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ELOVL1 (NM_022821) Human Tagged ORF Clone

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SKU
RC200027
ELOVL1 (Myc-DDK-tagged)-Human ELOVL fatty acid elongase 1 (ELOVL1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ELOVL1
Synonyms CGI-88; IKSHD; Ssc1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200027 representing NM_022821
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCTGTTGTGAACTTGTACCAAGAGGTGATGAAGCACGCAGATCCCCGGATCCAGGGCTACCCTC
TGATGGGGTCCCCCTTGCTAATGACCTCCATTCTCCTGACCTACGTGTACTTCGTTCTCTCACTTGGGCC
TCGCATCATGGCTAATCGGAAGCCCTTCCAGCTCCGTGGCTTCATGATTGTCTACAACTTCTCACTGGTG
GCACTCTCCCTCTACATTGTCTATGAGTTCCTGATGTCGGGCTGGCTGAGCACCTATACCTGGCGCTGTG
ACCCTGTGGACTATTCCAACAGCCCTGAGGCACTTAGGATGGTTCGGGTGGCCTGGCTCTTCCTCTTCTC
CAAGTTCATTGAGCTGATGGACACAGTGATCTTTATTCTCCGAAAGAAAGACGGGCAGGTGACCTTCCTA
CATGTCTTCCATCACTCTGTGCTTCCCTGGAGCTGGTGGTGGGGGGTAAAGATTGCCCCGGGAGGAATGG
GCTCTTTCCATGCCATGATAAACTCTTCCGTGCATGTCATAATGTACCTGTACTACGGATTATCTGCCTT
TGGCCCTGTGGCACAACCCTACCTTTGGTGGAAAAAGCACATGACAGCCATTCAGCTGATCCAGTTTGTC
CTGGTCTCACTGCACATCTCCCAGTACTACTTTATGTCCAGCTGTAACTACCAGTACCCAGTCATTATTC
ACCTCATCTGGATGTATGGCACCATCTTCTTCATGCTGTTCTCCAACTTCTGGTATCACTCTTATACCAA
GGGCAAGCGGCTGCCCCGTGCACTTCAGCAAAATGGAGCTCCAGGTATTGCCAAGGTCAAGGCCAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200027 representing NM_022821
Red=Cloning site Green=Tags(s)

MEAVVNLYQEVMKHADPRIQGYPLMGSPLLMTSILLTYVYFVLSLGPRIMANRKPFQLRGFMIVYNFSLV
ALSLYIVYEFLMSGWLSTYTWRCDPVDYSNSPEALRMVRVAWLFLFSKFIELMDTVIFILRKKDGQVTFL
HVFHHSVLPWSWWWGVKIAPGGMGSFHAMINSSVHVIMYLYYGLSAFGPVAQPYLWWKKHMTAIQLIQFV
LVSLHISQYYFMSSCNYQYPVIIHLIWMYGTIFFMLFSNFWYHSYTKGKRLPRALQQNGAPGIAKVKAN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022821
ORF Size 837 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022821.4
RefSeq Size 1501 bp
RefSeq ORF 840 bp
Locus ID 64834
UniProt ID Q9BW60
Cytogenetics 1p34.2
Domains ELO
Protein Families Transmembrane
MW 32.5 kDa
Summary Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that exhibits activity toward saturated and monounsaturated acyl-CoA substrates, with the highest activity towards C22:0 acyl-CoA. May participate in the production of both saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. Important for saturated C24:0 and monounsaturated C24:1 sphingolipid synthesis. Indirectly inhibits RPE65 via production of VLCFAs.UniProtKB/Swiss-Prot Function
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"NM_022821" in other vectors (6)
SKU Description Size Price
RC200027L1 Lenti ORF clone of Human ELOVL fatty acid elongase 1 (ELOVL1), Myc-DDK-tagged 10 ug
$600.00
RC200027L2 Lenti ORF clone of Human ELOVL fatty acid elongase 1 (ELOVL1), mGFP tagged 10 ug
$600.00
RC200027L3 Lenti ORF clone of Human ELOVL fatty acid elongase 1 (ELOVL1), Myc-DDK-tagged 10 ug
$600.00
RC200027L4 Lenti ORF clone of Human ELOVL fatty acid elongase 1 (ELOVL1), mGFP tagged 10 ug
$600.00
RG200027 ELOVL1 (tGFP-tagged) - Human ELOVL fatty acid elongase 1 (ELOVL1) 10 ug
$489.00 $500.00
SC319359 ELOVL1 (untagged)-Human ELOVL fatty acid elongase 1 (ELOVL1) 10 ug
$300.00

Plasmid Map for RC200027

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200027
Circular map for RC200027

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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