L4 Lenti ORF clone of Human F-box and leucine-rich repeat protein 12 (FBXL12), mGFP tagged 10 ug
$600.00
RG200178 FBXL12 (tGFP-tagged) - Human F-box and leucine-rich repeat protein 12 (FBXL12) 10 ug
$489.00 $500.00
SC113988 FBXL12 (untagged)-Human F-box and leucine-rich repeat protein 12 (FBXL12) 10 ug
$300.00

Plasmid Map for RC200178

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200178
Circular map for RC200178

file-alt Citations

Human Endogenous Retrovirus K Envelope in Spinal Fluid of Amyotrophic Lateral Sclerosis Is Toxic
Steiner, Bachani, Malik et al
Ann Neurol (2022) 92 (4), 545-561 DOI: 10.1002/ana.26452
Cited by 54
AI Summary: OriGene’s CD98 plasmid (RC200178) was used to transfect 293T cells, enabling the study of HERV-K Env protein binding and neurotoxicity mechanisms in this ALS research.
Snippet: …Immunoprecipitation and Western Blot Analysis A total of 293 T cells were transfected with CD98 (OriGene, Rockville, MD;RC200178) using Lipofectamine 3000. … One million cells/immunoprecipitation were lys;A total of 293 T cells were transfected with CD98 (OriGene, Rockville, MD;RC200178) using Lipofectamine 3000. …
Showing 1-1 of 1 papers.
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